Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F204 I0 R1	1155	255.8	8356456	95.7%	7997128	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,271,906	C→T	38.0%	E366E (GAG→GAA)	yjcF ←	pentapeptide repeats protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,271,906	0	C	T	38.0%	246.7 / 257.7	229	E366E (GAG→GAA)	yjcF	pentapeptide repeats protein
Reads supporting (aligned to +/- strand): ref base C (54/87); new base T (39/47); total (93/136)
Fisher's exact test for biased strand distribution p-value = 3.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 231 total aligned reads displayed.

GCATGAAGATTATCCTCAGTTTGTGACATGCCAGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACATTAGCCATATTAGAGTCGGTACATATAACCATTTCGAGGAAC  >  CP009273/4271757‑4272053

gCATGAAGATTATCCTCAGTTTGTGACATGCCAGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGcc                                                                                                                                                     <  1:174580/150‑1 (MQ=255)

  aTGAAGATTATCCTCAGTTTGTGACGTGCCAGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTc                                                                                                                                                   >  2:3144825/1‑150 (MQ=255)

    gAAGATTATCCTCAGTTTGTGACATGCCAGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCAt                                                                                                                                                 >  1:1041424/1‑150 (MQ=255)

      aGATTATCCTCAGTTTGTGACATGCCAGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCGTCATCg                                                                                                                                               <  1:1425463/150‑1 (MQ=255)

       gATTATCCTCAGTTTGTGACATGCCAGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGa                                                                                                                                              >  2:3990818/1‑150 (MQ=255)

        aTTATCCTCAGTTTGTGACATGCCAGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCTTCATCGaa                                                                                                                                             >  2:3960638/1‑150 (MQ=255)

           aTCCTCAGTTTGTGACATGCCAGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAAtt                                                                                                                                          <  1:3169037/150‑1 (MQ=255)

           aTCCTCAGTTTGTGACATGCCAGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAAtt                                                                                                                                          <  1:1251191/150‑1 (MQ=255)

           aTCCTCAGTTTGTGACATGCCAGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAAtt                                                                                                                                          <  1:2751479/150‑1 (MQ=255)

              cacAGTTTGTGACATGCCAGTATGAGTGGCACAATCTTCTTAAAAGAATTTCTGTAGTGTTACCAGGATATCAATATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACACATTCCCTGGACGCTAGCCTCATCGAATTTAac                                                                                                                                       <  2:297268/148‑1 (MQ=255)

                   tttGTGACATGCCAGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGAt                                                                                                                                  <  1:47025/150‑1 (MQ=255)

                     tgtgACATGCCAGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATag                                                                                                                                <  1:742209/150‑1 (MQ=255)

                     tgtgACATGCCAGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATag                                                                                                                                >  2:2052506/1‑150 (MQ=255)

                      gtgACATGCCAGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATaga                                                                                                                               <  1:3407761/150‑1 (MQ=255)

                      gtgACATGCCAGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATaga                                                                                                                               <  1:3546605/150‑1 (MQ=255)

                           aTGCCAGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACgg                                                                                                                          >  2:1664499/1‑150 (MQ=255)

                               cAGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACGGatat                                                                                                                      >  1:2921328/1‑150 (MQ=255)

                               cAGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACGGatat                                                                                                                      >  2:4130941/1‑150 (MQ=255)

                               cAGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGatat                                                                                                                      <  2:3914080/150‑1 (MQ=255)

                                   aTGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTaaa                                                                                                                  >  1:3567984/1‑150 (MQ=255)

                                         ggCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCa                                                                                                            >  1:2218564/1‑150 (MQ=255)

                                          gCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGCTATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAg                                                                                                           <  1:279438/150‑1 (MQ=255)

                                          gCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAg                                                                                                           <  2:3690300/150‑1 (MQ=255)

                                          gCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATAAATGGAGATCTTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAg                                                                                                           >  2:433714/1‑150 (MQ=255)

                                           cacaATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAgg                                                                                                          >  1:2570114/1‑150 (MQ=255)

                                            acaATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGc                                                                                                         >  2:1842760/1‑150 (MQ=255)

                                            acaATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGc                                                                                                         >  1:1081503/1‑150 (MQ=255)

                                              aaTCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCtt                                                                                                       >  1:2905187/1‑150 (MQ=255)

                                              aaTCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCtt                                                                                                       >  2:2614756/1‑150 (MQ=255)

                                              aaTCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCtt                                                                                                       <  1:628789/150‑1 (MQ=255)

                                                    cttcAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTa                                                                                                 <  2:3283118/150‑1 (MQ=255)

                                                         aaGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAaaca                                                                                            <  2:3071397/150‑1 (MQ=255)

                                                          aGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTGTTGTTAAaacag                                                                                           >  2:3949416/1‑150 (MQ=255)

                                                          aGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAaaaag                                                                                           <  1:3304970/150‑1 (MQ=255)

                                                            aaTTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAaacagaa                                                                                         >  2:2019979/1‑150 (MQ=255)

                                                             aTTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAaacagaac                                                                                        >  2:3425946/1‑150 (MQ=255)

                                                             aTTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAaacagaac                                                                                        >  1:2777445/1‑150 (MQ=255)

                                                              tttCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAaacagaaca                                                                                       >  2:379315/1‑150 (MQ=255)

                                                                       tgtTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATg                                                                              >  2:1037688/1‑150 (MQ=255)

                                                                       tgtTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATg                                                                              <  1:1966257/150‑1 (MQ=255)

                                                                           aCCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCaa                                                                          >  2:3859694/1‑150 (MQ=255)

                                                                            ccAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAAt                                                                         >  1:1603227/1‑150 (MQ=255)

                                                                            ccAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAAt                                                                         >  2:2169997/1‑150 (MQ=255)

                                                                              atgATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTg                                                                       <  2:2414284/148‑1 (MQ=255)

                                                                              aGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTg                                                                       >  2:910182/1‑150 (MQ=255)

                                                                               ggATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGc                                                                      >  1:1954748/1‑150 (MQ=255)

                                                                               ggATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGc                                                                      <  1:2547670/150‑1 (MQ=255)

                                                                                 atatCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCtt                                                                    <  2:1202942/150‑1 (MQ=255)

                                                                                 atatCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCtt                                                                    >  1:2517116/1‑150 (MQ=255)

                                                                                  tatCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTa                                                                   <  1:3964349/150‑1 (MQ=255)

                                                                                   atCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTaa                                                                  <  1:3839040/150‑1 (MQ=255)

                                                                                   atCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTaa                                                                  <  2:1435234/150‑1 (MQ=255)

                                                                                    tCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTaaa                                                                 <  1:871613/150‑1 (MQ=255)

                                                                                     cACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAAt                                                                <  2:2380857/150‑1 (MQ=255)

                                                                                     cACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAAt                                                                <  1:463083/150‑1 (MQ=255)

                                                                                       cTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTg                                                              <  2:1752205/150‑1 (MQ=255)

                                                                                         aTTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGc                                                            <  1:1664499/150‑1 (MQ=255)

                                                                                           tAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCat                                                          >  2:1436826/1‑150 (MQ=255)

                                                                                            aaTGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCatt                                                         >  1:130447/1‑150 (MQ=255)

                                                                                             aTGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCatta                                                        <  1:3520398/150‑1 (MQ=255)

                                                                                              tGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCattat                                                       <  1:4162952/150‑1 (MQ=255)

                                                                                               ggAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCattatt                                                      <  2:4028715/150‑1 (MQ=255)

                                                                                               ggAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCattatt                                                      >  1:1049491/1‑150 (MQ=255)

                                                                                                gagaTATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCAttatta                                                     >  2:1269321/1‑150 (MQ=255)

                                                                                                 agaTATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTaa                                                    >  2:702485/1‑150 (MQ=255)

                                                                                                   atatTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTaaaa                                                  <  1:896895/150‑1 (MQ=255)

                                                                                                         tCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACa                                            >  1:2286272/1‑150 (MQ=255)

                                                                                                          ccTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACAt                                           <  1:4062248/150‑1 (MQ=255)

                                                                                                          ccTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACAt                                           >  1:148868/1‑150 (MQ=255)

                                                                                                            tCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACATTa                                         >  2:1651150/1‑150 (MQ=255)

                                                                                                             caccacGGAGAATGGTAATACCCATTCCCTGGACGCTATCTTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACATTAg                                        <  1:2970753/150‑1 (MQ=255)

                                                                                                             caccacGGAGAATGGTAATACCCATTCCCTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACATTAg                                        <  1:2970860/150‑1 (MQ=255)

                                                                                                              accacGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACATTAGc                                       <  2:415199/150‑1 (MQ=255)

                                                                                                               ccacGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAATATTAACATTAGcc                                      >  1:926591/1‑150 (MQ=255)

                                                                                                                cacGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACATTAGCCa                                     <  2:259764/150‑1 (MQ=255)

                                                                                                                 acGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACATTAGCCat                                    <  2:4070773/150‑1 (MQ=255)

                                                                                                                      gaATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACATTAGCCATATTag                               <  1:1002044/150‑1 (MQ=255)

                                                                                                                       aaTGGTAATACCCATTCCCTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACATTAGCCATATTaga                              <  2:280860/150‑1 (MQ=255)

                                                                                                                       aaTGGTAATACCCATTCCCTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACATTAGCCATATTaga                              >  2:1571760/1‑150 (MQ=255)

                                                                                                                       aaTGGTAATACCCATTCCCTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACATTAGCCATATTaga                              <  2:1967763/150‑1 (MQ=255)

                                                                                                                       aaTGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACATTAGCCATATTaga                              >  1:4018686/1‑150 (MQ=255)

                                                                                                                        aTGGTAATACCCATTCCCTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACATTAGCCATATTagag                             <  2:2244689/150‑1 (MQ=255)

                                                                                                                            tAATACCCATTCCCTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACATTAGCCATATTAGAGTCgg                         <  1:3477516/150‑1 (MQ=255)

                                                                                                                              aTACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACATTAGCCATATTAGAGTCGGTa                       >  1:1869425/1‑150 (MQ=255)

                                                                                                                              aTACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACATTAGCCATATTAGAGTCGGTa                       <  1:1821392/150‑1 (MQ=255)

                                                                                                                                 cccATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACATTAGCCATATTAGAGTCGGTACat                    <  2:3153052/150‑1 (MQ=255)

                                                                                                                                 cccATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACATTAGCCATATTAGAGTCGGTACat                    <  2:3155643/150‑1 (MQ=255)

                                                                                                                                  ccATTCCCTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACATTAGCCATATTAGAGTCGGTACata                   <  2:3618998/150‑1 (MQ=255)

                                                                                                                                  ccATTCCCTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACATTAGCCATATTAGAGTCGGTACata                   >  1:2571768/1‑150 (MQ=255)

                                                                                                                                    aTTCCCTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACATTAGCCATATTAGAGTCGGTACAtata                 <  1:3625194/150‑1 (MQ=255)

                                                                                                                                    aTTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACATTAGCCATATTAGAGTCGGTACAtata                 >  1:1533352/1‑150 (MQ=255)

                                                                                                                                      tCCCTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACATTAGCCATATTAGAGTCGGTACATATAAc               >  1:1356839/1‑150 (MQ=255)

                                                                                                                                       cccTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACATTAGCCATATTAGAGTCGGTACATATAAcc              <  1:940470/150‑1 (MQ=255)

                                                                                                                                        ccTGGACGCTAGCTTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACATTAGCCATATTAGAGTCGGTACATATAACCa             <  1:3960530/150‑1 (MQ=255)

                                                                                                                                           ggACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACATTAGCCATATTAGAGTCGGTACATATAACCAttt          <  2:1293962/150‑1 (MQ=255)

                                                                                                                                            gACGCTAGCGTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACATTAGCCATATTAGAGTCGGTACATATAACCATTTc         <  2:1373690/150‑1 (MQ=255)

                                                                                                                                            gACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACATTAGCCATATTAGAGTCGGTACATATAACCATTTc         <  2:751596/150‑1 (MQ=255)

                                                                                                                                                  aGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACATTAGCCATATTAGAGTCGGTACATATAACCATTTCGAGGaa   >  2:3474153/1‑150 (MQ=255)

                                                                                                                                                  aGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACATTAGCCATATTAGAGTCGGTACATATAACCATTTCGAGGaa   >  1:3127231/1‑150 (MQ=255)

                                                                                                                                                   gCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACATTAGCCATATTAGAGTCGGTACATATAACCATTTCGAGGAAc  <  1:584618/150‑1 (MQ=255)

GCATGAAGATTATCCTCAGTTTGTGACATGCCAGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACATTAGCCATATTAGAGTCGGTACATATAACCATTTCGAGGAAC  >  CP009273/4271757‑4272053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A