Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F9 I0 R1	105	139.1	2894862	97.5%	2822490	224.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,151,328	T→G	19.2%	A590A (GCA→GCC)	ppc ←	phosphoenolpyruvate carboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,151,328	0	T	G	19.2%	140.1 / 2.1	96	A590A (GCA→GCC)	ppc	phosphoenolpyruvate carboxylase
Reads supporting (aligned to +/- strand): ref base T (51/16); new base G (13/10); total (67/29)
Fisher's exact test for biased strand distribution p-value = 1.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTAGACATCGCAGGAGATGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGC‑GACAGGC‑TGCTGACGGTGATTTCTGGC‑AGACCATATTTAAAGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGAGCAGCTGGGTCATGACATCGTTGGCG  >  NC_000913/4151093‑4151559

ggTAGACATCGCAGGAGATGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGC‑GACAGGC‑TGCTGACGGTGATTTCTGGC‑AGACCATATTTAAAGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTgc                                                                                                                                                                                                                                         >  1:827207/1‑238 (MQ=255)

 gTAGACATCGCAGGAGATGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGC‑GACAGGC‑TGCTGACGGTGATTTCTGGC‑AGACCATATTTAAAGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTTGGAACGAAGCGCCGCAAGTGCAGGTgcc                                                                                                                                                                                                                                        >  1:786246/1‑237 (MQ=255)

  tAGACATCGCAGGAGATGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGC‑GACAGGC‑TGCTGACGGTGATTTCTGGC‑AGACCATATTTAAAGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGAGcgcc                                                                                                                                                                                                                                      >  1:751241/1‑239 (MQ=255)

          gCAGGAGATGACTTACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGC‑GACAGGC‑TGCTGACGGTGATTTCTGGC‑AGACCATATTTAAAGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGATAGCAACGCCGCATGAGCCGGGGCGGCGCCGCGa                                                                                                                                                                                                                               >  1:717219/1‑238 (MQ=255)

                   gactgacAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGC‑GACAGGC‑TGCTGACGGTGATTTCTGGC‑AGACCATATTTAAAGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCCTGAGCAGGGGCGCCGCCGCGGCCAATGGaa                                                                                                                                                                                                                      >  1:30746/1‑238 (MQ=255)

                    actgacAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGC‑GACAGGC‑TGCTGACGGTGATTTCTGGC‑AGACCATATTTAAAGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGAAAGCAGCGCCGCATGAGCAGGTTCGCCGCCGCGGCCAATgggag                                                                                                                                                                                                                     >  2:966335/1‑235 (MQ=255)

                        acAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGC‑GACAGGC‑TGCTGACGGTGATTTCTGGC‑AGACCATATTTAAAGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGGCAACAGGCCCGCATGAGCCGGCTggccgcccgtctctttttaacacctg                                                                                                                                                                                                                >  2:238310/1‑220 (MQ=255)

                        acAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGAGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGC‑GACAGGC‑GGCTGACGGTGATTTCTGGC‑AGCCCATATTTAACGCGGATCAT‑CTCGCCCTGTTCGGTTAAGCGCAGGCCGCCTTTCAGGCTTCCTTGC‑GG‑GTTGGACAGCAGCGCCGCCTGCGAGGGGGggccgcccgggcccacgggaacgac                                                                                                                                                                                                                 >  2:1064714/1‑220 (MQ=255)

                              cATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGC‑GGCAGGC‑TGCTGACGGAGATTTCTGGC‑AGACCATATTTAAAACGGATCAT‑CTCGCCCTGTTCGGTTACGCTCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑GTGTTAACGCAGCGCACCCGGAGGCGGGGCGCGCCCGCCACCAATGGGA‑cacacccgcacg                                                                                                                                                                                                         >  2:198436/1‑228 (MQ=255)

                                      aTGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGC‑GACAGGC‑TGCTGACGGTGATTTCTGGC‑AGACCATATTTAAAGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGCCCAATGGAA‑CCCCCGCGACCGGGGaaaa                                                                                                                                                                                                  >  1:364406/1‑236 (MQ=255)

                                      aTGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGC‑GACAGGC‑TGCTGACGGTGATTTCTGGC‑AGACCATATTTAAAGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCCCGACCAATGGCA‑CCGCCGCGACCGTGGgaca                                                                                                                                                                                                  >  1:559832/1‑238 (MQ=255)

                                           aCGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGC‑GACAGGC‑TGCTGACGGTGATTTCTGGC‑AGACCATATTTAAAGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCa                                                                                                                                                                                            >  1:1316663/1‑239 (MQ=255)

                                           aCGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGC‑GACAGGC‑TGCTGACGGTGATTTCTGGC‑AGACCATATTTAAAGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCa                                                                                                                                                                                            >  1:1443478/1‑239 (MQ=255)

                                           aCGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGC‑GACAGGC‑TGCTGACGGTGATTTCTGGC‑AGACCATATTTAAAGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCa                                                                                                                                                                                            >  1:473689/1‑239 (MQ=255)

                                            cGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGAATCCAGAATCGCCCCGGTATAAAGC‑GACAGGC‑TGCTGACGGTGATTTCTGGC‑AGACCATATTTAAAGCGGATCAT‑CTCCAACTGTTCGGTTACGGGCAAGCCGCCTTTGCGGCTTCATGGCGGG‑TTGGGA‑AGCAGCCCCCGCTCGGCACGGACCGCGCCGCGACCAAACGAA‑CCCCCGCGGCCCATGaacaaccaca                                                                                                                                                                                            >  2:854294/1‑234 (MQ=255)

                                                             cTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGC‑GACAGGC‑TGCTGACGGTGATTTCTGGC‑AGACCATATTTAAAGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCGTGAGCCGGGGCGCCGCCGCGACCAATGGAA‑CCGCCCCGACCGCGGGACAACGGCACCTCAATCACCCCttttt                                                                                                                                                                          >  2:922522/1‑239 (MQ=255)

                                                               ccGGGGGGGGCGGGAGTTTGGTTTCCAGAACCGCCCGGATA‑AAAGCGGAGGGCC‑GGCGGTCGGT‑ATTTTTGGCAAGACAATTTTAAAAGCGGTTCTC‑CTCCCCTTGTGGGGTTCGGCGC‑GGCCCCCTTTTCGGCTTCCGGGG‑GGTTTGGGACAGCAGCGCCGCATGAGGGGGGGCCCCCCCGCGCCCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCg                                                                                                                                                                        <  2:307322/239‑1 (MQ=255)

                                                               ccGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGC‑GACAGGC‑TGCTGACGGTGATTTCTGGC‑AGACCATATTTAAAGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCGCCGCCGCATGAGCACGGGCGCCGCCCCGACCCCAGGAG‑CCGCGCCGACCCTGGATAACCTCCCACTCAATACCCGCCTTTTc                                                                                                                                                                         >  2:914159/1‑238 (MQ=255)

                                                                       ggCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGC‑GACAGGC‑TGCTGACGGTGATTTCTGGC‑AGACCATATTTAAAGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCCTGAGCAGGGGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGGGGAACAACGTCCGCTCAATACCCGCTTTTTCGCAGGtttt                                                                                                                                                                >  1:1093957/1‑239 (MQ=255)

                                                                       ggCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGC‑GACAGGC‑TGCTGACGGTGATTTCTGGC‑AGACCATATTTAAAGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGGGCGCCGCCGCGGCCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGtttt                                                                                                                                                                >  1:625162/1‑239 (MQ=255)

                                                                                    cctCCAGCACCCCCCCGGTATAAAGC‑G‑CAGGCGTGCGGGGGTTGTTTCTTGGC‑ACCCAATTTTTAAGGGTGACCAT‑CCCGCCCTGTCCGTTTCGGCGCGGCCGGCCTTTCGGCCTTCGGGGG‑GT‑GTGGGACGGCCGCGCCGCAGGAGCGGGTCCCCCGCCGGTCCCATTGAAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATc                                                                                                                                                   <  2:1345598/236‑1 (MQ=255)

                                                                                     tgtgtaaaaaagaaagttaaaaaGC‑GACGGGC‑TGCTGCCGGGTTTTTGTGGC‑GGACCATATTAAAGGCGGATCCC‑CGCGCTCTGTTGGGTTCCGCGCGGGCCGCCTTCCGGGCTCCCGGGG‑GG‑TTGGGACAGCGGCGCCGCATGAGGGGGCGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCt                                                                                                                                                 <  2:349416/219‑1 (MQ=255)

                                                                                                ccccGGTATAAAGC‑GACAGGC‑TGCTGACGGTGATTTCTGGC‑AGACCATATTTAAAGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGatat                                                                                                                                      >  1:22611/1‑239 (MQ=255)

                                                                                                  ccagTATAAAGC‑GCCAGGC‑GACTGGTGGTGTTCTGTGAC‑‑GAACATTTTTAAAGGTGAACCTCCCCCCCCTTTTCGTTAGCGCGCAGGCCCCTTGACAGGTTTCCTGGG‑GG‑TGGAGACAGCCGCGCCGCGGGGGCGGGGGCCCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTg                                                                                                                                    <  2:625162/236‑1 (MQ=255)

                                                                                                     gTATAAAGC‑GACAGGC‑TGCTGACGGTGATTTCTGGC‑AGACCATATTTAAAGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCCCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTtcg                                                                                                                                  >  1:218183/1‑238 (MQ=255)

                                                                                                           aGC‑GACAGGC‑TGCTGACGGTGATTTCTGGC‑AGACCATATTTAAAGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGGCAGCTCAATACCCGCTTTTTCCCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATCTTGCGCCCAGGa                                                                                                                           >  1:116323/1‑239 (MQ=255)

                                                                                                           aGC‑GACAGGC‑TGCTGACGGTGATTTCTGGC‑AGACCATATTTAAAGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCCCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGCCCGTGGAACAACCTCAGCTCAAAACCCCCTTTTTCCCAGGGTTTG‑ATTAATGCCTCCTGTGCCCGATATTGCACCCAGGa                                                                                                                           >  2:932283/1‑239 (MQ=255)

                                                                                                                aaggggttgctgACGGGTTTTCCGGGC‑AAACCGTTTTAAAGGCGGTTCGT‑CCCCCCTTGTCCGTTCACGCGCAGCCCCCCTTCCGGGCTTCCGGGC‑GG‑TGGTGACAGCCGCGCCGCATGGGCGGGTGCGCCCCCGCGACCAGTGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAg                                                                                                                         <  2:30746/231‑1 (MQ=255)

                                                                                                                aCAGGC‑TGCTGACGGTGATTTCTGGC‑AGACCATATTTAAAGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGaa                                                                                                                          >  1:878792/1‑236 (MQ=255)

                                                                                                                aCAGGC‑TGCTGACGGTGATTTCTGGC‑AGACCATATTTAAAGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGGTTTG‑ATTAATGCATCCCGTGCCTGATATTTCGCCCCGGaa                                                                                                                          >  2:580902/1‑236 (MQ=255)

                                                                                                                aCAGGC‑TGCTGACGGTGATTTCTGGC‑AGACCATATTTAAAGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGGGCGCCGCCGCGACCAATGGAA‑CCGCCGCGCCCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGaa                                                                                                                          >  1:708404/1‑236 (MQ=255)

                                                                                                                 cAGGC‑TGCTGACGGTGATTTCTGGC‑AGACCATATTTAAAGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGata                                                                                                                                       >  1:349416/1‑222 (MQ=255)

                                                                                                                 cAGGC‑TGCTGACGGTGATTTCTGGC‑AGACCATATTTAAAGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATAtt                                                                                                                                     >  1:448430/1‑224 (MQ=255)

                                                                                                                 cAGGC‑TGCTGACGGTGATTTCTGGC‑AGACCATATTTAAAGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCt                                                                                                                       >  1:736768/1‑238 (MQ=255)

                                                                                                                 cAGGC‑TGCTGACGGTGATTTCTGGC‑AGACCATATTTAAAGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCt                                                                                                                       >  1:112726/1‑238 (MQ=255)

                                                                                                                 cAGGC‑TGCTGACGGTGATTTCTGGC‑AGACCATATTTAAAGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCt                                                                                                                       >  1:111013/1‑238 (MQ=255)

                                                                                                                 cAGGC‑TGCTGACGGTGATTTCTGGC‑AGACCATATTTAAAGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCt                                                                                                                       >  1:317012/1‑238 (MQ=255)

                                                                                                                 cAGGC‑TGCTGACGGTGATTTCTGGC‑AGACCATATTTAAAGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGAAAGCAGCGCCGCACGAGCAGGGGAGCCGCCGCGACCAATGGAA‑CCGCCGCCACCGTGGAACAACGTCAGCTCAATACCCGCTTCTTCGCAGGTTTTG‑ATTAATGGATCCTGTGCCTGATATTGCCCCCCGGAAGCt                                                                                                                       >  2:389486/1‑238 (MQ=255)

                                                                                                                               gtgtttgccgggcgacccATTTTAAAGGGGGTTCCT‑CCCGCCCTTTTGGGTTACGCGCGGGCCGCTTTCCAGGCTCCCGGGG‑GT‑TTGGGACGGCCGCGCCGCAGGGGCAGGGGCCCCCCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTccc                                                                                                           <  2:1095715/222‑1 (MQ=255)

                                                                                                                                             gACCATATTTAAAGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTT‑ATTAATTCATCCTGTGCCTGCTATTTCGCCCAGGAAGCTGCCCTTCCCCCCCCCTCTTTTGCTGAg                                                                                            >  2:1355476/1‑239 (MQ=255)

                                                                                                                                             gACCATATTTAAAGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGc                                                                                                                                             >  1:488210/1‑190 (MQ=255)

                                                                                                                                              aCCATATTTAAAGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGGGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAg                                                                                            >  1:8377/1‑238 (MQ=255)

                                                                                                                                                        aaGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACCGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTTCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGcc                                                                                 >  1:1184929/1‑239 (MQ=255)

                                                                                                                                                        aaGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGGGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGGGTCGGAATAGcc                                                                                 >  1:30847/1‑239 (MQ=255)

                                                                                                                                                             gATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCCTCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAatca                                                                            >  1:626497/1‑239 (MQ=255)

                                                                                                                                                             gATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCATGAAGCTGCCATCACTCCCGCCTCTTTTGCTGAGTCGGGATAGCCAat                                                                              >  1:1058585/1‑237 (MQ=255)

                                                                                                                                                             gATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAatca                                                                            >  1:134020/1‑239 (MQ=255)

                                                                                                                                                             gATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCCTTTTCCCCGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAatca                                                                            >  2:1191452/1‑239 (MQ=255)

                                                                                                                                                             gATCAT‑CTCGCCCTGTTCGGCTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAACAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGCCCGTGGGACAACGTCACCTCAATACCCGCTCTTTCGCAGGTTTCG‑GTTAATGCATCCTGTGCCTTGTATTTCGCCCCGGCAGCTGCCATCACTCCCCCCTCTTTTGCtactcttggatagccaatca                                                                            >  2:67769/1‑220 (MQ=255)

                                                                                                                                                              atcat‑cTCGCCCTGTTCGGTTACGCTCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGATAGCAAGGCCGCAAGAGCCGGGGAGCCGCCCGGACCAATGGGA‑CCGCCGGGAACGTGGAACCACGTCAGCGCCATACCCGAGGGTTCGCAGGTTTTGTATTAA‑CCGTCACTCGTCTGGAATTTGGGCCCGGGAGCTGACATCCCTCCCGCCTCTTTTTGTGGGTCGGaacgtgcactcg                                                                            >  2:271861/1‑228 (MQ=255)

                                                                                                                                                              atcat‑cTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAatca                                                                            >  1:152461/1‑238 (MQ=255)

                                                                                                                                                               cccctcccGCCCTGTGCGGTCCCGCGCGGCCCGCCTTTCGCCTTTCCTGGC‑GG‑TTGCGACAGCAGCCCCGCAGGAGCGGGGGCCCCGCCGCGCCAAATGAAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAatcat                                                                           <  2:864942/232‑1 (MQ=255)

                                                                                                                                                                        cccTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCa                                                                      >  1:481799/1‑235 (MQ=255)

                                                                                                                                                                         ccggttgggtaccccgcgGGCCGCCTTTCGGGCTTCCGGGC‑GG‑TGTGGACAGCAGCGCCGCAGGGGGGGGTGCCCCCCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGt                                                                 <  1:454995/225‑1 (MQ=255)

                                                                                                                                                                          gtggccGGGTACGCGCGGCCCGGCTTGCGGGCTTGCGGGG‑GG‑TTGCGACAGCGCGGCCGCAGGAGAGGGGGCCCCGCCGCGACAAGTGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGtt                                                                <  2:1093957/234‑1 (MQ=255)

                                                                                                                                                                             ttCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTg                                                              >  1:761034/1‑238 (MQ=255)

                                                                                                                                                                              gcGGTTCCGGGCAGGCCCCTTTTCGGGCTTCGGGGG‑GG‑TTGGGACAGCGGCGCCGCAGGAGCAGGTGCGCCGCCGCGACAAGTGAAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGcc                                                            <  1:170593/238‑1 (MQ=255)

                                                                                                                                                                                  ttACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGTATCCTGTGCCTGATATTTCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGa                                                        >  1:121073/1‑239 (MQ=255)

                                                                                                                                                                                       cgcAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAg                                                   >  1:470745/1‑239 (MQ=255)

                                                                                                                                                                                          aGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCTCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGc                                                                                                                        >  1:712958/1‑167 (MQ=255)

                                                                                                                                                                                          aGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCTCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGc                                                                                                                        <  2:712958/167‑1 (MQ=255)

                                                                                                                                                                                            gccgccTTTCGGGCTTCCTGTG‑TG‑TTGTGGCAGCAGCGCCGCATGAGCGGGTGCGCCGCCGCGACCAATGGAC‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCa                                               <  1:330757/238‑1 (MQ=255)

                                                                                                                                                                                            gccgccTTCGGGGCTTCCGGGC‑GG‑TGGGGACGGCGCCGCCGCAGGAGCGGGTGCCCCGCCGCGACCAATGGAC‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCa                                               <  2:111013/238‑1 (MQ=255)

                                                                                                                                                                                            cccgccTTTCGGCTTTCCGGTG‑GG‑TTGTGACGGCGGCGCCGCTTGAGCGGGTGCGCCGCCGGGCCCAATGGAC‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCa                                               <  1:1401273/237‑1 (MQ=255)

                                                                                                                                                                                            cccccttttCGGGCTTCCGGGG‑GG‑TTGGAGCAGCGCCGCCGCATGAGCAGGTGCGCCGCCGCGACAAATGAAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCa                                               <  1:908337/232‑1 (MQ=255)

                                                                                                                                                                                                tcTTGCAGGCTGCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGCGCCCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATa                                          <  1:548995/238‑1 (MQ=255)

                                                                                                                                                                                                cttttCAGGCTCCCTGGC‑GG‑TTGTGACAGCGGCGCCGCATGAGCAGGGGCGCCGCCGCGACCAAGGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATa                                          <  1:1107527/237‑1 (MQ=255)

                                                                                                                                                                                                ccctccaggtcttcTGGG‑GT‑TTGTGACCGCCGCTCCTCATGACGGGGTGCACCCCCGACACGTATGAAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATa                                          <  2:1443478/226‑1 (MQ=255)

                                                                                                                                                                                                ccTTTCGGGCGGCCTGGC‑GG‑TTGTGCCAGGAGGGCCGCATGAGCGGGGGCGCCGCCCAGCCAGATGAAC‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATa                                          <  2:152461/239‑1 (MQ=255)

                                                                                                                                                                                                 cTTTCGGGCTCCCGGGG‑GG‑TTGGGACAGCAGCGCCGCAGGAGCAGGGGCGCCGCCGCGACAAAGGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATAc                                         <  1:401094/239‑1 (MQ=255)

                                                                                                                                                                                                    ttccgggctccgggggggtggggACAGCCGCGCGCCAGGAGCAGGTGCCCCCCCGCGCCCATTGAAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATAcc                                        <  2:157449/217‑1 (MQ=255)

                                                                                                                                                                                                     ttggCTTCCGGGC‑GT‑TTGTGACGGCGGCCCCGCATGGGCAGGGGCCCCGCC‑TGACCCATGGAATCCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑AGTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGt                                     <  1:854294/237‑1 (MQ=255)

                                                                                                                                                                                                     cgggtttCCTGGG‑GG‑TGGGGACAGCGCCGCCGCAGGGCCGGGGGCGCCGCGGCGACAAATGAAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGt                                     <  1:580902/234‑1 (MQ=255)

                                                                                                                                                                                                     cAGCCTTCGGGGG‑GG‑TTGTGACAGCACCGCCGCATGAGCAGGTGCGCCCCCGCAACCAAGGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGt                                     <  1:562830/239‑1 (MQ=255)

                                                                                                                                                                                                      aGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCCGGGTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGCAGCTGCCATCACTCCCCCATCTTTTGCTGAGTCGGGATAGCAAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTc                                    >  2:237316/1‑239 (MQ=255)

                                                                                                                                                                                                        gCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCaa                                  >  2:900582/1‑239 (MQ=255)

                                                                                                                                                                                                          ttCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCaa                                  >  2:963993/1‑237 (MQ=255)

                                                                                                                                                                                                          ttCCGGGG‑GG‑TTGTGACAGCAGCGCCGCATGAGCGGGTGCGCCGCCGCGACCAAGGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATa                                          <  1:360548/229‑1 (MQ=255)

                                                                                                                                                                                                           tCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGGGCCGGTGCGCCGCCGCGACCAATGGCA‑CCGCCGCGACCGTGGGACCACGTCAGCTCCATACCCGCTTTTTTCCAGGGTTTG‑ATTAATGCATCCTGTGCCCGTTATTGCGCCCAGGAAGCTGCCCTCCCTCCCGCATCTTTTGCTGCGTCGGCATAGCCAATCATCCCCATCTGTTTGCCCTGAATCCGGCCACGCTACCCGTCAatat                               >  2:257231/1‑239 (MQ=255)

                                                                                                                                                                                                           tCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCCTCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGGATCCGGCCCCGATACCAGTCAatat                               >  1:271970/1‑239 (MQ=255)

                                                                                                                                                                                                           tCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAatat                               >  1:157449/1‑239 (MQ=255)

                                                                                                                                                                                                           tCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAatat                               >  1:635949/1‑239 (MQ=255)

                                                                                                                                                                                                           tCCTGGC‑GG‑TTGTGACAGCAGCGCCCGATGAGCAGGGGCGCCGCCGCGACCAATGGAA‑ACGCCGCGCCCGTGGAACAACGTCAGCTCACTACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCCGATATTGCGCCCCGGAAGCTGCCATCCCTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATTCTCACCCTCCGTTTCCCCTTCACCAGGCCACGATACCAGTCCatat                               >  2:992459/1‑239 (MQ=255)

                                                                                                                                                                                                            ccTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATAGCAGTCAATAtt                              >  1:896599/1‑239 (MQ=255)

                                                                                                                                                                                                                gC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGag                           <  1:963993/238‑1 (MQ=255)

                                                                                                                                                                                                                      ttGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCCCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTTCGCCCCGGAAGCTGCCATCACTCCCCCATCTTTTGCTGAGTCGGGATAGCCAATCATCACCATCTTTTTGCCCTGAATCAGGCCCCGATAACAGTCAata                                >  2:360548/1‑229 (MQ=255)

                                                                                                                                                                                                                      tgtgaacggcagcGCCGCAGGGGAAGGCGCGCCCCCGCACCAAAGGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGagcagc                       <  1:544626/230‑1 (MQ=255)

                                                                                                                                                                                                                       tgtgACAGCAGCGCCGCATGAGCAGGAGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCCCCTCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTTAATCAGGCCCCGACACCAGTCAATATTGAGCAGCTg                     >  2:842251/1‑239 (MQ=255)

                                                                                                                                                                                                                        gtgACAGCCGCGCCGCCTTAGCAGGTGCGGCGCCGCGCCCAATGGAA‑CCGCCGCGCCCGTGGAACAACGTCAGCTCAATACCCCCTTTTTCGCAGGCTTTG‑ATTAATGCATCCTGTGCCCGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCCTTTGCCGCTTCGGCCTAGCCAATCATCAACATCTGTTTGCCCTGAATCAGGCCCCGATACCAGTAAATAtttcaccgcttg                    >  2:544626/1‑229 (MQ=255)

                                                                                                                                                                                                                              gcagcGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGAGCAGCTGGGTCATg              >  1:244051/1‑239 (MQ=255)

                                                                                                                                                                                                                                agcGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGGCAATATTGAGCAGCTGGGTCATGAc            >  2:1423406/1‑239 (MQ=255)

                                                                                                                                                                                                                                     cgcATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGAGCAGCTGGGTCATGACATCGt       >  1:1426610/1‑239 (MQ=255)

                                                                                                                                                                                                                                       cATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGAGCAGCTGGGTCATGACATCGTTg     >  2:578458/1‑239 (MQ=255)

                                                                                                                                                                                                                                           ggcgggTGCCCCCCGGCGCCCAAGGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGAGCAGCTGGGTCATGACATCGTTGGCg  <  2:878792/234‑1 (MQ=255)

                                                                                                                                                                                                                                               ggTGCGCCGCCGCGACCTATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATg                                                                                                                                                       <  2:1291157/85‑1 (MQ=255)

                                                                                                                                                                                                                                               ggTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATg                                                                                                                                                       >  1:1291157/1‑85 (MQ=255)

GGTAGACATCGCAGGAGATGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGC‑GACAGGC‑TGCTGACGGTGATTTCTGGC‑AGACCATATTTAAAGCGGATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGC‑GG‑TTGTGACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAA‑CCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTG‑ATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGAGCAGCTGGGTCATGACATCGTTGGCG  >  NC_000913/4151093‑4151559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A