Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F9 I0 R1 71 67.1 1514203 90.7% 1373382 220.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,609,768 T→G 24.0% G118G (GGT→GGG)  prfC → peptide chain release factor RF‑3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,609,7680TG24.0% 102.4 / 8.0 67G118G (GGT→GGG) prfCpeptide chain release factor RF‑3
Reads supporting (aligned to +/- strand):  ref base T (18/27);  new base G (12/6);  total (34/33)
Fisher's exact test for biased strand distribution p-value = 9.27e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAAAAGGCCGTGGTTCCAACCAGCACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAG‑GTGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTACCACCTTTATAAAGACGAAACCTAT  >  NC_000913/4609551‑4609987
                                                                                                                                                                                                                          |                                                                                                                                                                                                                            
tataagggacaggTTTCAACCCGCCAGCTAAGGTGGACTGGGTGGAGATGGAAAAGCAGCGTGGGAGCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACCCCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAG‑GTGTTGAAGATCg                                                                                                                                                                                                                   <  2:596292/218‑1 (MQ=255)
tAAAAGGCCGTGGTTCCAACCAGCACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATTACTCCGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAG‑GTGTTGAAGATCg                                                                                                                                                                                                                   <  1:136413/229‑1 (MQ=255)
  aaaGGCCGTTTTTCCGACCAGCCCGCTAAAGTGGACTTGGTGGAGATGGAAAGGCAGCGTGGGATTTCCCTTACGACGTCTGTGGTGCAGTTTCCGTATCACGATTGCCTGGTTACCCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAG‑GTGTTGAAGATCG‑Ta                                                                                                                                                                                                                <  2:283485/229‑1 (MQ=255)
       ccGTGGTTCCAACCAGCACGTTAAGTCGTACTGGATGGACATGGAAAAGCAGCGTGGGATCGCCATTACTACGTCTGTGATGCAGGTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTTCCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAG‑GTGTTGAAGATCG‑TACCCGt                                                                                                                                                                                                           <  1:591501/229‑1 (MQ=255)
          tGGTTCCAACCAGCACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATTGCTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGTCACCCCGGGGCACGAAGACTTCTCGGAAGATACCAATCGTACCCTGACGGCGGCGGGATGCGGCCTGAGGGGTCTCGCAGAACCAAAAG‑GAGTGGAAggcttgtctcttata                                                                                                                                                                                                         >  2:72481/1‑215 (MQ=255)
          tGGTTCCAACCAGCACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACCGCTGCCTGGTGGTTTTCGACCCCCCAAACG‑GGGTTggaacacttacccctaaa                                                                                                                                                                                                         >  2:719952/1‑212 (MQ=255)
                 aaccgtgaCGCTAAGTCGGACTGGATGGATATGGAAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAG‑GTGTTGAAGATCG‑TACCCGTAAGCTGATg                                                                                                                                                                                                  <  1:550607/221‑1 (MQ=255)
                       cACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGCCTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGCCGCGCCACAAG‑GAGTTTGAGCTCG‑TACCCGACACTTGATGGAGGtt                                                                                                                                                                                            >  1:735742/1‑228 (MQ=255)
                       cACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATGGACGCGCCACAAG‑GGGTTGAAG‑TCGTTACCCGTAAGCTGATGGAAGtt                                                                                                                                                                                            >  1:544450/1‑228 (MQ=255)
                       cACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGGGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAG‑GGGTTGAAGATCG‑TACCCGGAAGcccgcctcttata                                                                                                                                                                                           >  1:596293/1‑217 (MQ=255)
                       cACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGGGGACGTCTGCCCGAGGGTTATCGACGCC‑CACAAGAGGGGTGAAAACCG‑TACCCGTAAggttgtggagagaa                                                                                                                                                                                           >  2:490329/1‑216 (MQ=255)
                                                gggaAAAGCGGCGTGGGGTCTCCATTCTTCCTTGTGTGCTGCATTTTCCGATTCCCGATGGCCTGGTTAACCTGCCCGACCCCCCGGGGGACGAAGACTTCTGGGAAGATACCTACCGTCCCCGGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAG‑GTGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGTTCCCCGTCTGCGCGACACGCCGATCCt                                                                                                                                                                  <  2:285674/228‑1 (MQ=255)
                                                  gaaaaacgacttGGGTTCTCCTTTCCTCCGTTGTTGATGCATTTCTCGTATCCGGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTTCCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAG‑GTGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCa                                                                                                                                                                <  2:446809/218‑1 (MQ=255)
                                                  gaAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAG‑GTGTTGAAGATCG‑TACCCGTAAGCTGATTGAAGTTACCCGTCTGCGTGACACGCCGATCCTCa                                                                                                                                                                >  1:72006/1‑229 (MQ=255)
                                                  gaAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAG‑GGGTTGAAGATCG‑TACCCGTAAGCTGCTGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCa                                                                                                                                                                >  1:559326/1‑229 (MQ=255)
                                                  gaAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGCGGACTGCTGCCCGATGGTTATCGACGCCGCAAAAG‑GTGTTGAAACTCG‑TACCCGTAAGCTGATGGAAGTTACCCGTCCGCGCGACAGCCCGctcctc                                                                                                                                                                 >  1:219010/1‑228 (MQ=255)
                                                        catgtggggATCTCCATTACTAGGTCGGTGATGCGGTTTCCGTATCCCGATTGCCTGGTTAACCTGCTCAGCACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAG‑GTGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCttt                                                                                                                                                           <  2:153030/222‑1 (MQ=255)
                                                        cagcGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAG‑GGGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTa                                                                                                                                                          >  1:17218/1‑229 (MQ=255)
                                                            gtgggattctcttttcttcctCTGTGAAGGTGTTTCAGTAACCCTGTTGCCCGGTTACCCTGCTCGACTCCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGGGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAG‑GTGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCCCCTTTATGaa                                                                                                                                                      <  2:391648/209‑1 (MQ=255)
                                                             tGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAG‑GTGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGTTACCCGCCTGCGCGACACCCCGATCCTCACCTTTATGAAc                                                                                                                                                     >  1:10550/1‑229 (MQ=255)
                                                                     ccATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGCCTTCTCGGCAGATACCTATCGTACCCTGACGGCGGCGGACTGCTGCCTGATGGTTATCGCAGCCGCCAAAG‑GGGGTGAAGCTCG‑TACCCGTAAGCTGATGGAAGTTACCCTGCTGCACGCCACGCCCGTCCTCACCCTTATGAAAAAAATTg                                                                                                                                              >  2:728503/1‑228 (MQ=255)
                                                                      ctgtactgcGTCTGGGATGCCGTTTCCGTATCACGATTGCCTGGTTTACCTGCTCGACGCCCCGGGGCACGAAGTCTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCGGCCTGATGGTTATCGACGCCGCAAAAG‑GTGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTg                                                                                                                                              <  1:222458/224‑1 (MQ=255)
                                                                      cATTACTCCGTCTGGGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAG‑GTGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGTCACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACt                                                                                                                                                <  1:299396/225‑1 (MQ=255)
                                                                       cttctcacctgtgtgAGGCAGTTTCCGTTACTCAATTGCCCGGTTAACCGGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTGTCGTACCCGGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAG‑GTGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGAc                                                                                                                                            <  2:207017/217‑1 (MQ=255)
                                                                              cGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAACAG‑GGGTTGAAGATCG‑TAACCGTAAGCTGATGGAAGTTAACCGTCTGCTCGAGACGCCGATCCTAACCTTTATGAACAAACTTGCCCGTGgta                                                                                                                                     >  1:731343/1‑228 (MQ=255)
                                                                              cGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAG‑GTGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGGGatat                                                                                                                                    >  1:119019/1‑229 (MQ=255)
                                                                                tCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAG‑GTGTTGAAGATCG‑TACCCGGAAGCTGATGGCAGTTACCCGCCTGCGCGACACGCCGGTCCTCACCTTTATTAACAACTTTAACCGTGatattc                                                                                                                                  >  1:600467/1‑227 (MQ=255)
                                                                                tCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAG‑GAGTTGAAAATCG‑TACCCGTAAGCTGGTGGAGGTTACCCGTCTGCGGGACAGGCCGATCCCAACCTTTATTAACAAACTTGACCGGGTTATcc                                                                                                                                  >  1:91849/1‑229 (MQ=255)
                                                                                tCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCAGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATTGACCACCCAAAAG‑GGGTTGAGGATCG‑TACCCGTGAGCTGATGGAAGTTAACCGCGTGCGGGCAATGCCGATCCTCACCCTTATGCAAAAAATTGACCggagtatcc                                                                                                                                  >  2:37261/1‑221 (MQ=255)
                                                                                      aggaatgttCCGTATCGCGATGGGCTGGTTAACCTGCCGGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGGGGTGGACTGCTGCCTGAGGGTTATCGACGCCGCAAAAG‑GTGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGTTCCCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGAcc                                                                                                                            <  2:219010/222‑1 (MQ=255)
                                                                                                cGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAG‑GGGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAgct                                                                                                                  >  1:377606/1‑229 (MQ=255)
                                                                                                 gTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAG‑GTGTTg                                                                                                                                                                                                                          <  1:158055/125‑1 (MQ=255)
                                                                                                    tCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGGGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAG‑GGGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGGCAAGCCGATCCTCACCCTTATGACCAAACTTTACCGCGTAATCCCCGAACCCATgaaccgtacg                                                                                                              >  2:709121/1‑220 (MQ=255)
                                                                                                                  tgaaccgtcTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATGCCTATCGTACCCTTACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAG‑GTGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGaa                                                                                                <  1:734428/221‑1 (MQ=255)
                                                                                                                           tCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAG‑GTGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGCTATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTgca                                                                                       >  1:515499/1‑227 (MQ=255)
                                                                                                                           tCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAG‑GTGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCTACCCGATGGAGCTGATCGATGAAGTTGAGAACCAGCTgca                                                                                       >  1:614689/1‑227 (MQ=255)
                                                                                                                           tCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAG‑GTGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGaa                                                                                       >  1:534711/1‑229 (MQ=255)
                                                                                                                              accccccGGGGCGCGAAGGCTTCTGGGAAGATACCCAAGGTATCCGGGCGGCGGTGGCCTGTGGTCTGATGGTTACCGAGGCCGCAAAAG‑TGGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCCCCTTTATGAACAAACTTGACCGTGATAGCCGCTACCCGATGGAGCTGCTCGATGAAGTTGAGCACGAGCTGAAAAt                                                                                    <  2:665280/226‑1 (MQ=255)
                                                                                                                                 ccgcGGGGCACGAAGACTTCTCGGAAGATACCGTTCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTGATCGACGCCGCAAAAG‑GTGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCgg                                                                                 <  1:360074/226‑1 (MQ=255)
                                                                                                                                 ccccGGGGCCAGGAGACTTCTCGGAAGATACCTAGCGTACCCTGACGGCGGTGGACTGATGCCTGATGGTTATCGACGCCGCAAAAG‑GTGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCgg                                                                                 <  1:623549/229‑1 (MQ=255)
                                                                                                                                 ccccGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAG‑GGGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGGTACCCGTCTGCGCGACACGCCGATCCTCACCTTTTTGAACAAACTTTACCGCGATATACCCGCCCCGATGGAGCTGCCCGGTGAGGTTggaaaaggacgtaaaaaca                                                                                  >  2:377815/1‑210 (MQ=255)
                                                                                                                                   ctggggCACGTGAACTTCCCGGAAGTTCCCTATCGTGCCCGGACGGCGGGGGCCTGCCGCCTGATGGTTATCGACGCCGCAAAAG‑GTGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGTTCCCCGTCTGCGCGACCCGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCt                                                                               <  2:158391/227‑1 (MQ=255)
                                                                                                                                    cGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAG‑GTGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGTTACCCGCCTTCGCGACACGCCGATCCTCACCTTTTTGAAGAAAATTAGCCCGGCTATCCCCCACCCGATGAGGCGGCTCGTGGACGGTCAGAACGAAATGAAAATCGTCtg                                                                              >  2:307303/1‑229 (MQ=255)
                                                                                                                                       ggCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAG‑GTGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGGGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGGAACCGAGCTGAAAATCGGCtgt                                                                             >  1:468361/1‑227 (MQ=255)
                                                                                                                                            gAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGAAGGTTATCGACGCCGCAAAAG‑GTGTTGAAGATCG‑TACCCGTAAGCTGATGGACGTTACCCGTCTGCGCGACACGCCGACCCTCACCTTTATGAACAAACTTGACCGGGATATCCCCGACCCGCTGGAGCTGCCCGAGTAATTTGGAAACGAGCTCAAAACCGGcgtggcgcgga                                                                      >  2:623548/1‑219 (MQ=255)
                                                                                                                                                   tctcGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAG‑GAGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGGTACCCGTCTGCGCGACACGCCGTTCCTCACCTTTATGAACAAACTTGCCCGGGCTAGCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGACCGACCTGGAAATCGGCACGGCGCCGATAACCTg                                                               >  2:198121/1‑229 (MQ=255)
                                                                                                                                                           gATACCTATCGTCCCCTGACGGCGGGGGACTGCCGCCTGATGGTTATCGACGCCGCAAAAG‑GTGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGAtt                                                        <  1:167866/228‑1 (MQ=255)
                                                                                                                                                                   tCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAG‑GTGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCCCCTGGCCGATTGCGTGCGGc                                               >  1:560602/1‑229 (MQ=255)
                                                                                                                                                                        cccGGACGGGGGTGAGCTGCGGCTTGATGGTTAGCGACCCCGCAAAAG‑TGGTTGAAGATGG‑TACCGTTAAGCGTGTGGAAGTTCCCCGTCTGCGCGACCCGCCGATCCTCACCGTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAg                                            <  2:618836/227‑1 (MQ=255)
                                                                                                                                                                           tGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAG‑GTGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCCCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCACGCTGtt                                       >  1:391763/1‑229 (MQ=255)
                                                                                                                                                                             gcggcggTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAG‑GTGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTa                                     <  1:490329/228‑1 (MQ=255)
                                                                                                                                                                             acgggggggtgatgcgtccTGATGGTTATCCACGTCGCAAAAG‑GTTGTTAAGATCG‑TCCCCGTAAGCTGATGGAAGTTTCCCGGCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTa                                     <  2:468361/212‑1 (MQ=255)
                                                                                                                                                                             aCGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAG‑GTGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAg                                            >  1:618837/1‑222 (MQ=255)
                                                                                                                                                                             aCGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCCCAAAAG‑GTGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACGAACTTGACCGTGATATCCGCGACCCGCTGGAGCTGCTCGATGAAGTTGAGAACGGGCTGAAAAACGGCTGTGGACCGGTCCCCTGGCCGGTTGGCTCGGGCAACCTGGTTa                                     >  2:172566/1‑229 (MQ=255)
                                                                                                                                                                                gcggTGGACTGCTGCCTGATGGTTATCGCCGCCGCACAAG‑GTGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGACATCCGCGACCCGATCGAGCTCCTCGATGAAGTTGAGAACCGGCGCAAAACCGGCTGTGCGCCGACCCCCCTGCCGATTGGCCGGGGCAACCTTTTTaaa                                   >  2:273199/1‑228 (MQ=255)
                                                                                                                                                                                        cgtctgcCTAATGGTTAGCGGCGCCGCAAAAG‑GGGTTGAAGATCC‑TCCCCGTAAGCTGATGGAAGTTACCCGGCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTac                          <  1:37261/226‑1 (MQ=255)
                                                                                                                                                                                         tgctgcCTGATGGTTATCGGCCCCGCAAAAG‑TTTTTGAACATCG‑TACCCTTAAGCTGATGGAAGTTCCCCGTCTGCGCGACCCGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTTTTTAAAGGCGTTTac                          <  1:342411/228‑1 (MQ=255)
                                                                                                                                                                                                   tGGTTTACGACGCCACAAAAG‑TGTTTGAACACGG‑GTCCCGTAAGCTGGTGGAAGTTCCCCGTCGGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTACCACCTTtata                <  2:391763/228‑1 (MQ=255)
                                                                                                                                                                                                       taacgccgccgcAAAAG‑GTGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGGCGATCCTCCCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTACCACCTTTATAAAGa            <  1:203084/222‑1 (MQ=255)
                                                                                                                                                                                                       tATCGACGCCGCAAAAG‑GTGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGCCGTTTACCACCTTTATAAAGAc           >  1:455166/1‑229 (MQ=255)
                                                                                                                                                                                                            acgtcccAAAAG‑GTTTTGAAGCTCG‑TACCCGTAAGCGGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTACCACCTTTATAAAGACGAAAc      <  1:709122/223‑1 (MQ=255)
                                                                                                                                                                                                              gccgcAAAAG‑GGGTTGAAGTTCG‑TACCCGTAGGCTGATGGAAGTTCCCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATACCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTACCACCTTTATAAAGACGAAACCt    <  2:515499/229‑1 (MQ=255)
                                                                                                                                                                                                              gccgcAAAAG‑GGGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGTTCCCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTACCACCTTTATAAAGACGAAACCt    <  1:719953/229‑1 (MQ=255)
                                                                                                                                                                                                              cccgcAAATG‑GTGTGGAAGCTCG‑TCCCCGTAATCTGATGGAAGTTACCCGTCTGCGCGACAGGCCGATCCTCCCCTTTATGAACAAACTTGACCGTTATAGCCGCGACCCGAGGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAACTCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTACCACCTTTATAAAGACGAAACCt    <  2:72006/228‑1 (MQ=255)
                                                                                                                                                                                                                cgcAAAAG‑GTGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGACAAAACTTGACCGTGATATCCGCGa                                                                                                                              >  2:76761/1‑105 (MQ=255)
                                                                                                                                                                                                                cgcAAAAG‑GTGTGGAAGATCG‑TACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGa                                                                                                                              <  1:76761/105‑1 (MQ=255)
                                                                                                                                                                                                                cccAAAAG‑GTGTTGAAGACCG‑TACCCGTAAGTTTAGGGAATTTCCCCGTCTGGGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTACCACCTTTATAAAGACGAAACCTAt  <  2:119019/227‑1 (MQ=255)
                                                                                                                                                                                                                          |                                                                                                                                                                                                                            
TAAAAGGCCGTGGTTCCAACCAGCACGCTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATTACTACGTCTGTGATGCAGTTTCCGTATCACGATTGCCTGGTTAACCTGCTCGACACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAG‑GTGTTGAAGATCG‑TACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTACCACCTTTATAAAGACGAAACCTAT  >  NC_000913/4609551‑4609987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A