Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A23 F20 I1 R1	21	53.9	1121892	93.4%	1047847	149.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,105,994	T→C	T12T (ACT→ACC)	pdhD →	dihydrolipoyl dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,105,994	0	T	C	100.0%	133.4 / NA	39	T12T (ACT→ACC)	pdhD	dihydrolipoyl dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/17); total (22/17)

TAAGCTTTGACCACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGCATTACTACATG  >  CP000730/1105852‑1106139

tAAGCTTTGACCACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTa                                                                                                                                              >  1:702171/1‑149 (MQ=255)

       tggccATAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAg                                                                                                                                       <  1:941137/146‑1 (MQ=255)

       tGACCACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATCGTAATCgggg                                                                                                                                       >  1:999761/1‑147 (MQ=255)

       tGACCACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAg                                                                                                                                       <  1:462849/149‑1 (MQ=255)

         aCCACATACAAATTGATGGTGCAACTGGCCAACATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑Ca                                                                                                                                    <  1:443027/149‑1 (MQ=255)

          ccACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAg                                                                                                                                   >  1:344706/1‑149 (MQ=255)

                     ttGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGCG‑CAGGTCCTGGTGGa                                                                                                                        >  1:307150/1‑149 (MQ=255)

                           gTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGtt                                                                                                                  <  1:348646/149‑1 (MQ=255)

                            tGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATACAAATTTAGTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTg                                                                                                                 >  1:771593/1‑149 (MQ=255)

                              cAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTgca                                                                                                               >  1:252137/1‑149 (MQ=255)

                                      cAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCg                                                                                                       <  1:782876/149‑1 (MQ=255)

                                         aaTGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGc                                                                                                    >  1:474947/1‑149 (MQ=255)

                                          aTGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCa                                                                                                   >  1:134043/1‑149 (MQ=255)

                                           tGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAg                                                                                                  <  1:603965/149‑1 (MQ=255)

                                             gaaTGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCt                                                                                                <  1:499761/148‑1 (MQ=255)

                                             cAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCt                                                                                                <  1:1022478/149‑1 (MQ=255)

                                                  aaTCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTACTCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCCATTCGTGCAGCTCAAtt                                                                                           >  1:210142/1‑149 (MQ=255)

                                                    tCACATTAAACGTTTATTAAATAATCCAGAATTATTATTACTGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAg                                                                                         >  1:767977/1‑149 (MQ=255)

                                                                     taAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATc                                                                        >  1:1057138/1‑149 (MQ=255)

                                                                                         ttaATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGGTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGGACTCTtgg                                                    >  1:178496/1‑149 (MQ=255)

                                                                                              ggAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGgtggtg                                               <  1:978765/149‑1 (MQ=255)

                                                                                               gAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCCATTAATTCATCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGgtgt                                              >  1:1098334/1‑149 (MQ=255)

                                                                                               gAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGgtgt                                              >  1:20581/1‑149 (MQ=255)

                                                                                                 ggggTAAAACATGGTAGTTGGAGATTTCCGAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGttt                                            >  1:587954/1‑149 (MQ=255)

                                                                                                 ggggTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGttt                                            >  1:230919/1‑149 (MQ=255)

                                                                                                   ggTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGc                                          <  1:1059643/149‑1 (MQ=255)

                                                                                                    gTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCt                                         <  1:35659/149‑1 (MQ=255)

                                                                                                             ggTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAg                                >  1:655997/1‑149 (MQ=255)

                                                                                                              gTAGTTTGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAgg                               <  1:1026167/149‑1 (MQ=255)

                                                                                                              gTAGTTGGAGATTTCCCAATGGAAACAGATCCCATAGTAATCGGAG‑CAGGCCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAgg                               <  1:515181/149‑1 (MQ=255)

                                                                                                               tAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGa                              >  1:1095749/1‑149 (MQ=255)

                                                                                                                aGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGAt                             <  1:187903/149‑1 (MQ=255)

                                                                                                                           tCCCAATTGAAACAGATACCATAGTACTAGGAGTCAGG‑CCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGGTGTATTCCTTCa                  >  1:547552/1‑149 (MQ=255)

                                                                                                                             ccgaTTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCaaa                <  1:418356/146‑1 (MQ=255)

                                                                                                                             ccAATTGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCaaa                >  1:827489/1‑149 (MQ=255)

                                                                                                                                agttaaaCAGATACCAAAGGAAACGGAG‑CAGGGCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGc             <  1:176760/145‑1 (MQ=255)

                                                                                                                                 ttGAAACAGATACCATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGCa            >  1:882841/1‑149 (MQ=255)

                                                                                                                                        aGATACCATAGTAATCGGAG‑CACGTCCAGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTACTTAAACGTAGGATGTATTCCTTCAAAAGCATtactac     >  1:732454/1‑149 (MQ=255)

                                                                                                                                           tACCATAGTTATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGCATTACTACATg  <  1:21577/149‑1 (MQ=255)

TAAGCTTTGACCACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAG‑CAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGCATTACTACATG  >  CP000730/1105852‑1106139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A