Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F108 I0 R1 889 70.1 2273064 96.2% 2186687 148.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,188,513 G→T 77.6% G1055V (GGT→GTT)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,188,5130GT77.6% 119.4 / 40.1 76G1055V (GGT→GTT) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base G (5/12);  new base T (32/26);  total (37/39)
Fisher's exact test for biased strand distribution p-value = 9.69e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.38e-01

ATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGG  >  NC_000913/4188369‑4188656
                                                                                                                                                |                                                                                                                                               
aTGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGCCCGgtctg                                                                                                                                             >  2:717699/1‑149 (MQ=255)
 tGGCGAACAGGTTGCTGGCGGCGAAACCGTTTCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATTATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGttctgt                                                                                                                                            <  2:227416/149‑1 (MQ=255)
 tGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACTTGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGttctgt                                                                                                                                            <  1:1094342/149‑1 (MQ=255)
 tGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATAACGCGTCAGACCGACGAACTGACCGttctgt                                                                                                                                            <  2:607032/149‑1 (MQ=255)
    cGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCtt                                                                                                                                         <  1:324750/149‑1 (MQ=255)
     gAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTc                                                                                                                                        <  1:84442/149‑1 (MQ=255)
     gAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTAAGACCGACGAACTGACCGTTCTGTCTTc                                                                                                                                        >  2:697178/1‑149 (MQ=255)
       aCAGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGc                                                                                                                                      <  1:230364/149‑1 (MQ=255)
          ggTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCtgg                                                                                                                                   <  1:530772/149‑1 (MQ=255)
          ggTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCtgg                                                                                                                                   <  2:265554/149‑1 (MQ=255)
           cttGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCtggt                                                                                                                                  >  2:589513/2‑149 (MQ=255)
                tGGCGGCGAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTc                                                                                                                             >  2:836812/1‑149 (MQ=255)
                tGGCGGCGAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTc                                                                                                                             <  2:1127525/149‑1 (MQ=255)
                 ggcggcGAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCt                                                                                                                            <  1:1131549/149‑1 (MQ=255)
                   cggcgAAACCGTTGCAATCTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTgg                                                                                                                          <  1:317275/149‑1 (MQ=255)
                      cgAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGGCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGAtt                                                                                                                       <  2:842823/149‑1 (MQ=255)
                      cgAAACCGTTGCAAACTGGGACCCGCACACCATGCCGCTTATCACCGAAGTAAGCGGTTCTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGATCTGGAtt                                                                                                                       >  1:370814/1‑149 (MQ=255)
                       taaaCCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTATGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGATCTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTc                                                                                                                      <  2:401703/148‑1 (MQ=255)
                       gAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTc                                                                                                                      >  2:576705/1‑149 (MQ=255)
                          aCCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGc                                                                                                                   <  2:752431/149‑1 (MQ=255)
                            cGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAg                                                                                                                 >  1:1027048/1‑149 (MQ=255)
                              ttGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTATTCTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTACTGTCTTCGCAGGTGGTTCTGGATTCCGCAGaa                                                                                                               >  2:992696/1‑149 (MQ=255)
                                  aaaCTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGAGATGCTCGACGGCCAGACCATTACGCGTCAGACCGACGATCAGACCGTTCTGTCTTCGCTGGTGGTGCTGGATTGCTCAGATCGTa                                                                                                           >  2:598279/1‑149 (MQ=255)
                                  aaaCTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTa                                                                                                           >  1:754748/1‑149 (MQ=255)
                                   aaCTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTAc                                                                                                          <  1:866803/149‑1 (MQ=255)
                                    agtGGGACCCGCACTCCATGCGGGTTATCACCGAAGTTAGCGGTTTTGTACGCTTTACTGACATGATGGACGGCCAGAACATTACGCGGCAGAACGACTTACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTAcc                                                                                                         <  1:589513/147‑1 (MQ=255)
                                        ggACCAGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGATGGTGGTTCTGGATTCCGCAGAACGTACCGCAg                                                                                                     >  2:503920/1‑149 (MQ=255)
                                         gACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAgg                                                                                                    <  2:145648/149‑1 (MQ=255)
                                          aCCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAggt                                                                                                   >  1:167146/1‑149 (MQ=255)
                                              gCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGGCATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAggtggtt                                                                                               >  2:574738/1‑148 (MQ=255)
                                               cacaCCATGCCGGTTATCACCGAAGTAAGCGGCTATGTACGCTTTACTGACAAGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTaa                                                                                              <  2:53387/149‑1 (MQ=255)
                                                  acCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGa                                                                                           >  2:408177/1‑149 (MQ=255)
                                                  acCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTGTGGATTCCGCAGAACGTTCCGCAGGTGGTAAAGa                                                                                           <  2:482893/149‑1 (MQ=255)
                                                  acCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGa                                                                                           <  1:892277/149‑1 (MQ=255)
                                                     aTGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCt                                                                                        <  1:387577/149‑1 (MQ=255)
                                                        ccGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCg                                                                                     >  2:340173/1‑149 (MQ=255)
                                                            ttATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCg                                                                                 <  1:751661/149‑1 (MQ=255)
                                                             ttaccacGAAATAACCCGGTTTGTACGGATTACCGCAATGCTCCTCCGCCACCCCATTACCCCTCACCCCGACGCAAAGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCgg                                                                                <  2:204181/143‑1 (MQ=255)
                                                                 aCCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACt                                                                            >  1:188337/1‑149 (MQ=255)
                                                                 aCCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTACTGGATTCCGCAGAACGAACCGCAGGTGGTAAAGATCTGCGTCCGGCAcg                                                                            >  1:288840/1‑148 (MQ=255)
                                                                 aCCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACAGACGAACTGACCGTTATGTCTTCGCTGGTGGTTCTGGAATCCGCAGAACGAACCGCAGGTGGTAAAGATCTGCGTCCGGCAca                                                                            >  1:84006/1‑148 (MQ=255)
                                                                  ccGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGACGTCGCTGGTGGTTCTGGCTTCCACACAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACtt                                                                           >  2:344393/1‑148 (MQ=255)
                                                                          aGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGt                                                                   <  2:754748/149‑1 (MQ=255)
                                                                           gCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGtt                                                                  >  1:607331/1‑149 (MQ=255)
                                                                           gCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTAAAGCAGGTGGTAAACATCTGCGTCCGGCACTGAAATTCgat                                                                  >  2:765925/1‑147 (MQ=255)
                                                                           gCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGGGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGtt                                                                  <  1:988473/149‑1 (MQ=255)
                                                                           gCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGtt                                                                  <  1:185070/149‑1 (MQ=255)
                                                                             ggTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGa                                                                <  2:107842/149‑1 (MQ=255)
                                                                                tttGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGc                                                             >  1:147657/1‑149 (MQ=255)
                                                                                    tACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAg                                                         >  1:761597/1‑149 (MQ=255)
                                                                                     aCGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAgg                                                        <  2:841547/149‑1 (MQ=255)
                                                                                     aCGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGATCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAgg                                                        <  2:370814/149‑1 (MQ=255)
                                                                                       gCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAACATCGTTGATGCTCAGGGt                                                      >  1:742344/1‑149 (MQ=255)
                                                                                       gCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGt                                                      <  1:154140/149‑1 (MQ=255)
                                                                                         tttACTGTCATGATCGGCCGCCAGACCATTACGCGGCAGACCGTCCTACTTACCCTTCTGTCTTCGCTGGGGGGTCTGGCTTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTaa                                                    <  2:514830/149‑1 (MQ=255)
                                                                                              tGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacgacg                                               >  1:105426/1‑149 (MQ=255)
                                                                                              tGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacgacg                                               >  2:1048806/1‑149 (MQ=255)
                                                                                                 cATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTc                                            >  1:734780/1‑149 (MQ=255)
                                                                                                 cATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTc                                            <  2:359374/149‑1 (MQ=255)
                                                                                                    gATCGACGGCCAGGCCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGa                                         <  1:405424/149‑1 (MQ=255)
                                                                                                    gATCGACGGCCAGGCCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGa                                         <  1:132295/149‑1 (MQ=255)
                                                                                                         aCGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGGTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCa                                    <  2:483946/149‑1 (MQ=255)
                                                                                                          cGGCCAGAGCATTACGCGGCAGACCGACGAACTGCCCGGTCTGTCTTCGCTGGTGGTTCTGGAATCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAg                                   <  1:788972/149‑1 (MQ=255)
                                                                                                             ccAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGGCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTa                                >  1:209956/1‑149 (MQ=255)
                                                                                                              cAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTAc                               <  2:159374/149‑1 (MQ=255)
                                                                                                              cAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTAc                               <  1:682515/149‑1 (MQ=255)
                                                                                                                 aCCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGa                            >  1:857019/1‑149 (MQ=255)
                                                                                                                   cATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTGTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGTTCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGata                          <  2:248119/149‑1 (MQ=255)
                                                                                                                   cATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGCTAACGACGTTCTGATCCCAGGTACCGata                          >  1:545620/1‑149 (MQ=255)
                                                                                                                      tACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGc                       >  2:869619/1‑149 (MQ=255)
                                                                                                                           gTCAGACCGACGAACTGCCCGAGCTGTCTTCGCTGGGGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAgcg                  <  2:231663/149‑1 (MQ=255)
                                                                                                                           gTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGGCCGGCACGGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGACCCGATATGCCcgcg                  >  1:994019/1‑149 (MQ=255)
                                                                                                                             cAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCa                >  1:354179/1‑149 (MQ=255)
                                                                                                                               gACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGt              >  1:158873/1‑149 (MQ=255)
                                                                                                                               gACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGAAGCCAGGTCCCGATATGCCCGCGCAGt              >  2:580340/1‑149 (MQ=255)
                                                                                                                                aCCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTa             >  1:53400/1‑149 (MQ=255)
                                                                                                                                  cgacgaACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACt           <  1:4559/149‑1 (MQ=255)
                                                                                                                                      gaACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCt       >  1:1102843/1‑149 (MQ=255)
                                                                                                                                         cTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGcc    >  2:375987/1‑149 (MQ=255)
                                                                                                                                           gACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGGCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCgg  <  1:574738/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
ATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGG  >  NC_000913/4188369‑4188656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A