Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F15 I0 R1 22 59.5 1335882 95.9% 1281110 140.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,279,705 G→A 100% A943V (GCA→GTA)  ebh_3 ← Extracellular matrix‑binding protein ebh

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,279,7050GA100.0% 173.8 / ‑6.5 67A943V (GCA→GTA) ebh_3Extracellular matrix‑binding protein ebh
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (33/32);  minor base C (0/1);  total (33/34)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGA‑TACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGC  >  USA300TCH1516_ALE/2279569‑2279838
                                                                                                                                         |                                                                                                                                      
tgtgCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACtg                                                                                                                                     <  2:450492/140‑1 (MQ=255)
  tgCGGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACtgtt                                                                                                                                   <  2:483002/140‑1 (MQ=255)
     cGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACTgttgtt                                                                                                                                >  1:376748/1‑140 (MQ=255)
         attaGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCTTTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCAc                                                                                                                            <  1:599356/140‑1 (MQ=255)
         attaGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGTATCTGCATTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCAc                                                                                                                            <  1:465144/140‑1 (MQ=255)
         attaGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCAc                                                                                                                            <  2:661985/140‑1 (MQ=255)
             gTTGATTCTACTTCTGGTTTACTAGTTACACCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTCGAATTGTTATTTGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGt                                                                                                                        <  1:498106/140‑1 (MQ=255)
              ttGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGATTGTTTTTAGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGtt                                                                                                                       >  1:422013/1‑140 (MQ=255)
              ttGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATCCATCTACACTAGAATTGTTATTAGCTTGCGATTTATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGtt                                                                                                                       >  1:587521/1‑140 (MQ=255)
                   tCTAGTTCTGGTTTACTAGTTACATCGTTATCCATTTTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGTTGCCGc                                                                                                                  <  2:393594/140‑1 (MQ=255)
                     tactacTGGTTTGCTAGTTACATCGCTATCCATTGTCGGACTGTTTCTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCAACATCACTTGC‑TGATGTTACTGTTGTTTGACCTGTTGCCGCAt                                                                                                                >  1:470086/1‑140 (MQ=255)
                     tACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAAAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGTTGCCGCAt                                                                                                                >  1:365239/1‑140 (MQ=255)
                       cTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATAATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGTTGCCGCATCa                                                                                                              >  1:128431/1‑140 (MQ=255)
                           tGGTTTACTAGTTACATTGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACTCTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACGGTTGTTTCACCTGTTGCCGCATCACtat                                                                                                          <  1:127192/140‑1 (MQ=255)
                           tGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATTCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGTTGCCGCTTCACtat                                                                                                          >  1:243800/1‑140 (MQ=255)
                           tGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGTTGCCGCATCACtat                                                                                                          >  2:155123/1‑140 (MQ=255)
                           tGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGTTGCCGCATAACtat                                                                                                          >  1:243953/1‑140 (MQ=255)
                            ggTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTAAACTAGCATTGTTATTAGCTGGCGGATTAGCATTGGCATCATCAGTTGC‑TGATGTTACTGTTGTTTGACCTGTTGCCGCATCACtatt                                                                                                         >  1:461028/1‑140 (MQ=255)
                                tgcgaGTTAGATCGTTATCCATTTTCGTGCTTGTTGTTTTATCATCTACTCTTGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTAGTAttt                                                                                                     <  2:339299/136‑1 (MQ=255)
                                 aCTAGTTACATCGTTATCCGTTGTCGGACTGTTTGTTGATGCATGTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTg                                                                                                    >  1:364991/1‑140 (MQ=255)
                                 aCTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACAAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTg                                                                                                    >  1:231796/1‑140 (MQ=255)
                                           tCGTTATCCATTGTCGGACTGTTTGTTGATACATATACAATAGAATTGTTATTAGATTGAGGTTTATCTTTTGCATCATAAGTTCA‑TGATGGGACGGTTTTTTCACCTGTGGCCGCATCACTATTATTTGGTGTTGTCgg                                                                                          >  2:157253/1‑140 (MQ=255)
                                             gTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTTTCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGgag                                                                                        <  1:64996/140‑1 (MQ=255)
                                             gTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGgag                                                                                        <  1:612229/140‑1 (MQ=255)
                                                  ccATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCg                                                                                   >  1:254945/1‑140 (MQ=255)
                                                  ccATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCg                                                                                   >  2:574407/1‑140 (MQ=255)
                                                     ttGTCGGACTGTTTGTTGCTGCATCTACACTTGAATTGTTATTAGCTTGCGGTTTTTCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCt                                                                                <  2:509548/140‑1 (MQ=255)
                                                       gTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGTTGCCGCGTCGCTATTATTTGGTGTTGTCGGAGAAGCGTCTGc                                                                              >  2:15877/1‑140 (MQ=255)
                                                       gTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGTTGCCGCATCACGATTATTTGGTGTTGTCGGAGAAGCGTCTGc                                                                              >  1:256342/1‑140 (MQ=255)
                                                          ggACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTCTTATTTGGTGTTGTCGGAGAAGCTTCGGCttt                                                                           >  2:175413/1‑140 (MQ=255)
                                                          ggACTGTTTGTTGATGCAAATACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTACCCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAACGACTgacct                                                                           >  2:289381/1‑136 (MQ=255)
                                                           gACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTg                                                                          <  1:535978/140‑1 (MQ=255)
                                                           gACGGTTTGTTGTTGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTg                                                                          <  2:372609/140‑1 (MQ=255)
                                                           gACCGTTTGTTGAAGCATCTACACTAACATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATAATTTGGAGTTGTCGGAGAAGCGTCTGCTTTg                                                                          <  1:209178/140‑1 (MQ=255)
                                                            aCTTTTTGTTGATTCATCTATACTAGTATTTTTTTTAGTTTGCTGTTTATCTTTTGCATCTTCAGTTGC‑TGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGc                                                                         <  1:124762/140‑1 (MQ=255)
                                                             cTGTTTGTTGATGCATCTTCACTAGAATTGTTATTAGCTTGCGGTTTATCCTTTGCATAATCAGTTAC‑TGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGACTGCTTTGcc                                                                        >  1:249960/1‑140 (MQ=255)
                                                               gttttttGATGCATCTACACTAGAATTGTTATTAGCTTGCTGTTTGTCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTGTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCAt                                                                      <  1:299529/140‑1 (MQ=255)
                                                                tttgttGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGAGGAAGCGTCTGCTTTGCCAtt                                                                     >  2:415938/1‑140 (MQ=255)
                                                                  tgttgATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACTATTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAg                                                                   >  1:230063/1‑140 (MQ=255)
                                                                   tttgATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGc                                                                  <  2:227076/139‑1 (MQ=255)
                                                                   gttgATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGc                                                                  <  2:376748/140‑1 (MQ=255)
                                                                         gCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTgtcgt                                                            >  2:414364/1‑140 (MQ=255)
                                                                         gCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTgtcgt                                                            >  2:612339/1‑140 (MQ=255)
                                                                         gCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCACTAGCTgtcgt                                                            >  2:271909/1‑140 (MQ=255)
                                                                             cTACACTAGAATTGTTATTAGCTTGCGGTTTATCATATGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGATACGGCATCACCATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCa                                                        >  1:484569/1‑140 (MQ=255)
                                                                                 acTTGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGA‑Ta                                                   <  1:379468/140‑1 (MQ=255)
                                                                                 acTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGTTGCCGCATAACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCAGTCGTCTCAGA‑Ta                                                   <  1:639140/140‑1 (MQ=255)
                                                                                  cTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGA‑TAc                                                  <  1:388850/140‑1 (MQ=255)
                                                                                       aTTGTTATTAGCTTGCGGTTTATCTTTTGCATTATTAGTTGC‑TGTTGTTAATGTTGTTTCAGATGTTGCCGCATCACTATTATTTGGTGTTGTGGGGGAAGGGTCTGCTTTGCCATTAGCTGTGGTTTCTGA‑TAGGttgg                                             >  1:12932/1‑138 (MQ=255)
                                                                                            tattaGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGA‑TACGTTAGGTTGt                                        >  2:293910/1‑140 (MQ=255)
                                                                                              ttaGCTTACGGATTATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTTACCTGTTGCCGCATCTCTATTATTTGGTGTTTTCGGATAAGCGCATGCTTTGCCATTAGCTGTCGTTTCAGA‑TACGTTAGTTTGttc                                      >  2:244836/1‑138 (MQ=255)
                                                                                                tggttGCGGGTTATCATTTGCATCATCAGTTAC‑TGATGTCAGTTTTTGTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTTGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGA‑TACGTTAGGTTGTCCAg                                    <  2:138610/137‑1 (MQ=255)
                                                                                                       ggTTTATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGA‑TACGTTAGGTTGTCCAGTATTTTc                             >  1:130655/1‑140 (MQ=255)
                                                                                                          ttATCATTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGTTGCCGCATAACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGA‑TACGTTAGGTTGTCCAGTATTTTCTgg                          >  1:34575/1‑140 (MQ=255)
                                                                                                           tATCATTTGCATCATCAGCTGCATGCTG‑GACTGTTGTTTCACCTGTTGCCGCATCACAATTATTTGGAGTAGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGA‑TACGTTAGGTTGTCCAGTATATTCTGgt                         >  1:160035/1‑140 (MQ=255)
                                                                                                               aTTTGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGTTGCAGCATTACTATTATTTGGTGTTGTCGGAGCAGAGCCGGCTGAGGGTTTAGTTGTCGT‑AAAGAGCACGTTAGGTTGTCCAGTATTTTCGGGTGTTg                     <  2:470086/140‑1 (MQ=255)
                                                                                                                tttGCATCATCAGTTGC‑TGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGA‑TACGTTAGGTTGTCCAGTATTTTCTGGTGTTGc                    <  1:42297/140‑1 (MQ=255)
                                                                                                                   gCATCCTCAGTTGC‑TGATGTTATTGTTGTTTCACTGGTTGCCTCCTCCCGATTATTTGGTTTTGTCGGAGAAGCTCCTGCTTTGCCATTAGCTGTCGTCTCAGA‑TACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCAtt                 <  2:202784/140‑1 (MQ=255)
                                                                                                                    catcctCTGGTGC‑TGATGTTTCTGTGGTTTCCCCTGTTGCCGCATCACTGTTATTTGGTGTTGTCGGAGTAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGA‑TACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTa                <  2:587521/140‑1 (MQ=255)
                                                                                                                        atcaGTTGC‑TGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGA‑TACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCAt            >  1:288551/1‑140 (MQ=255)
                                                                                                                         tcaGTTGC‑TTATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTTCCATTAGCTGTCGTCTCAGA‑TACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCAtt           <  1:171411/140‑1 (MQ=255)
                                                                                                                           aGTTGC‑TGATGTTACTGTTGTTTCAGCTGTGGTCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGTTTTGCCATTAGCTGTCGTCTCAGA‑GACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTg         <  1:635154/140‑1 (MQ=255)
                                                                                                                              tGC‑TGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGA‑TACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAAt      <  2:652206/140‑1 (MQ=255)
                                                                                                                               gC‑TTATTTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGTCATTAGCTGTCGTCTCAGA‑TACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAAtt     <  1:257967/140‑1 (MQ=255)
                                                                                                                               gC‑TTATGTTACTGTTGTTTCACCTTTTGCCGCAGCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGA‑TACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAAtt     <  1:392008/140‑1 (MQ=255)
                                                                                                                                   gctGTTACGGTTGTTTCACCTGTTGCCGCATCCCTATTTTTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGA‑TACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAACTTGc  <  1:452938/138‑1 (MQ=255)
                                                                                                                                   gATGTTCCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGA‑TACGCTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGc  <  2:602648/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                      
TGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGC‑TGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGA‑TACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGC  >  USA300TCH1516_ALE/2279569‑2279838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A