Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F58 I0 R1 46 56.2 1222162 96.0% 1173275 141.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,279,705 G→A 100% A943V (GCA→GTA)  ebh_3 ← Extracellular matrix‑binding protein ebh

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,279,7050GA99.8% 221.7 / ‑7.1 76A943V (GCA→GTA) ebh_3Extracellular matrix‑binding protein ebh
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (26/47);  total (27/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGT  >  USA300TCH1516_ALE/2279567‑2279841
                                                                                                                                           |                                                                                                                                        
gTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTgat                                                                                                                                        >  1:267466/1‑139 (MQ=255)
 ttctgCCGTTATTAGTTGATTCTACTTCTGGTGTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTATAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACtg                                                                                                                                       <  2:344531/138‑1 (MQ=255)
 ttGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTGATATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACtg                                                                                                                                       >  2:556346/1‑141 (MQ=255)
   gtgCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACtgtt                                                                                                                                     >  2:568800/1‑141 (MQ=255)
     gCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACtgttgt                                                                                                                                   <  1:14139/141‑1 (MQ=255)
        gTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCA‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTc                                                                                                                                <  2:391563/141‑1 (MQ=255)
         ttattaGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCGATTGTCGGACTGTTTGCTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGGTGTTACTGTTGTTTCa                                                                                                                               >  2:37206/1‑141 (MQ=255)
              aGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACGGTTGTTTCACCTGt                                                                                                                          <  1:338460/141‑1 (MQ=255)
               gTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGtt                                                                                                                         >  1:586297/1‑141 (MQ=255)
               gTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGtt                                                                                                                         <  2:226749/141‑1 (MQ=255)
               gTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGtt                                                                                                                         <  1:188909/141‑1 (MQ=255)
               gTTCATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGtt                                                                                                                         <  2:340709/141‑1 (MQ=255)
                ttGATTCTACTTCTGGTTTTCTAGTTACACCTTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTTTTATTAGCT‑TGCGGTTTATCATTCGCATCACCAGTTGCTGATGTTACTGTTGTTTCACCTGTTg                                                                                                                        <  2:304298/141‑1 (MQ=255)
                 tGATTCTACTTCCGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGc                                                                                                                       <  1:39041/141‑1 (MQ=255)
                    ttCTACTTCTTGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGc                                                                                                                    <  2:129819/141‑1 (MQ=255)
                        aCTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATcc                                                                                                                >  2:148535/1‑140 (MQ=255)
                         cTTCTGGTTTACTAGTTACATTGTTACCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAAATGTTACTAGCT‑TGCGGCTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCTCCTGTTGCCGCATCAc                                                                                                               <  1:446249/141‑1 (MQ=255)
                           tCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACtt                                                                                                             >  1:223786/1‑140 (MQ=255)
                            cTGGTTTACTAGTTACTGCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACtat                                                                                                            <  2:30934/141‑1 (MQ=255)
                                tttATTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTattatt                                                                                                        <  2:565872/141‑1 (MQ=255)
                                 ttACTTGTTACATCGTTATCCGTTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTAttt                                                                                                       <  2:222843/141‑1 (MQ=255)
                                    ttAGTTACATCGCTGTCCATTTGCGGACTGTTTGTTGATGCATCTACCCTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGgt                                                                                                    <  2:550369/140‑1 (MQ=255)
                                    cTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGgt                                                                                                    <  1:218043/141‑1 (MQ=255)
                                    cTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGgt                                                                                                    <  1:544367/141‑1 (MQ=255)
                                      tgTTACATTGTTTTCTCTTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGgtgt                                                                                                  <  2:47916/140‑1 (MQ=255)
                                       gTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTCTTATTTGGtgtt                                                                                                 >  1:486442/1‑141 (MQ=255)
                                        gtACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGtgttg                                                                                                <  1:584822/140‑1 (MQ=255)
                                              cGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTACCTCTGC‑TATGATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGgag                                                                                          <  1:443872/141‑1 (MQ=255)
                                               gTTATTCTTTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGgaga                                                                                         <  2:1959/141‑1 (MQ=255)
                                               gTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTGTCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGgaga                                                                                         <  1:411471/141‑1 (MQ=255)
                                                 tATCCATTGTCGGACTGTTTGATGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATAATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTAAGCTGTTGCCGCCTCACTATCTTTTGGTGGTGTCGGAGAAg                                                                                       >  2:420055/1‑141 (MQ=255)
                                                  ttCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGGATGGTTATTAGCT‑TGCGGTTTATCATGTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGc                                                                                      <  2:163382/140‑1 (MQ=255)
                                                  aTCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGGGAAGc                                                                                      >  1:82765/1‑141 (MQ=255)
                                                  aTCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAgg                                                                                      >  1:179200/1‑140 (MQ=255)
                                                     cATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTGTCGTTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCAGCACGATTATTTGGTGTTGTCGGAGAAGCggt                                                                                   >  1:90190/1‑139 (MQ=255)
                                                            cgACTGTNTGTTGGTGCATCTACACTAGAATTGTTATNAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGNATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTg                                                                            <  2:434743/140‑1 (MQ=255)
                                                               cTGCTTGTAGATGCATCTACACTACAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCa                                                                         <  1:6029/141‑1 (MQ=255)
                                                                tgtttgttGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCAt                                                                        >  1:346321/1‑141 (MQ=255)
                                                                    tgttgATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGc                                                                    <  2:424945/141‑1 (MQ=255)
                                                                    tgttgATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGc                                                                    <  1:190280/141‑1 (MQ=255)
                                                                     gttgATGCATCTTCAATAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCt                                                                   <  2:288471/141‑1 (MQ=255)
                                                                      ttgATGCATCTACACTACAATAGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTTTTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTg                                                                  >  1:146833/1‑141 (MQ=255)
                                                                       tgATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTgt                                                                 >  2:604433/1‑141 (MQ=255)
                                                                           gCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTCGTCGGCGATAGGTCTGCTTGGCCATTAGCtgttgtt                                                             >  2:398093/1‑140 (MQ=255)
                                                                            cATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGtct                                                            >  2:74226/1‑141 (MQ=255)
                                                                             aacTACTCTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGtctc                                                           <  1:266623/139‑1 (MQ=255)
                                                                              tctaaccTAGAATTGTTATTAGCT‑TGCGGTTTATCTTTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCa                                                          <  2:377646/135‑1 (MQ=255)
                                                                              tcggctcTATAATTGTTATTAGCT‑TGCGGTTTATCCTTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCa                                                          <  2:301137/135‑1 (MQ=255)
                                                                               cTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAg                                                         <  1:556346/141‑1 (MQ=255)
                                                                                tACACTAGAATTGTTATTACCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGGCGCATCACTATTATTTGGCGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGa                                                        <  1:37206/141‑1 (MQ=255)
                                                                                     tAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGt                                                   <  1:41511/141‑1 (MQ=255)
                                                                                     tAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTACTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGt                                                   <  1:408123/141‑1 (MQ=255)
                                                                                         aTTGTTATTAGCT‑TGCGGTTTATCATTTGCATTGTGAGTTGGTGTTGTTACTTTTATTTCACCTATTTCTTCATCACTATTATTTCCTGTTTTCGGATAATCGTCTTCTTTGCCATTAGCTGTCGTCTCAGATACGTTAgg                                               <  2:447199/141‑1 (MQ=255)
                                                                                            gTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTg                                            >  2:480727/1‑141 (MQ=255)
                                                                                            gTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTAGCCATTAGCTGTCGTCTCAGATACGTTAGGTTg                                            >  2:340731/1‑141 (MQ=255)
                                                                                                ttaGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTCGGTTGTccc                                        >  2:423837/1‑140 (MQ=255)
                                                                                                ttaGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCa                                        <  1:88743/141‑1 (MQ=255)
                                                                                                  tagttggCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAg                                       <  2:609221/135‑1 (MQ=255)
                                                                                                     t‑tGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTAtt                                   <  2:380734/141‑1 (MQ=255)
                                                                                                       tGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTAttt                                  >  1:571716/1‑141 (MQ=255)
                                                                                                         tgttttATCATTTTCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTc                                <  2:115467/138‑1 (MQ=255)
                                                                                                         cGGTTTATCATTTGCATCCTAAGGGCCCGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTc                                >  2:588085/1‑141 (MQ=255)
                                                                                                                     tGCATCATCAGTTGCTGATGTTACTGTAGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGTTGTTGCAtt                    >  2:130702/1‑141 (MQ=255)
                                                                                                                      gCATCATCCGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTa                   <  2:64866/141‑1 (MQ=255)
                                                                                                                      gCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGTTGTTGCATTa                   >  1:135030/1‑141 (MQ=255)
                                                                                                                         tcatcaGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCa                >  2:133105/1‑141 (MQ=255)
                                                                                                                           atcaGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCAtt              >  2:318084/1‑141 (MQ=255)
                                                                                                                             caGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGGGTTGCATTAGCATTTg            >  1:494070/1‑141 (MQ=255)
                                                                                                                              aGTGGCTGATGTTACTGTTGTTTCCCCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGa           <  1:275588/141‑1 (MQ=255)
                                                                                                                                 tGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCGTTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAAtt        <  2:298385/141‑1 (MQ=255)
                                                                                                                                 tGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAAtt        <  1:148535/141‑1 (MQ=255)
                                                                                                                                   cTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCGCGCGTGGCATTAGCATTTGAATTTg      <  1:604433/141‑1 (MQ=255)
                                                                                                                                    tGATGTCTCTGTTTTTTCCCCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGc     <  1:123022/141‑1 (MQ=255)
                                                                                                                                      ttGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTg   <  2:363245/140‑1 (MQ=255)
                                                                                                                                      tggTTGCTGTTTCTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTg   <  2:363350/139‑1 (MQ=255)
                                                                                                                                       tGTTACTGTTGTTTCACCTGTTGGCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGt  <  2:75494/141‑1 (MQ=255)
                                                                                                                                       tGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGt  >  1:539593/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                        
GTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCT‑TGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGT  >  USA300TCH1516_ALE/2279567‑2279841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A