Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F121 I0 R1	45	69.8	1582072	96.2%	1521953	141.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,279,705	G→A	100%	A943V (GCA→GTA)	ebh_3 ←	Extracellular matrix‑binding protein ebh

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,279,705	0	G	A	100.0%	240.7 / NA	77	A943V (GCA→GTA)	ebh_3	Extracellular matrix‑binding protein ebh
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (39/38); total (39/38)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGT  >  USA300TCH1516_ALE/2279568‑2279841

ttGAGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACtg                                                                                                                                        <  2:3825/141‑1 (MQ=255)

   tgCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACtgttg                                                                                                                                     >  1:740323/1‑141 (MQ=255)

   tgCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACtgttg                                                                                                                                     >  1:282638/1‑141 (MQ=255)

      cGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGttt                                                                                                                                  >  1:132206/1‑141 (MQ=255)

         tattaGTTGATTCTACTTCTGGTTTACTTGTTTCATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑Ac                                                                                                                              <  1:124675/141‑1 (MQ=255)

          attaGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑Acc                                                                                                                             <  1:135533/141‑1 (MQ=255)

          attaGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑Acc                                                                                                                             >  1:278958/1‑141 (MQ=255)

          attaGTTGATTCTAATTCTGGTTTACTAGTTACATCGTTATCCAGTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑Acc                                                                                                                             >  1:431446/1‑141 (MQ=255)

            tcgTTGATTCTTCTTCTGGTTTACTAGTTTCATCGTTATCCTTTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTg                                                                                                                           <  2:694134/139‑1 (MQ=255)

            taGTTGATTCTTCTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCCTCAGTGTCTGATGTTACTGTTGTTTC‑ACCTg                                                                                                                           <  2:169365/141‑1 (MQ=255)

              gTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGtt                                                                                                                         >  2:61766/1‑141 (MQ=255)

               ttGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTg                                                                                                                        <  1:341723/141‑1 (MQ=255)

                   ttCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGc                                                                                                                    <  1:684386/141‑1 (MQ=255)

                    tCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGAACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCAGCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGct                                                                                                                   >  1:14316/1‑140 (MQ=255)

                     tttcTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCAt                                                                                                                  <  2:261763/138‑1 (MQ=255)

                       aCTTCTGGTTTACTAGTTACGTCGGTATCCATTGTCGGACTGTGTGTTGATGCATCCTCACTTGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCa                                                                                                                <  1:64643/141‑1 (MQ=255)

                       aCTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATcc                                                                                                                >  2:576963/1‑140 (MQ=255)

                       aCTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCa                                                                                                                <  1:329217/141‑1 (MQ=255)

                       aCTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCa                                                                                                                >  2:790170/1‑141 (MQ=255)

                        cTTCTGGTTTACTAGTCCCAACACGATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAACTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGGTGTTACTGTTGTTTC‑ACCTGTTGCCGCATcgc                                                                                                               >  1:383669/1‑139 (MQ=255)

                          tCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCTGCATCACta                                                                                                             >  1:648532/1‑141 (MQ=255)

                          tCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACta                                                                                                             >  2:17845/1‑141 (MQ=255)

                          tCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTCC‑ACCTGTTGCCGCATCACta                                                                                                             >  2:350971/1‑141 (MQ=255)

                          tCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATAAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCAGCATGACta                                                                                                             >  2:636023/1‑141 (MQ=255)

                           tgggTTTACTAGTTACATTGTTTTCCATTGTCGGACTGTTTGTTCATGCATCTACCCTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCGGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACtat                                                                                                            <  2:377984/139‑1 (MQ=255)

                               tttACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTattatt                                                                                                        <  1:247754/141‑1 (MQ=255)

                                 tACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTg                                                                                                      >  1:341140/1‑141 (MQ=255)

                                 tACCAGTTACCGCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTg                                                                                                      <  1:730268/141‑1 (MQ=255)

                                    tAGTTACATCTTTATCCATTGTCGGACTGTTTGTTGCTGCATCTACACTAGTATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTTATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGgtg                                                                                                   <  1:353825/141‑1 (MQ=255)

                                       ttACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTgttgttt                                                                                                >  1:335796/1‑140 (MQ=255)

                                         acttCGTTTTCCATTGTCGGACTGTTTGTTGAGGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCAGTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTc                                                                                              <  2:120826/138‑1 (MQ=255)

                                         aCATCGTTATCCATGTTCGGATTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTc                                                                                              <  1:83173/141‑1 (MQ=255)

                                            tCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATTATCTGTTGCTGATGTTACGTTTCTTTCGAGGTG‑TGCCGCATCACTATTATTTGGTGTTGTCGga                                                                                           <  2:556025/141‑1 (MQ=255)

                                              gTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGgaga                                                                                         >  1:249172/1‑141 (MQ=255)

                                                    cATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGGGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTc                                                                                   <  2:359343/141‑1 (MQ=255)

                                                     aTTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTAGTCGGAGAAGCGTCt                                                                                  >  1:92940/1‑141 (MQ=255)

                                                      ttGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCCTCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTg                                                                                 >  1:303156/1‑141 (MQ=255)

                                                      ttGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTg                                                                                 >  1:51089/1‑141 (MQ=255)

                                                          cGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCttt                                                                             <  1:398293/141‑1 (MQ=255)

                                                           ggccTGTTTGTTGGTGCATCTACACTAGAATTGTTATTAGATTGGGGTTTATCATGTGCATCGTCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCGCTATTATTTGGTGTTGTGGGCGCACCGTCTGCTTTg                                                                            >  2:360839/4‑141 (MQ=255)

                                                              cTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTTTGGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCa                                                                         <  2:281749/141‑1 (MQ=255)

                                                              cTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCACCAGTTGCTGATGCTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCa                                                                         <  2:281755/141‑1 (MQ=255)

                                                               tgtttgttGATGCATCTACACTAGGATTGTTTCTAGCTTGCGGTTCATCATTTGCATCACCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTTTTTGAAATTGTCGTAGAAGCGTCTGCTTTGCCAt                                                                        <  1:189256/141‑1 (MQ=255)

                                                               tgtttgttGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCAt                                                                        >  2:678026/1‑141 (MQ=255)

                                                                  ttgttgATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTTGGAGAAGCGTCTGCTTTGCCATTAg                                                                     >  1:493792/1‑141 (MQ=255)

                                                                   tgttgATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGc                                                                    <  2:655982/141‑1 (MQ=255)

                                                                     ttgATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑CCCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAGGCGTCTGCTTTGCCATTAGCTg                                                                  >  1:135986/1‑141 (MQ=255)

                                                                        aTGCATCTACACTAGAATTGATATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTgtcg                                                               >  1:475503/1‑141 (MQ=255)

                                                                          gCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTgtcgtc                                                             <  2:266722/141‑1 (MQ=255)

                                                                               tACACTAGAATTGTTATTGGCTTGCGGTTTATCCTTTGTATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGa                                                        <  2:208401/141‑1 (MQ=255)

                                                                               tACACTAATATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACGATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGa                                                        <  1:370262/141‑1 (MQ=255)

                                                                                        aTTGTTATTAGCTTCCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAgg                                               <  2:648532/141‑1 (MQ=255)

                                                                                             tattaGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTc                                          >  2:75162/1‑141 (MQ=255)

                                                                                                  gCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCTTTAGCTGTCGTGTCAGATACGTTAGGTTGTCCGGtt                                     >  2:493215/1‑140 (MQ=255)

                                                                                                  gCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGGTAGGTTGTCCAGtt                                     >  1:2750/1‑140 (MQ=255)

                                                                                                       cGGTTTATCTTTTGCTTCATCAGTTGCTGTTGTTACTGTTGTTTC‑TCCTTTTGCCGCATCACTATTATTTGGTGTTGTCGGAGATGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTc                                <  2:417296/141‑1 (MQ=255)

                                                                                                        ggTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCACATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCt                               >  1:525038/1‑141 (MQ=255)

                                                                                                         gTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTg                              <  2:132206/141‑1 (MQ=255)

                                                                                                          tttGTCATTTGCTTCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTgg                             <  1:288158/141‑1 (MQ=255)

                                                                                                           ttATCATTTGCATCATCCGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGgt                            <  1:177933/141‑1 (MQ=255)

                                                                                                           ttATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTATGgt                            >  1:686045/1‑141 (MQ=255)

                                                                                                             aTCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGgtgt                          <  2:393181/141‑1 (MQ=255)

                                                                                                                 tttGCATCATTCGTTGCCGATGTTACTGTTGTTTC‑CCCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCCCAAACTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTCGCa                      <  1:615241/141‑1 (MQ=255)

                                                                                                                      atcatcAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGc                 >  1:765203/1‑141 (MQ=255)

                                                                                                                          tcaGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCAttt             <  1:430862/141‑1 (MQ=255)

                                                                                                                           caGTTGCTGATGTTACTGTTGTTTC‑ACGTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGCTACGTTAGGTTGTACAGTATTTTCTGGTGTTGCATTAGCGTTTg            >  2:459753/1‑141 (MQ=255)

                                                                                                                             gTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGTGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTTGGTTGTCAAGTATTTTCTGGTGTTGCATTAGCATTTGaa          >  1:19852/1‑141 (MQ=255)

                                                                                                                             gTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGaa          <  1:790170/141‑1 (MQ=255)

                                                                                                                             gTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGaa          >  1:640190/1‑141 (MQ=255)

                                                                                                                             gTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTATGGTGTTGCATTAGCATTTGaa          >  2:516185/1‑141 (MQ=255)

                                                                                                                              ttGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCGTTAGCATTTGAAt         >  2:291573/1‑141 (MQ=255)

                                                                                                                              ttGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAAt         <  2:303156/141‑1 (MQ=255)

                                                                                                                                  tGATGTTACTGTTGTTTC‑GCCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGc     <  1:395133/141‑1 (MQ=255)

                                                                                                                                  tGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCCGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGc     >  2:415613/1‑141 (MQ=255)

                                                                                                                                  tGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGc     <  1:622414/141‑1 (MQ=255)

                                                                                                                                  tGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGc     >  2:234743/1‑141 (MQ=255)

                                                                                                                                     tGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGAATTAGCATTTGAATTTGCTGt  >  2:593761/1‑141 (MQ=255)

TTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGT  >  USA300TCH1516_ALE/2279568‑2279841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A