Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F178 I1 R1	28	89.4	2015306	95.8%	1930663	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,279,705	G→A	A943V (GCA→GTA)	ebh_3 ←	Extracellular matrix‑binding protein ebh

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,279,705	0	G	A	99.1%	281.9 / NA	108	A943V (GCA→GTA)	ebh_3	Extracellular matrix‑binding protein ebh
Reads supporting (aligned to +/- strand): ref base G (0/1); major base A (51/54); minor base C (1/0); total (52/56)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

TTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCT  >  USA300TCH1516_ALE/2279568‑2279839

gtgggtcGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACt                                                                                                                                      <  1:249345/134‑1 (MQ=255)

 tgtgCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCACTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTGTCATTTGCATCATCAGTTGCTGATGTTACtg                                                                                                                                     >  1:672308/1‑140 (MQ=255)

 tgtgCCGTTATTAATTGATTCTACTTCTGGTTTACTAGTTACAACGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATAAGCTTGCGGTTTACCATTTGCATCAACAGTTGCAGACGTTACtg                                                                                                                                     >  2:421142/1‑140 (MQ=255)

     ccGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACttgtgt                                                                                                                                 >  1:509338/1‑135 (MQ=255)

      cGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATTCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTgttgtt                                                                                                                                <  1:80839/140‑1 (MQ=255)

      cGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTgttgtt                                                                                                                                <  2:41639/140‑1 (MQ=255)

        ttattaGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTc                                                                                                                              <  1:971895/140‑1 (MQ=255)

           ttaGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCAcc                                                                                                                           >  2:173692/1‑140 (MQ=255)

           ttaGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCAcc                                                                                                                           <  1:175673/140‑1 (MQ=255)

               ttGATTCTACTTCTTGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATTCATCTACACTGGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGtt                                                                                                                       <  2:69344/140‑1 (MQ=255)

                       aCTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATc                                                                                                               >  1:259266/1‑140 (MQ=255)

                       aCTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATc                                                                                                               >  1:509596/1‑140 (MQ=255)

                       aCTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATc                                                                                                               >  2:875326/1‑140 (MQ=255)

                       aCTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGGTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATc                                                                                                               >  1:918588/1‑140 (MQ=255)

                        cTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCa                                                                                                              <  2:542248/140‑1 (MQ=255)

                        cTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCa                                                                                                              >  1:923351/1‑140 (MQ=255)

                         ttCTGGTTTACTAGTTACATCGTTTTCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCAc                                                                                                             <  1:183763/140‑1 (MQ=255)

                          tCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCCCt                                                                                                            >  2:923615/1‑140 (MQ=255)

                          tCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACt                                                                                                            >  1:108834/1‑140 (MQ=255)

                          tCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACt                                                                                                            <  1:806849/140‑1 (MQ=255)

                          tCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCAGCACt                                                                                                            >  1:112758/1‑140 (MQ=255)

                           cTGGTTTACTTGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACta                                                                                                           <  1:25620/140‑1 (MQ=255)

                           cTGGTTGACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACta                                                                                                           <  1:164079/140‑1 (MQ=255)

                            tGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACtat                                                                                                          >  1:351609/1‑140 (MQ=255)

                              ttttACTATTTACATCGTTATTCATCGTCGGACGGTTTGTTTATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCTGCGGGTGCCGCATCACtatta                                                                                                        <  2:216317/139‑1 (MQ=255)

                              gtctACTAGTTACATCGTTATCCATTGTCGGGCTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACtatta                                                                                                        <  2:672417/137‑1 (MQ=255)

                              ggttACTAGTGACATCGTTTTCCATTGTCGGACTGGTTGTGGATGCATCTACACTACAATTGTTATTAGCTGGCGGTTTTTCAGTTGCATCATCAGTTGCTGATGTTGCTGTTGGGTCGCCTTTCGCCGCATCACtgtga                                                                                                        <  2:284708/138‑5 (MQ=255)

                               tttACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCCCtatttt                                                                                                       >  2:78065/1‑138 (MQ=255)

                               tttACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACtattat                                                                                                       >  2:565456/1‑140 (MQ=255)

                               tttACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACtattat                                                                                                       >  2:898914/1‑140 (MQ=255)

                                 tACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCGCTATTAttt                                                                                                     >  2:364837/1‑140 (MQ=255)

                                  aCTAGTTGAATGGGTATCCATTGCCGGACTGTGTGGTGATGCAGCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTg                                                                                                    <  1:225027/140‑1 (MQ=255)

                                   cTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGCTGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTATTGTTGTTTCCCCTGTTGCCGCATCACTATTATTTgg                                                                                                   <  2:839363/140‑1 (MQ=255)

                                    tAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGgt                                                                                                  >  2:871638/1‑140 (MQ=255)

                                         aCATCGTTATCCATTGTCGGACTGTTTGTTGATGCTTCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGtgttgt                                                                                             <  2:667493/140‑1 (MQ=255)

                                               ttATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGgtga                                                                                       >  1:81234/1‑140 (MQ=255)

                                               ttATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGgaga                                                                                       >  2:331869/1‑140 (MQ=255)

                                               ttATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTCTCGAATCACTATTATTTGGTGTTGTCGgaga                                                                                       >  1:311568/1‑140 (MQ=255)

                                               ttATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTAATCATTTGAAACATCAGTTGCTGATGTTACTGTTGTTTAACGTGTTTCCGAATCACTACTTTTTGGTGTTGTCGtagg                                                                                       >  2:447953/1‑139 (MQ=255)

                                                tctCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCGTTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGGATCACTATTATTTGGTGTTGTCTGAGaa                                                                                      >  2:925236/3‑140 (MQ=255)

                                                tctCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGCAGaa                                                                                      >  2:366206/3‑140 (MQ=255)

                                                tATCCATTGTCGGACTGTTTGTTGATGCATCTTCACTTGTTTTTTTTTTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCGGTTCTCGCATCACTCTTATTTGTTTTTATCggtaga                                                                                      >  1:582712/1‑136 (MQ=255)

                                                  tCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGGGAAGc                                                                                    >  1:461201/1‑140 (MQ=255)

                                                    cactGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGt                                                                                  <  1:444335/137‑1 (MQ=255)

                                                      ttGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCt                                                                                >  1:627603/1‑140 (MQ=255)

                                                        gTCGGACTGTTTGTTGATGCATCTCCACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCCCTATTATTTGGTGTTGTCGGAGAAGCGTCTGc                                                                              >  2:114638/1‑140 (MQ=255)

                                                          cGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATGATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCCTCACTATTCTTTGGTGTGGTCGGAGAAGCGCCTGCtc                                                                            >  1:825795/1‑139 (MQ=255)

                                                          cGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGGAGCGGCTGCtt                                                                            >  1:421674/1‑140 (MQ=255)

                                                           ggACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCttt                                                                           >  1:173451/1‑140 (MQ=255)

                                                             aCTGTTTGGTGATGCATCTAACCTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGc                                                                         <  1:107972/140‑1 (MQ=255)

                                                              cTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGcc                                                                        <  1:918049/140‑1 (MQ=255)

                                                              cTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGcc                                                                        <  1:249387/140‑1 (MQ=255)

                                                                gtttgttGATGCATCTACACTAGATTTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCCCCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCAt                                                                      <  2:994876/140‑1 (MQ=255)

                                                                  ttgttgATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTa                                                                    >  2:984499/1‑140 (MQ=255)

                                                                  ttgttgATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGGTGTCGGAGAAGCGTCTGCTTTGCCAttg                                                                    >  2:272777/1‑139 (MQ=255)

                                                                   tgttgATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCTTTAg                                                                   >  2:452819/1‑140 (MQ=255)

                                                                            ttCTACTCTAGAATTTTTATTAGCTTTCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGCGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGtct                                                          <  2:654019/139‑1 (MQ=255)

                                                                             tCTACACTAGAATTGTTATTAGCTTGCGTTTTATAATTTGCATCATCAGTTGCTGAGGTTACTCTTGTTTCACCTGTTGCCGCATCACTTTTATTTGGTGTTTTGTGAGCAGCGTCTGCTTTGCGTTTTGCTGTCGtctc                                                         >  2:469953/1‑140 (MQ=255)

                                                                                acacTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCCTTGCCATTAGCTGTCGTCTCAGa                                                      >  1:896238/1‑140 (MQ=255)

                                                                                 cacTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGAt                                                     <  1:567067/140‑1 (MQ=255)

                                                                                  acTAGAATTGGTATGTGCTTTCTGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATa                                                    <  2:116872/140‑1 (MQ=255)

                                                                                     agcaTTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGt                                                 <  1:447953/137‑1 (MQ=255)

                                                                                        aTTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGTAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGTTACGTTAg                                              >  2:53668/1‑140 (MQ=255)

                                                                                         ttGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAgg                                             <  1:871638/140‑1 (MQ=255)

                                                                                               ttaGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTCCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATCCTTTAGGTTGTcc                                       >  2:849604/1‑140 (MQ=255)

                                                                                                 aGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCGGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGTTACGTTAGGTTGTcctg                                     >  1:170630/1‑138 (MQ=255)

                                                                                                 aGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAg                                     >  1:93101/1‑140 (MQ=255)

                                                                                                 aGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTATTTCACATGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGGTAGGTTGTCCAg                                     >  1:46624/1‑140 (MQ=255)

                                                                                                  gCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGGCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGt                                    <  1:309427/140‑1 (MQ=255)

                                                                                                          tttATCTGTTCCATCATCTGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTg                            <  2:150877/140‑1 (MQ=255)

                                                                                                          tttATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTg                            <  2:351609/140‑1 (MQ=255)

                                                                                                          tttATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTg                            >  1:148483/1‑140 (MQ=255)

                                                                                                           ttATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCGCTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTCGGTTGGCCAGTATTTTCTgg                           >  1:128116/1‑140 (MQ=255)

                                                                                                           ttATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTTTgg                           >  1:170954/1‑140 (MQ=255)

                                                                                                            tATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTTTCCAGTACTTGCTGgt                          >  1:79817/1‑140 (MQ=255)

                                                                                                              tcctttGTATCACCAGTTGCTGTTTTTACTTTTTTTTCACCGCTTGCCACATCACTATTATTTGGGGTTGTCGGATAAGCGTCTGCTTTGTCATTAGCTGTCGTTGCATATACGTTAGTTTGTCCAGTATTTTCTGgtgt                        <  2:144108/137‑1 (MQ=255)

                                                                                                              tCATTTGCATCATCCGTTGCTGATGTTACTGTTGTTTCGCCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCAAGTATTTTCTGgtgt                        <  1:504549/140‑1 (MQ=255)

                                                                                                              tCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGTTGTTGTCGGAGAAGCGTCTGCTTTGCCATCAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGgtgt                        <  1:173692/140‑1 (MQ=255)

                                                                                                               cgtttGCATCATCAGTTGCTTATGTTACGTTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGtt                       <  2:460343/138‑1 (MQ=255)

                                                                                                               cATTTGGCTCATTAGTTGTTGATGTTACGTTTTTTTCCCCTGTTGCTGCATCACTATTATTTGCTGTTGTCTGAGACGCCTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGtt                       <  2:17987/140‑1 (MQ=255)

                                                                                                                aTTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTg                      >  2:887622/1‑140 (MQ=255)

                                                                                                                 tttGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGTATCACTATTTTTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGc                     <  2:458510/140‑1 (MQ=255)

                                                                                                                 tctGCAGCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGc                     <  2:99317/138‑1 (MQ=255)

                                                                                                                           caGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCAttt           <  1:408165/140‑1 (MQ=255)

                                                                                                                           caGTTGCTGATGTTACTGGTGTTTCCCCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTTAGATACTTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCAttt           <  1:969202/140‑1 (MQ=255)

                                                                                                                            aGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTCTTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTg          >  2:939093/1‑140 (MQ=255)

                                                                                                                            aGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTCTTTGGTGTTGTCGGAAATGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTg          >  2:87316/1‑140 (MQ=255)

                                                                                                                            aGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTg          <  1:955809/140‑1 (MQ=255)

                                                                                                                              ttGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGaa        <  2:766845/140‑1 (MQ=255)

                                                                                                                               tGCTTATGTGACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGTAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAAt       <  2:147265/140‑1 (MQ=255)

                                                                                                                               tGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAAt       >  2:397468/1‑140 (MQ=255)

                                                                                                                               tGCTGATGTTACTGTTGTTGCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAAt       <  1:363226/140‑1 (MQ=255)

                                                                                                                               tGCTGATGTTACTGTGGTGTCACCTGTTGCCGGATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAAt       <  1:343156/140‑1 (MQ=255)

                                                                                                                               tGCAGATGTTACCGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAAt       <  1:482945/140‑1 (MQ=255)

                                                                                                                               gtcTGATGTTATTGTTGTTTCGCCTGTTGCCGCACTACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGGCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAAt       <  2:868034/138‑1 (MQ=255)

                                                                                                                                  tGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTATGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTg    <  1:675909/140‑1 (MQ=255)

                                                                                                                                  tGATGTGACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTg    <  1:119031/140‑1 (MQ=255)

                                                                                                                                   taTGTTTCTGTTGTTCCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTTTTGCATTAGCATTTGAATTTGc   <  2:774789/139‑1 (MQ=255)

                                                                                                                                   gATGTTACTGTTGTTTCCCCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTTGTCTTGCATTAGCATTTGAATTTGc   <  2:259266/140‑1 (MQ=255)

                                                                                                                                    aTGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCt  <  2:81234/140‑1 (MQ=255)

TTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCT  >  USA300TCH1516_ALE/2279568‑2279839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A