Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F28 I3 R2
|
122 |
64.1 |
3058059 |
97.6% |
2984665 |
63.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
782,109 |
T→C |
Q167R (CAG→CGG) |
hlyE ← |
hemolysin E |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 782,109 | 0 | T | C | 100.0%
| 179.5
/ NA
| 52 | Q167R (CAG→CGG) | hlyE | hemolysin E |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (25/27); total (25/27) |
TGGACCGGCGACGACACCGGCTGCGGCACCGGCATATGCTTCCTTCCTGATTTTATCTACCTGTGACTGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGTTTCCCGG > minE/782042‑782175
|
tGGACCGGCGACGACACCGGCTGCGGCACCGGCATATGCTTCCTTCCTGATTTTATCTACCTGTGACCgg > 1:1394068/1‑70 (MQ=255)
tGGACCGGCGACGACACCGGCTGCGGCACCGGCATATGCTTCCTTCCTGATTTTATCTACCTGTGACCgg > 1:2575497/1‑70 (MQ=255)
tGGACCGGCGACGACACCGGCTGCGGCACCGGCATATGCTTCCTTCCTGATTTTATCTACCTGTGACCGGa < 1:2027761/71‑1 (MQ=255)
gCGACGACACCGGCTGCGGCACCGGCATATGCTTCCTTCCTGATTTTATCTACCTGTGACCGGaaa < 1:2747051/66‑1 (MQ=255)
gCGACGACACCGGCTGCGGCACCGGCATATGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAgct < 1:2129490/71‑1 (MQ=255)
gacACCGGCTGCGGCACCGGCATATGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCttt < 1:2629204/71‑1 (MQ=255)
acacCGGCTGCGGCACCGGCATATGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCtttt < 1:2252259/71‑1 (MQ=255)
ccGGCTGCGGCACCGGCATATGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTc > 1:1201772/1‑71 (MQ=255)
ccGGCTGCGGCACCGGCATATGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTc < 1:329942/71‑1 (MQ=255)
cGGCTGCGGCACCGGCATATGCTTCCTTCCTGATTTTATCTACCTGTGACCGGaaa < 1:1679789/56‑1 (MQ=255)
gCTGCGGCACCGGCATATGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCtttttt > 1:56152/1‑67 (MQ=255)
cTGCGGCACCGGCATATGCTTCCTTCCTGATTTTATCTACCTGTGACCTGAAATAGCTGCTTTTTTCTGa < 1:2614921/70‑1 (MQ=255)
cTGCGGCACCGGCATATGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGaa < 1:2700055/71‑1 (MQ=255)
tGCGGCACCGGCATATGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCt > 1:2926008/1‑67 (MQ=255)
cGGCACCGGCATATGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAgctgc < 1:2649087/57‑1 (MQ=255)
ggCACCGGCATATGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAAt > 1:661080/1‑71 (MQ=255)
gCACCGGCATATGCTTCCTTCCTGATTTTATCTACCTGTGACCGGaaa < 1:2058327/48‑1 (MQ=255)
cACCGGCATATGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCa > 1:2371674/1‑71 (MQ=255)
aCCGGCATATGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGaaa < 1:1533907/65‑1 (MQ=255)
ccGGCATATGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAAt < 1:919909/46‑1 (MQ=255)
ccGGCATATGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGaaaaa < 1:2740209/66‑1 (MQ=255)
ccGGCATATGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCAtt > 1:2241636/1‑71 (MQ=255)
cGGCATATGCTTCCTTCCTGATTTTATCTACCTGTGACCGGaa > 1:294010/1‑43 (MQ=255)
gCATATGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGt > 1:948736/1‑71 (MQ=255)
cATATGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGtt < 1:2501971/71‑1 (MQ=255)
tatGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTaa < 1:1753112/71‑1 (MQ=255)
tGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATa > 1:1585921/1‑39 (MQ=255)
tGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACt < 1:1516048/71‑1 (MQ=255)
gCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTg > 1:1301394/1‑71 (MQ=255)
cttccttcCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCAt < 1:1396689/60‑1 (MQ=255)
cttccttcCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTgg < 1:676083/71‑1 (MQ=255)
cttccttcCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTg < 1:2191966/70‑1 (MQ=255)
tccttcctGATTTTATCTACCTGTGTCCGGAAATAgctg > 1:389586/1‑39 (MQ=38)
ccttcctGATTTTATCTACCTGTGACCGGAAATAgctg > 1:959058/1‑38 (MQ=255)
ccttcctGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGc < 1:3031502/69‑1 (MQ=255)
cttcctGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAAc > 1:331834/1‑64 (MQ=255)
ttcctGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTa < 1:2345859/61‑1 (MQ=255)
ttcctGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTa < 1:2961109/61‑1 (MQ=255)
ttcctGATTTTATCTACCTGTCACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTa < 1:1970854/61‑1 (MQ=255)
ctGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTa < 1:142543/70‑1 (MQ=255)
aTTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGc < 1:192117/71‑1 (MQ=255)
aTTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGc > 1:2514297/1‑71 (MQ=255)
tttATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGc > 1:2641499/1‑69 (MQ=255)
tttATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGc > 1:1808911/1‑69 (MQ=255)
cTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGt > 1:259105/1‑71 (MQ=255)
cTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGt > 1:1489399/1‑71 (MQ=255)
aCCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCa > 1:2745309/1‑39 (MQ=255)
cTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGTTTcc > 1:1731778/1‑71 (MQ=255)
tgtgACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATc > 1:2695840/1‑53 (MQ=255)
tgACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGTTTCCCgg < 1:1042780/71‑1 (MQ=255)
gACCGGAAATAGCTGCTTTTTTCTGAAAAATCAtt > 1:2231194/1‑35 (MQ=255)
gACCGGAAATAGCTGCTTTTTTCTGAAAAATCAtt > 1:752477/1‑35 (MQ=255)
|
TGGACCGGCGACGACACCGGCTGCGGCACCGGCATATGCTTCCTTCCTGATTTTATCTACCTGTGACTGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGTTTCCCGG > minE/782042‑782175
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
