Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F21 I0 R1 57 0.0 661283 90.4% 597799 68.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 782,109 T→C 100% Q167R (CAG→CGG)  hlyE ← hemolysin E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE782,1090TC100.0% 289.0 / NA 76Q167R (CAG→CGG) hlyEhemolysin E
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/48);  total (28/48)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGCTTCCTTCCTGATTTTATCTACCTGTGACTGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGTTT  >  minE/782078‑782170
                               |                                                             
tGCTTCCTTCCTGATTTTATCTACCTGTGACCGGTAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACt                        >  1:156285/1‑71 (MQ=255)
tGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATc                                   >  1:389244/1‑60 (MQ=255)
tGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTaa                          >  1:267644/1‑69 (MQ=255)
tGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACt                        >  1:562853/1‑71 (MQ=255)
tGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACt                        >  1:294572/1‑71 (MQ=255)
tGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACt                        >  1:3451/1‑71 (MQ=255)
tGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACt                        >  1:350734/1‑71 (MQ=255)
tGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACt                        >  1:385133/1‑71 (MQ=255)
tGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACt                        >  1:426561/1‑71 (MQ=255)
tGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACt                        >  1:496813/1‑71 (MQ=255)
tGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACt                        >  1:530777/1‑71 (MQ=255)
tGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACt                        >  1:531611/1‑71 (MQ=255)
tGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACt                        >  1:417534/1‑71 (MQ=255)
tGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACt                        >  1:569501/1‑71 (MQ=255)
tGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACt                        >  1:608394/1‑71 (MQ=255)
tGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACt                        >  1:609144/1‑71 (MQ=255)
tGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACt                        >  1:637122/1‑71 (MQ=255)
tGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACt                        >  1:82325/1‑71 (MQ=255)
tGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACt                        >  1:82518/1‑71 (MQ=255)
tGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACt                        >  1:91447/1‑71 (MQ=255)
tGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACt                        >  1:96786/1‑71 (MQ=255)
tGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACt                        >  1:98607/1‑71 (MQ=255)
tGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACt                        >  1:10953/1‑71 (MQ=255)
tGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACt                        >  1:134471/1‑71 (MQ=255)
tGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACt                        >  1:273952/1‑71 (MQ=255)
tGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACt                        >  1:238237/1‑71 (MQ=255)
tGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACt                        >  1:176848/1‑71 (MQ=255)
tGCTTCCTTCCTGATTTTATCTACCTGTGACCGGAAATAGCTGCTTTTTTATGAAAAATCATTGGTTAACt                        >  1:311023/1‑71 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:60938/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:191307/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:153719/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:157436/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:585382/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:567813/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:182738/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:549223/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:535020/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:534276/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:197281/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:612707/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:616028/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:621349/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:630439/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:633155/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:149319/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:651797/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:142412/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:142132/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:83991/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:139663/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:529170/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:276168/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:2493/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:355140/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:367082/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:371250/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:375148/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:382579/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:387343/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:22896/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:415800/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:10362/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:220834/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:435103/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:45202/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:455402/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:483554/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:484991/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:493448/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:197890/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:500195/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGctt  <  1:280775/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGctt  <  1:281523/70‑1 (MQ=255)
                       ccTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACCGGCTATCTAACGCCAGCAGttt  <  1:88240/70‑1 (MQ=255)
                        cTGTGACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGttt  <  1:360927/69‑1 (MQ=255)
                          gtgACCGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTATCGCCAGCAGttt  <  1:634368/67‑1 (MQ=255)
                               |                                                             
TGCTTCCTTCCTGATTTTATCTACCTGTGACTGGAAATAGCTGCTTTTTTCTGAAAAATCATTGGTTAACTGGCTATCTAACGCCAGCAGTTT  >  minE/782078‑782170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A