Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I3 R1	283	58.3	1819865	98.8%	1798026	150.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	944,703	G→A	G224D (GGT→GAT)	dmsC →	dimethyl sulfoxide reductase, anaerobic, subunit C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	944,703	0	G	A	100.0%	202.5 / NA	53	G224D (GGT→GAT)	dmsC	dimethyl sulfoxide reductase, anaerobic, subunit C
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (26/27); total (26/27)

GGCTGGGCGATGCGTCTGCTGCCAGCAATTTCTGTACTGGCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGGTGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTGGCAGGGGAATTAATCGGT  >  NC_000913/944564‑944842

ggCTGGGCGATGCGTCTGCTGCCAGCAATTTCTGTACTGGCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGt                                                                                                                                  >  1:478903/1‑151 (MQ=255)

 gCTGGGCGATGCGTCTGCTGCCAGCAATTTCTGTACTGGCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTc                                                                                                                                 >  1:578894/1‑151 (MQ=255)

       cGATGCGTCTGCTGCCAGCAATTTCTGTACTGGCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCg                                                                                                                           <  1:451918/151‑1 (MQ=255)

       cGATGCGTCTGCTGCCAGCAATTTCTGTACTGGCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCg                                                                                                                           >  1:805964/1‑151 (MQ=255)

                        gCAATTTCTGTACTGGCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCg                                                                                                          >  2:110624/1‑151 (MQ=255)

                         cAATTTCTGTACTGGCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGt                                                                                                         >  1:802017/1‑151 (MQ=255)

                           aTTTCTGTACTGGCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTg                                                                                                       >  1:291846/1‑151 (MQ=255)

                                 gTACTGGCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCtg                                                                                                  <  2:732145/150‑1 (MQ=255)

                                      ggCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTtg                                                                                            <  1:375856/151‑1 (MQ=255)

                                      ggCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTtg                                                                                            <  2:558041/151‑1 (MQ=255)

                                        cACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTtgtg                                                                                          >  1:885347/1‑151 (MQ=255)

                                            ggTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGAtt                                                                                      <  1:543880/151‑1 (MQ=255)

                                            ggTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGAt                                                                                       <  1:668127/150‑1 (MQ=255)

                                              tAGTGAGAGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGc                                                                                    >  2:816862/1‑151 (MQ=255)

                                               agtgagtgGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGc                                                                                    <  1:335034/150‑1 (MQ=255)

                                                           ggtgTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTaaaa                                                                       <  1:196370/151‑1 (MQ=255)

                                                           ggtgTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTaaa                                                                        <  1:533854/150‑1 (MQ=255)

                                                              gtCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAgg                                                                     <  1:81600/150‑1 (MQ=255)

                                                                cAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGtt                                                                   <  1:509749/150‑1 (MQ=255)

                                                                cAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGtt                                                                   <  2:779079/150‑1 (MQ=255)

                                                                     aTGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGc                                                             >  2:591080/1‑151 (MQ=255)

                                                                       gCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCt                                                           >  2:3782/1‑151 (MQ=255)

                                                                         aGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGc                                                         >  2:81208/1‑151 (MQ=255)

                                                                            gcgcAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGt                                                      <  2:257154/151‑1 (MQ=255)

                                                                              gcAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTc                                                    >  2:886033/1‑151 (MQ=255)

                                                                                  agCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCt                                                >  1:327108/1‑151 (MQ=255)

                                                                                    cTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTaaaa                                                                       <  1:827495/126‑1 (MQ=255)

                                                                                    cTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTaaaa                                                                       >  2:827428/1‑126 (MQ=255)

                                                                                     tGGCGACTATTCATAG‑TCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACtt                                            >  1:391935/1‑151 (MQ=255)

                                                                                      ggCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACtt                                            >  2:840943/1‑151 (MQ=255)

                                                                                      ggCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACt                                             <  2:529293/150‑1 (MQ=255)

                                                                                       gCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACttt                                           <  1:133413/151‑1 (MQ=255)

                                                                                         gACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCt                                         >  2:811167/1‑151 (MQ=255)

                                                                                          aCTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGTATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTg                                        <  2:879393/151‑1 (MQ=255)

                                                                                          aCTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTCCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTg                                        >  2:123735/1‑151 (MQ=255)

                                                                                             aTTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGt                                       <  2:78191/149‑1 (MQ=255)

                                                                                                   aGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTAtcattca                               <  2:259897/151‑1 (MQ=255)

                                                                                                   aGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTAtcattca                               <  1:409403/151‑1 (MQ=255)

                                                                                                            cagcagGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCtt                       >  2:147091/1‑150 (MQ=255)

                                                                                                            cagcagGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTc                      >  1:719146/1‑151 (MQ=255)

                                                                                                             agcagGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCt                     >  2:670368/1‑151 (MQ=255)

                                                                                                              gcagGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTg                    <  1:569284/151‑1 (MQ=255)

                                                                                                                   ccGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTGGCAgg               <  2:622159/151‑1 (MQ=255)

                                                                                                                       aGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCGGTTGCCCTGTGCTTGGGGATTGCACCACGGCTAAAAGGTTATCAGCCTGGGGTTCCGCTCCTTTTTGTATCATTCATTCTGCTTCTGCCGGGGGa            >  1:306017/1‑150 (MQ=255)

                                                                                                                       aGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTGGCAGGGGa            >  2:598998/1‑150 (MQ=255)

                                                                                                                            tGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTg                                        >  1:421127/1‑117 (MQ=255)

                                                                                                                            tGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTg                                        <  2:421082/117‑1 (MQ=255)

                                                                                                                             ggTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTGGCAGGGGAATTAAt      <  1:378193/150‑1 (MQ=255)

                                                                                                                             ggTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTGGCAGGGGAATTAATc     <  2:803917/151‑1 (MQ=255)

                                                                                                                             ggTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTGGCAGGGGAATTAATc     >  1:659254/1‑151 (MQ=255)

                                                                                                                               tGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTGGCAGGGTAATTAATCg    <  1:189535/150‑1 (MQ=255)

                                                                                                                                gCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTGGCAGGGGAATTAATCGGt  <  1:90449/151‑1 (MQ=255)

                                                                                                                                gCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTGGCAGGGGAATTAATCGGt  >  1:848809/1‑151 (MQ=255)

GGCTGGGCGATGCGTCTGCTGCCAGCAATTTCTGTACTGGCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGGTGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTGGCAGGGGAATTAATCGGT  >  NC_000913/944564‑944842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A