Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	257	49.9	1554294	99.0%	1538751	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	944,703	G→A	G224D (GGT→GAT)	dmsC →	dimethyl sulfoxide reductase, anaerobic, subunit C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	944,703	0	G	A	100.0%	151.4 / NA	40	G224D (GGT→GAT)	dmsC	dimethyl sulfoxide reductase, anaerobic, subunit C
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/20); total (20/20)

TGCGTCTGCTGCCAGCAATTTCTGTACTGGCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGGTGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTGGCAGGGGAATTAATCGGTCGCGGCGT  >  NC_000913/944574‑944850

tGCGTCTGCTGCCAGCAATTTCTGTACTGGCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGAt                                                                                                                                >  2:93721/1‑151 (MQ=255)

  cGTCTGCTGCCAGCAATTTCTGTACTGGCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCg                                                                                                                              >  2:712386/1‑151 (MQ=255)

            cAGCAATTTCTGTACTGGCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGc                                                                                                                    >  1:185850/1‑151 (MQ=255)

                aaTTTCTGTACTGGCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGt                                                                                                                 >  2:480749/1‑150 (MQ=255)

                       gTACTGGCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCtgt                                                                                                         >  1:659502/1‑151 (MQ=255)

                           tGGCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCtt                                                                                                     >  2:190288/1‑151 (MQ=255)

                            ggCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTtg                                                                                                    >  2:499788/1‑151 (MQ=255)

                                  ggTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGa                                                                                                <  1:138764/149‑1 (MQ=255)

                                  ggTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGAtt                                                                                              <  1:583829/151‑1 (MQ=255)

                                              cGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCt                                                                                   >  2:51918/1‑150 (MQ=255)

                                               gtggtgTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTa                                                                                  >  1:585884/1‑150 (MQ=255)

                                                 ggtgTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTaaaa                                                                               <  2:335023/151‑1 (MQ=255)

                                                  gtgtCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAg                                                                              <  1:23349/151‑1 (MQ=255)

                                                    gtCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGt                                                                            <  2:685354/151‑1 (MQ=255)

                                                    gtCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGt                                                                            <  1:263572/151‑1 (MQ=255)

                                                           aTGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGc                                                                     <  2:185991/151‑1 (MQ=255)

                                                               aGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGc                                                                 >  2:324659/1‑151 (MQ=255)

                                                               aGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGc                                                                 <  2:228074/151‑1 (MQ=255)

                                                                     cAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTcc                                                           >  1:440131/1‑151 (MQ=255)

                                                                     cAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTcc                                                           <  1:689045/151‑1 (MQ=255)

                                                                     cAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTcc                                                           <  2:177919/151‑1 (MQ=255)

                                                                         gCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCt                                                        >  2:530507/1‑150 (MQ=255)

                                                                           tGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACt                                                     >  2:338105/1‑151 (MQ=255)

                                                                               gACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCt                                                 <  2:75835/151‑1 (MQ=255)

                                                                                         aGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTAtcattc                                        >  2:130790/1‑150 (MQ=255)

                                                                                               gtgCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCattcattc                                    <  1:171966/148‑1 (MQ=255)

                                                                                                 gcagcaGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCtt                               <  1:195997/151‑1 (MQ=255)

                                                                                                  cagcagGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTc                              >  1:758848/1‑151 (MQ=255)

                                                                                                  cagcagGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTc                              <  1:425170/151‑1 (MQ=255)

                                                                                                   agcagGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCt                             <  1:226610/151‑1 (MQ=255)

                                                                                                          cGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTGGCAggg                      >  2:636415/1‑151 (MQ=255)

                                                                                                               cgcTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTGGCAGGGGAAtt                 <  2:26705/151‑1 (MQ=255)

                                                                                                                   ggTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTGGCAGGGGAATTAATc             <  2:114494/151‑1 (MQ=255)

                                                                                                                    gTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTGGCAGGGGAATTAATCg            <  1:211685/151‑1 (MQ=255)

                                                                                                                     tGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTGGCAGGGGAATTAATCgg           >  2:515909/1‑151 (MQ=255)

                                                                                                                      gCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTGGCAGGGGAATTAATCGGt          <  2:28044/151‑1 (MQ=255)

                                                                                                                       ccGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTGGCAGGGGAATTAATCGGTc         <  1:50631/151‑1 (MQ=255)

                                                                                                                         ggACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTGGCAGGGGAATTAATCGGTcgc       >  1:3478/1‑151 (MQ=255)

                                                                                                                         ggACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTGGCAGGGGAATTAATCGGTcgc       >  1:218274/1‑151 (MQ=255)

                                                                                                                             tATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTGGCAGGGGAATTAATCGGTCgcggcg   >  1:706598/1‑151 (MQ=255)

                                                                                                                              atgatgCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTGGCAGGGGAATTAATCGGTCGCGGCGt  >  2:710219/1‑151 (MQ=255)

TGCGTCTGCTGCCAGCAATTTCTGTACTGGCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGGTGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTGGCAGGGGAATTAATCGGTCGCGGCGT  >  NC_000913/944574‑944850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A