Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I3 R1 283 58.3 1819865 98.8% 1798026 150.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,042,663 A→G R516R (CGT→CGC)  etk ← tyrosine‑protein kinase, role in O‑antigen capsule formation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,042,6630AG100.0% 226.2 / NA 64R516R (CGT→CGC) etktyrosine‑protein kinase, role in O‑antigen capsule formation
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/36);  total (28/36)

TAAATAACACTTTTTGATCGGACTGGGCGATCACCGCTGCCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCACGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGC  >  NC_000913/1042515‑1042809
                                                                                                                                                    |                                                                                                                                                  
taaataaCACTTTTTGATCGGACTGGGCGATCACCGCTGCCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGcgcgcg                                                                                                                                                  >  2:691213/1‑151 (MQ=255)
   ataaCACTTTTTGATCGGACTGGGCGATCACCGCTGCCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTAc                                                                                                                                               <  2:80754/151‑1 (MQ=255)
       cacTTTTTGATCGGACTGGGCGATCACCGCTGCCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCt                                                                                                                                           >  2:884881/1‑151 (MQ=255)
        acTTTTTGATCGGACTGGGCGATCACCGCTGCCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCtt                                                                                                                                          >  1:389029/1‑151 (MQ=255)
               gATCGGACTGGGCGATCACCGCTGCCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCAcagc                                                                                                                                   >  1:81567/1‑151 (MQ=255)
                 tCGGACTGGGCGATCACCGCTGCCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCAcagc                                                                                                                                   >  2:282528/1‑149 (MQ=255)
                   ggACTGGGCGATCACCGCTGCCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGa                                                                                                                                >  2:318293/1‑150 (MQ=255)
                     aCTGGGCGATCACCGCTGCCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATc                                                                                                                             <  1:469881/151‑1 (MQ=255)
                           cGATCACCGCTGCCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCgg                                                                                                                       >  1:653273/1‑151 (MQ=255)
                            gATCACCGCTGCCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCggg                                                                                                                      >  2:726169/1‑151 (MQ=255)
                            gATCACCGCTGCCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCggg                                                                                                                      >  1:304430/1‑151 (MQ=255)
                                 ccGCTGCCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTAt                                                                                                                  <  1:857009/150‑1 (MQ=255)
                                  cGCTGCCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATcc                                                                                                                <  2:797534/151‑1 (MQ=255)
                                        cAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGcc                                                                                                          >  1:895960/1‑151 (MQ=255)
                                          gagTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCa                                                                                                         <  2:93386/150‑1 (MQ=255)
                                          gagTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAg                                                                                                        <  1:336955/151‑1 (MQ=255)
                                          gagTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAg                                                                                                        <  2:599015/151‑1 (MQ=255)
                                            gTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAgg                                                                                                       >  2:533568/1‑150 (MQ=255)
                                            gTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGa                                                                                                      <  1:689341/151‑1 (MQ=255)
                                              tGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGaa                                                                                                     <  2:714102/150‑1 (MQ=255)
                                                aaCTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAggg                                                                                                  >  2:782526/1‑151 (MQ=255)
                                                    gACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGAATGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAAGCCGCCGGGTTATCCACCGCCAGGAAGGGGaga                                                                                              <  2:410095/151‑3 (MQ=255)
                                                        aaCGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATAtttt                                                                                          >  1:441450/1‑151 (MQ=255)
                                                          cGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATAttttt                                                                                         >  2:391322/1‑150 (MQ=255)
                                                          cGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTa                                                                                        <  1:18776/151‑1 (MQ=255)
                                                           gTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAg                                                                                       <  2:80699/151‑1 (MQ=255)
                                                              ttACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTa                                                                                     <  2:402713/150‑1 (MQ=255)
                                                                      gTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGgc                                                                              <  2:465525/149‑1 (MQ=255)
                                                                      gTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGgc                                                                              <  2:183705/149‑1 (MQ=255)
                                                                        cTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCgctg                                                                          >  2:441526/1‑151 (MQ=255)
                                                                          ggCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCgctgc                                                                         <  1:793470/150‑1 (MQ=255)
                                                                           gCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGctgctg                                                                       <  2:13056/151‑1 (MQ=255)
                                                                            cGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGa                                                                      <  1:745695/151‑1 (MQ=255)
                                                                             gTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGAt                                                                     >  2:322782/1‑151 (MQ=255)
                                                                               cGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTa                                                                   <  2:816039/151‑1 (MQ=255)
                                                                                    cGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGaaa                                                               >  1:646760/1‑150 (MQ=255)
                                                                                    cGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGaaa                                                               >  2:667207/1‑150 (MQ=255)
                                                                                        gATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAaaataa                                                           <  2:698806/150‑1 (MQ=255)
                                                                                         aTCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAaaataaat                                                         >  1:253388/1‑151 (MQ=255)
                                                                                         aTCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAaaataaat                                                         <  2:683821/151‑1 (MQ=255)
                                                                                         aTCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAaaataaat                                                         <  2:669240/151‑1 (MQ=255)
                                                                                           cATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAAttt                                                       >  2:262164/1‑151 (MQ=255)
                                                                                            aTTAGAATGTTATGCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGTAGAATCCGCCGGGTTATCCTCCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAAttt                                                       <  2:228987/150‑1 (MQ=255)
                                                                                              tAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAAttttt                                                     <  2:413285/150‑1 (MQ=255)
                                                                                               aGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCt                                                   >  1:224610/1‑151 (MQ=255)
                                                                                                 aaTGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTa                                                 <  1:832249/151‑1 (MQ=255)
                                                                                                      tATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCa                                             <  1:214966/150‑1 (MQ=255)
                                                                                                      tATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCa                                             <  1:260959/150‑1 (MQ=255)
                                                                                                       aTTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAg                                            <  1:698226/150‑1 (MQ=255)
                                                                                                            ccGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACgg                                        <  2:826776/149‑1 (MQ=255)
                                                                                                            ccGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACg                                         <  2:645385/148‑1 (MQ=255)
                                                                                                              gTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGc                                    >  2:576326/1‑151 (MQ=255)
                                                                                                                  ccATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGt                                  <  2:734733/149‑1 (MQ=255)
                                                                                                                    atcatAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGa                                           <  1:107896/138‑1 (MQ=255)
                                                                                                                    atcatAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGa                                           >  2:107874/1‑138 (MQ=255)
                                                                                                                       atAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATcc                            >  1:404998/1‑150 (MQ=255)
                                                                                                                       atAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCa                           <  1:867653/151‑1 (MQ=255)
                                                                                                                             aaaTGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACt                     <  1:235993/151‑1 (MQ=255)
                                                                                                                              aaTGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTc                    >  1:445881/1‑151 (MQ=255)
                                                                                                                                tGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCgg                  >  2:183055/1‑151 (MQ=255)
                                                                                                                                   aGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGa                 >  2:45976/1‑149 (MQ=255)
                                                                                                                                   aGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGAc                >  2:360399/1‑150 (MQ=255)
                                                                                                                                          ttCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGa         <  2:206847/150‑1 (MQ=255)
                                                                                                                                               aGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTgg   >  2:265333/1‑151 (MQ=255)
                                                                                                                                               aGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTgg   <  1:183170/151‑1 (MQ=255)
                                                                                                                                                gcgcgcGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGc  <  2:585725/151‑1 (MQ=255)
                                                                                                                                                gcgcgcGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAAAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTgg   <  2:751506/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TAAATAACACTTTTTGATCGGACTGGGCGATCACCGCTGCCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCACGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGC  >  NC_000913/1042515‑1042809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A