Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I2 R1 257 49.9 1554294 99.0% 1538751 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,042,663 A→G R516R (CGT→CGC)  etk ← tyrosine‑protein kinase, role in O‑antigen capsule formation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,042,6630AG100.0% 195.3 / NA 56R516R (CGT→CGC) etktyrosine‑protein kinase, role in O‑antigen capsule formation
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/27);  total (29/27)

TTTTTGATCGGACTGGGCGATCACCGCTGCCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCACGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGAT  >  NC_000913/1042525‑1042803
                                                                                                                                          |                                                                                                                                            
tttttGATCGGACTGGGCGATCACCGCTGCCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTcc                                                                                                                                  >  2:465769/1‑151 (MQ=255)
     gATCGGACTGGGCGATCACCGCTGCCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCAcagc                                                                                                                             <  2:644727/151‑1 (MQ=255)
         ggACTGGGCGATCACCGCTGCCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGaa                                                                                                                         <  2:766269/151‑1 (MQ=255)
                   aTCACCGCTGCCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGt                                                                                                               >  1:1635/1‑151 (MQ=255)
                      aCCGCTGCCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTAt                                                                                                            >  1:75180/1‑151 (MQ=255)
                      aCCGCTGCCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTAt                                                                                                            >  2:727198/1‑151 (MQ=255)
                              cAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGcc                                                                                                    <  1:544504/151‑1 (MQ=255)
                               agagTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCa                                                                                                   <  2:736110/151‑1 (MQ=255)
                                  gTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAgg                                                                                                 <  1:766439/150‑1 (MQ=255)
                                    tGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGaa                                                                                               <  1:1582/150‑1 (MQ=255)
                                    tGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGa                                                                                                >  1:16328/1‑149 (MQ=255)
                                     gAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAg                                                                                              <  1:457292/150‑1 (MQ=255)
                                       aCTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAgggg                                                                                           <  1:338073/151‑1 (MQ=255)
                                          gACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGata                                                                                        <  2:35087/151‑1 (MQ=255)
                                           aCAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGatat                                                                                       <  2:142156/151‑1 (MQ=255)
                                               aCGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATAttttt                                                                                   >  1:28229/1‑151 (MQ=255)
                                                          cTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATgg                                                                         <  1:408151/150‑1 (MQ=255)
                                                          cTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGgc                                                                        >  2:765799/1‑151 (MQ=255)
                                                          cTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGgc                                                                        >  2:570923/1‑151 (MQ=255)
                                                          cTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTAAGATGgc                                                                        >  2:42155/1‑151 (MQ=255)
                                                            gTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGgcgc                                                                      >  2:384066/1‑151 (MQ=255)
                                                                  cGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGctgctg                                                                 >  1:750589/1‑150 (MQ=255)
                                                                    tCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGAtt                                                              >  2:86511/1‑151 (MQ=255)
                                                                     cGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGAtt                                                              <  1:385639/150‑1 (MQ=255)
                                                                        aCCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGa                                                           >  1:85533/1‑150 (MQ=255)
                                                                         ccGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGaaa                                                         <  1:476677/151‑1 (MQ=255)
                                                                         ccGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGaa                                                          >  2:697857/1‑150 (MQ=255)
                                                                         ccGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGaa                                                          >  1:130115/1‑150 (MQ=255)
                                                                                tCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAAtt                                                  >  2:617500/1‑151 (MQ=255)
                                                                                  aTTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAAtttt                                                <  1:224130/151‑1 (MQ=255)
                                                                                       aaTGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTa                                           <  1:517781/151‑1 (MQ=255)
                                                                                         tGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACg                                         >  2:624262/1‑151 (MQ=255)
                                                                                          gTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACg                                         <  1:79775/150‑1 (MQ=255)
                                                                                            tATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCa                                       <  1:202209/150‑1 (MQ=255)
                                                                                              ttCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGAc                                    >  2:229684/1‑151 (MQ=255)
                                                                                                   cGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTg                               <  2:450001/151‑1 (MQ=255)
                                                                                                    gTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGc                              >  2:579108/1‑151 (MQ=255)
                                                                                                     tctcCATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAAtttt                                                >  1:417282/1‑132 (MQ=255)
                                                                                                     tctcCATCAGAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAAtttt                                                <  2:417333/132‑1 (MQ=255)
                                                                                                        ccATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGGTGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCg                             <  1:755352/148‑1 (MQ=255)
                                                                                                        ccATCATAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGttt                          <  1:670001/151‑1 (MQ=255)
                                                                                                          atcatAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTAt                        >  2:309479/1‑151 (MQ=255)
                                                                                                             atAGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCa                     <  2:8024/151‑1 (MQ=255)
                                                                                                               aGCGAAATGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGc                   >  1:560479/1‑151 (MQ=255)
                                                                                                                   aaaTGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACt               <  1:737895/151‑1 (MQ=255)
                                                                                                                    aaTGCAGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTc              >  2:142185/1‑151 (MQ=255)
                                                                                                                         aGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAg                                      >  2:117765/1‑122 (MQ=255)
                                                                                                                         aGACTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAg                                      <  1:117733/122‑1 (MQ=255)
                                                                                                                         aGACTGGATCGAAACGCGCGAACGGCTTCCACCGCAGAATCCGCCGGGTTATCTACCGACAGGAAGGGGCTATTTTTAGAACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGATGGGTTTATCCAGCGACTCGGAc          >  1:125319/1‑150 (MQ=255)
                                                                                                                           aCTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACAtt       >  2:31015/1‑151 (MQ=255)
                                                                                                                            cTGGTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACAtt       <  1:399801/150‑1 (MQ=255)
                                                                                                                              ggTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTgg     <  2:530264/150‑1 (MQ=255)
                                                                                                                              ggTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTgg     <  2:363579/150‑1 (MQ=255)
                                                                                                                               gTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTggg    >  2:316884/1‑150 (MQ=255)
                                                                                                                               gTTCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTggg    >  1:758074/1‑150 (MQ=255)
                                                                                                                                 tCGTAGCGCGCGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGAt  >  2:352413/1‑150 (MQ=255)
                                                                                                                                          |                                                                                                                                            
TTTTTGATCGGACTGGGCGATCACCGCTGCCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCATCATAGCGAAATGCAGACTGGTTCGTAGCGCACGTACGGCTTCCACAGCAGAATCCGCCGGGTTATCCACCGCCAGGAAGGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGAT  >  NC_000913/1042525‑1042803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A