Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F50 I2 R1
|
257 |
49.9 |
1554294 |
99.0% |
1538751 |
150.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,192,714 |
A→G |
C354R (TGC→CGC) |
mnmA ← |
tRNA(Gln,Lys,Glu) U34 2‑thiouridylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,192,714 | 0 | A | G | 100.0%
| 176.2
/ NA
| 49 | C354R (TGC→CGC) | mnmA | tRNA(Gln,Lys,Glu) U34 2‑thiouridylase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (34/15); total (34/15) |
CAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCGGTCTGGCGATAGCGGGTTTTTACCGTGCA > NC_000913/1192569‑1192858
|
cAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACtt > 1:736695/1‑151 (MQ=255)
ggCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTc < 2:63906/150‑1 (MQ=255)
ggCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTc > 1:421255/1‑150 (MQ=255)
gACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTtata < 1:645017/151‑1 (MQ=255)
cAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGAc > 2:736123/1‑151 (MQ=255)
cAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGAc > 1:641130/1‑151 (MQ=255)
aaTACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACgg > 2:507644/1‑151 (MQ=255)
aCCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGc > 1:151308/1‑149 (MQ=255)
aCCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCa < 2:742335/150‑1 (MQ=255)
cGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGAc > 2:705552/1‑151 (MQ=255)
ggCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACt > 2:686796/1‑151 (MQ=255)
cAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGc > 1:576360/1‑151 (MQ=255)
aGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGcc < 2:106010/151‑1 (MQ=255)
ggTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCa < 2:56170/151‑1 (MQ=255)
gATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCAcg < 1:307124/150‑1 (MQ=255)
aTGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCAcggc < 2:404578/151‑1 (MQ=255)
tCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTc > 2:427703/1‑150 (MQ=255)
tCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTc < 1:739218/150‑1 (MQ=255)
tttGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATc > 1:747046/1‑151 (MQ=255)
ttGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCa < 2:600307/148‑1 (MQ=255)
ccACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGaa > 1:92702/1‑150 (MQ=255)
aCGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAAt > 1:264039/1‑151 (MQ=255)
gTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATc > 2:420778/1‑150 (MQ=255)
gTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCa < 2:617872/151‑1 (MQ=255)
ttCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCAc > 1:60578/1‑151 (MQ=255)
gCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCa < 2:510593/149‑1 (MQ=255)
gCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAAt > 1:597891/1‑151 (MQ=255)
cTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCAACCGAGGAGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTAATCGAAAATAACTTCAAt > 1:428842/1‑150 (MQ=255)
ccTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCg < 2:73977/150‑1 (MQ=255)
gTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGatcat > 1:48075/1‑151 (MQ=255)
gTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATAACGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGatcat > 1:521262/1‑151 (MQ=255)
ttttAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGatcatc > 1:327365/1‑151 (MQ=255)
aaGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTcc > 1:129381/1‑151 (MQ=255)
aaaGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAgcg > 2:311148/1‑151 (MQ=255)
aaaGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAgcg > 1:19993/1‑151 (MQ=255)
gATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCt > 1:274255/1‑151 (MQ=255)
ataataATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCtt < 2:268755/151‑1 (MQ=255)
aatCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACgg > 2:176091/1‑151 (MQ=255)
gACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCa > 2:773672/1‑150 (MQ=255)
agcggcagACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGatat > 2:482306/1‑151 (MQ=255)
gACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCGGTCTg > 1:9703/1‑151 (MQ=255)
aCGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCGGTCTgg > 2:586430/1‑151 (MQ=255)
tgctCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGGCCAGCGCCTTGACGGTGCAAGGGATATCGGTCTGTCGAt > 1:569078/1‑151 (MQ=255)
ataataCCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGACGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCGGTCTGGCGATAGCggg < 2:666269/151‑1 (MQ=255)
taataCCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCGGTCTGGCGATAGCggg > 2:146687/1‑150 (MQ=255)
ataCCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCGGTCTGGCGATAGCGGGtt > 1:411876/1‑150 (MQ=255)
gCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCGGTCTGGCGATAGCGGGTTTTTAcc < 2:404722/151‑1 (MQ=255)
cACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCGGTCTGGCGATAGCGGGTTTTTACCg > 1:504953/1‑150 (MQ=255)
gAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCGGTCTGGCGATAGCGGGTTTTTACCGTGCa > 2:381088/1‑150 (MQ=255)
|
CAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCGGTCTGGCGATAGCGGGTTTTTACCGTGCA > NC_000913/1192569‑1192858
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
