Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I2 R1 193 39.9 1341687 91.8% 1231668 149.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,399,151 Δ1 bp coding (1740/1848 nt) mutL → methyl‑directed mismatch repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,399,1510G.100.0% 245.6 / NA 51coding (1740/1848 nt)mutLmethyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (27/24);  total (27/24)

CAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATCAGTAAGGCGAGCCTGCCTAAGGCGATTTTTTTG  >  NC_000913/4399005‑4399296
                                                                                                                                                  |                                                                                                                                                 
caacaaAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGc                                                                                                                                              <  1:216752/151‑1 (MQ=255)
           aCAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAAc                                                                                                                                   <  1:403162/151‑1 (MQ=255)
              aaTCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGt                                                                                                                                >  2:314912/1‑151 (MQ=255)
                          tGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAAc                                                                                                                    >  2:615900/1‑151 (MQ=255)
                           gAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAAc                                                                                                                    <  2:171232/150‑1 (MQ=255)
                            aaCTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACtt                                                                                                                  <  2:191615/151‑1 (MQ=255)
                             aCTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTtg                                                                                                                 >  2:240284/1‑151 (MQ=255)
                              cTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTtgt                                                                                                                >  2:638161/1‑151 (MQ=255)
                              cTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTtgt                                                                                                                >  1:461951/1‑151 (MQ=255)
                               tGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTtgtg                                                                                                               >  1:6980/1‑151 (MQ=255)
                                gATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTtgtg                                                                                                               >  1:634855/1‑150 (MQ=255)
                                 aTAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTtgt                                                                                                                <  2:76972/148‑1 (MQ=255)
                                   aGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGaaa                                                                                                            >  1:657869/1‑150 (MQ=255)
                                         ccTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAAcgccgc                                                                                                     >  2:392297/1‑151 (MQ=255)
                                             gCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCggg                                                                                                 <  2:305764/151‑1 (MQ=255)
                                              cGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGggt                                                                                                <  1:587767/151‑1 (MQ=255)
                                               gAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGggtg                                                                                               >  1:291198/1‑151 (MQ=255)
                                               gAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGggtg                                                                                               <  1:480906/151‑1 (MQ=255)
                                                     gTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGt                                                                                         >  2:475514/1‑151 (MQ=255)
                                                     gTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGt                                                                                         <  1:84145/151‑1 (MQ=255)
                                                      tCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAAAGCCGCCGGGCGGTCTGtt                                                                                        >  1:385298/1‑151 (MQ=255)
                                                        cGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTAc                                                                                      <  2:508123/151‑1 (MQ=255)
                                                             tCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCt                                                                                 >  2:297567/1‑151 (MQ=255)
                                                                aaCCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCt                                                                                 <  1:431917/148‑1 (MQ=255)
                                                                aaCCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGtt                                                                              >  1:424255/1‑151 (MQ=255)
                                                                aaCCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGtt                                                                              <  1:371937/151‑1 (MQ=255)
                                                                    tGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGAtt                                                                          >  1:467696/1‑151 (MQ=255)
                                                                     ggCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGAtt                                                                          <  2:510112/150‑1 (MQ=255)
                                                                      gCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGAttt                                                                         >  2:11905/1‑150 (MQ=255)
                                                                         atatTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGCGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACa                                                                      >  2:579278/1‑150 (MQ=255)
                                                                             tGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCg                                                                  <  2:413365/150‑1 (MQ=255)
                                                                              gcgcAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCgg                                                                 <  1:279127/150‑1 (MQ=255)
                                                                                    tGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATaa                                                           >  2:494825/1‑150 (MQ=255)
                                                                                     ggATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATaaa                                                          <  1:666214/150‑1 (MQ=255)
                                                                                      gATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATaaa                                                          >  2:589190/1‑149 (MQ=255)
                                                                                      gATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAg                                                        <  1:561428/151‑1 (MQ=255)
                                                                                        ttGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGcc                                                      >  1:230402/1‑151 (MQ=255)
                                                                                            aCGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTg                                                   >  1:636928/1‑150 (MQ=255)
                                                                                                   cTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATg                                            >  2:629303/1‑150 (MQ=255)
                                                                                                     gatgaGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGa                                           <  1:567806/149‑1 (MQ=255)
                                                                                                        gaGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGat                                      <  2:406931/151‑1 (MQ=255)
                                                                                                          gCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGata                                     <  2:90214/150‑1 (MQ=255)
                                                                                                              aCATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCCGAAAGATGAGTGATATCAGt                                <  2:78949/151‑1 (MQ=255)
                                                                                                                aTGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATCAGTa                               >  2:241236/1‑150 (MQ=255)
                                                                                                                 tGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATCAGTa                               >  2:51404/1‑149 (MQ=255)
                                                                                                                      aGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATCAGTAAGGCGAg                        <  1:466766/151‑1 (MQ=255)
                                                                                                                                ggCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATCAGTAAGGCGAGCCTGCCTa                >  2:471571/1‑149 (MQ=255)
                                                                                                                                   acaGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATCAGTAAGGCGAGCCTGCCTAAGGCg           <  2:36089/151‑1 (MQ=255)
                                                                                                                                   acaGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATCAGTAAGGCGAGCCTGCCTAAGGCg           >  1:455096/1‑151 (MQ=255)
                                                                                                                                          aTAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATCAGTAAGGCGAGCCTGCCTAAGGCGAtttttt    <  1:505956/151‑1 (MQ=255)
                                                                                                                                            aaCCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATCAGTAAGGCGAGCCTGCCTAAGGCGATTTTTTTg  >  2:400866/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATCAGTAAGGCGAGCCTGCCTAAGGCGATTTTTTTG  >  NC_000913/4399005‑4399296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A