Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,399,151	Δ1 bp	coding (1740/1848 nt)	mutL →	methyl‑directed mismatch repair protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,399,151	0	G	.	97.6%	188.6 / ‑1.3	41	coding (1740/1848 nt)	mutL	methyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand): ref base G (0/1); new base . (17/23); total (17/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

CAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATCAGTAAGGCGAGCCTGCCTAAGGCGATTTTTTTG  >  NC_000913/4399005‑4399296

caacaaAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGc                                                                                                                                              >  2:234084/1‑151 (MQ=255)

  acaaAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCg                                                                                                                                             <  1:91790/151‑1 (MQ=255)

  acaaAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCgg                                                                                                                                            <  2:581628/151‑1 (MQ=255)

     aaaTTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGa                                                                                                                                           <  1:44434/149‑1 (MQ=255)

     aaaTTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACg                                                                                                                                         <  2:475775/151‑1 (MQ=255)

      aaTTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGt                                                                                                                                        >  1:599125/1‑151 (MQ=255)

          tACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGa                                                                                                                                     >  2:422608/1‑150 (MQ=255)

                    gATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGt                                                                                                                           <  2:325685/150‑1 (MQ=255)

                    gATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGt                                                                                                                           <  1:66294/150‑1 (MQ=255)

                     aTTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTcc                                                                                                                         >  1:547443/1‑151 (MQ=255)

                      ttCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCg                                                                                                                        >  2:30730/1‑151 (MQ=255)

                       tCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCg                                                                                                                        <  2:493054/150‑1 (MQ=255)

                            aaCTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACtt                                                                                                                  <  2:370334/151‑1 (MQ=255)

                            aaCTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACtt                                                                                                                  >  2:614294/1‑151 (MQ=255)

                                        aCCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAAcgcc                                                                                                       <  2:356895/150‑1 (MQ=255)

                                        aCCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAAcgc                                                                                                        <  2:488601/149‑1 (MQ=255)

                                           tGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGccgccg                                                                                                   >  1:205256/1‑151 (MQ=255)

                                                      tCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGtt                                                                                        >  1:370733/1‑151 (MQ=255)

                                                              cGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTg                                                                                <  1:10889/151‑1 (MQ=255)

                                                              cGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTg                                                                                <  1:422615/151‑1 (MQ=255)

                                                               gAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGt                                                                               >  1:479618/1‑151 (MQ=255)

                                                                 aCCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTg                                                                             >  1:268201/1‑151 (MQ=255)

                                                                      gCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGAttt                                                                         >  2:553850/1‑150 (MQ=255)

                                                                        aaTATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTAc                                                                       >  2:255682/1‑150 (MQ=255)

                                                                        aaTATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACa                                                                      <  1:345482/151‑1 (MQ=255)

                                                                          tatTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATc                                                                    >  2:592418/1‑151 (MQ=255)

                                                                           atTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATc                                                                    <  1:385908/150‑1 (MQ=255)

                                                                               cgcAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCg                                                               <  1:390125/151‑1 (MQ=255)

                                                                                gcAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCg                                                               >  1:373222/1‑150 (MQ=255)

                                                                                gcAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGa                                                              <  1:566338/151‑1 (MQ=255)

                                                                                   gTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATa                                                            <  2:140602/150‑1 (MQ=255)

                                                                                     ggATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATaaaa                                                         <  2:571529/151‑1 (MQ=255)

                                                                                          gCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCt                                                    >  2:630869/1‑151 (MQ=255)

                                                                                              gAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGaaa                                                <  1:625977/151‑1 (MQ=255)

                                                                                                aaTCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGa                                              <  1:185512/151‑1 (MQ=255)

                                                                                                     gatgaGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAg                                          <  1:586743/150‑1 (MQ=255)

                                                                                                       tgaGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTg                                        >  1:58626/1‑150 (MQ=255)

                                                                                                        gaGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGat                                      <  2:583123/151‑1 (MQ=255)

                                                                                                                aTGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATGTCAGTaa                              <  1:490443/151‑1 (MQ=255)

                                                                                                                                  cacaGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATCAGTAAGGCGAGCCTGCCTAAGGc            <  1:614311/151‑1 (MQ=255)

                                                                                                                                            aaCCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATCAGTAAGGCGAGCCTGCCTAAGGCGATTTTTTTg  >  2:523678/1‑151 (MQ=255)

CAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATCAGTAAGGCGAGCCTGCCTAAGGCGATTTTTTTG  >  NC_000913/4399005‑4399296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A