Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 715 38.4 1606192 96.6% 1551581 120.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,913,699 C→T *434* (TAG→TAA)  rlmD ← 23S rRNA m(5)U1939 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,913,6990CT100.0% 187.4 / NA 58*434* (TAG→TAA) rlmD23S rRNA m(5)U1939 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/26);  total (32/26)

GCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCAGGGTTA  >  NC_000913/2913568‑2913834
                                                                                                                                   |                                                                                                                                       
gctggTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTaa                                                                                                                                  >  1:309395/1‑139 (MQ=255)
    gTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAAcgcg                                                                                                                              >  1:402811/1‑139 (MQ=255)
              gACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGt                                                                                                                    >  2:708817/1‑139 (MQ=255)
               aCTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTa                                                                                                                   >  1:703209/1‑139 (MQ=255)
                  tGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCa                                                                                                                >  2:603257/1‑139 (MQ=255)
                    cGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATc                                                                                                              >  1:748709/1‑139 (MQ=255)
                       tCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGAt                                                                                                           <  2:748709/139‑1 (MQ=255)
                         cATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTc                                                                                                         >  1:433205/1‑139 (MQ=255)
                             tttCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGa                                                                                                     <  1:93227/139‑1 (MQ=255)
                                    aTCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCg                                                                                              >  2:724178/1‑139 (MQ=255)
                                         aTTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCgtgtgt                                                                                         <  2:644142/139‑1 (MQ=255)
                                              ccAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGaa                                                                                    >  2:315856/1‑139 (MQ=255)
                                               cAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAAc                                                                                   <  2:433205/139‑1 (MQ=255)
                                                 gCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACat                                                                                 >  1:91464/1‑139 (MQ=255)
                                                     tATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATcc                                                                             >  2:329566/1‑139 (MQ=255)
                                                      aTTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCa                                                                            <  2:668482/139‑1 (MQ=255)
                                                            atGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCg                                                                      >  1:276370/1‑139 (MQ=255)
                                                             tgtgCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGc                                                                     >  2:357538/1‑139 (MQ=255)
                                                               tgCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCa                                                                   >  2:694929/1‑139 (MQ=255)
                                                               tgCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCa                                                                   >  2:712016/1‑139 (MQ=255)
                                                               tgCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCa                                                                   >  2:343103/1‑139 (MQ=255)
                                                                 cACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTcc                                                                                                <  2:501670/108‑1 (MQ=255)
                                                                 cACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGt                                                                 >  1:126315/1‑139 (MQ=255)
                                                                 cACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGt                                                                 >  1:19777/1‑139 (MQ=255)
                                                                 cACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATGATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTcc                                                                                                >  1:501670/1‑108 (MQ=255)
                                                                   actctaACCACAACCGCCGTCCCCTCCTTTGAGGCCCTGGCATGTCGAAGTCGGACACACCCGATTAGTGAACTAGCGACAAAAGGACCGCCAAATCCAAATGCCCCGTGTGTGGGAACATAGCCAGCATCGCCAGTcg                                                               <  1:603257/137‑1 (MQ=255)
                                                                    ttcttACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGAt                                                                                                           <  1:222157/94‑1 (MQ=255)
                                                                    ttcttACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGAt                                                                                                           >  2:222157/1‑94 (MQ=255)
                                                                     tcttACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTcgcg                                                             <  2:91464/139‑1 (MQ=255)
                                                                      cttACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCgcgc                                                            <  2:48912/139‑1 (MQ=255)
                                                                       ttACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCa                                                           <  2:276370/139‑1 (MQ=255)
                                                                       ttACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCa                                                           <  1:448241/139‑1 (MQ=255)
                                                                        tACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCaa                                                          >  2:612019/1‑139 (MQ=255)
                                                                         aCCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAAt                                                         >  1:327242/1‑139 (MQ=255)
                                                                         aCCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAAt                                                         >  2:514803/1‑139 (MQ=255)
                                                                            gCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGt                                                      >  1:267811/1‑139 (MQ=255)
                                                                              aaCCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCa                                                           >  1:294699/1‑132 (MQ=255)
                                                                              aaCCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCa                                                           <  2:294699/132‑1 (MQ=255)
                                                                              aaCCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCa                                                           >  1:576858/1‑132 (MQ=255)
                                                                              aaCCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCa                                                           <  2:576858/132‑1 (MQ=255)
                                                                              aaCCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGtat                                                    >  1:788977/1‑139 (MQ=255)
                                                                                ccATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACatat                                                                               >  2:669317/1‑110 (MQ=255)
                                                                                ccATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACatat                                                                               <  1:669317/110‑1 (MQ=255)
                                                                                                tAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTAAATCCTGCTTTTAATAACg                                  <  2:327242/139‑1 (MQ=255)
                                                                                                   ggACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGCTGTCCCg                                                                                              >  1:119040/1‑76 (MQ=255)
                                                                                                   ggACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCg                                                                                              <  2:119040/76‑1 (MQ=255)
                                                                                                    gACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTc                              >  2:66499/1‑139 (MQ=255)
                                                                                                      ccAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGc                            <  1:211970/139‑1 (MQ=255)
                                                                                                       cAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGc                                             <  2:243012/121‑1 (MQ=255)
                                                                                                       cAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGc                                             >  1:243012/1‑121 (MQ=255)
                                                                                                          aCCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATc                        >  2:592451/1‑139 (MQ=255)
                                                                                                                   aaTCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAg               <  1:66499/139‑1 (MQ=255)
                                                                                                                   aaTCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAg               <  2:126315/139‑1 (MQ=255)
                                                                                                                      cGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGt            <  2:267811/139‑1 (MQ=255)
                                                                                                                          aaaTCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCa        <  2:102064/139‑1 (MQ=255)
                                                                                                                                cAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCAGGGTTa  <  1:559498/139‑1 (MQ=255)
                                                                                                                                cAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCAGGGTTa  <  1:342835/139‑1 (MQ=255)
                                                                                                                                cAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCAGGGTTa  <  2:732734/139‑1 (MQ=255)
                                                                                                                                cAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCAGTGTGTGGGAACATATCTAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCAGGTTTa  <  1:514803/139‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                       
GCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCAGGGTTA  >  NC_000913/2913568‑2913834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A