Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I2 R1	772	77.0	3769570	91.6%	3452926	105.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,913,699	C→T	434 (TAG→TAA)	rlmD ←	23S rRNA m(5)U1939 methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,913,699	0	C	T	99.0%	307.0 / ‑3.9	95	434 (TAG→TAA)	rlmD	23S rRNA m(5)U1939 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (43/51); minor base A (1/0); total (44/51)
Fisher's exact test for biased strand distribution p-value = 4.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

TAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAAT‑GGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTG  >  NC_000913/2913573‑2913826

tAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAAcgcgc                                                                                                                      <  2:240011/139‑1 (MQ=255)

 aaTACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAAcgcgcg                                                                                                                     <  2:1341578/139‑1 (MQ=255)

     cccAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGaa                                                                                                                 <  1:1298062/139‑1 (MQ=255)

          aCTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTa                                                                                                            >  1:1041126/1‑139 (MQ=255)

          aCTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTa                                                                                                            >  2:1563388/1‑139 (MQ=255)

          aCTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTa                                                                                                            <  2:1724218/139‑1 (MQ=255)

              gCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCga                                                                                                                    >  2:862161/1‑127 (MQ=255)

              gCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCga                                                                                                                    <  1:862161/127‑1 (MQ=255)

              gCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCAt                                                                                                        <  2:1743743/139‑1 (MQ=255)

               cGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTACACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATc                                                                                                       >  1:1154155/1‑139 (MQ=255)

                    cATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCAt                                                                                                        >  1:1095665/1‑133 (MQ=255)

                    cATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCAt                                                                                                        <  2:1095665/133‑1 (MQ=255)

                       ttttCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAg                                                                                               <  2:633757/139‑1 (MQ=255)

                            cGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATGGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTc                                                                                          >  1:155825/1‑139 (MQ=255)

                              gATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGAt                                                                                                    >  1:506423/1‑127 (MQ=255)

                              gATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGAt                                                                                                    <  2:506423/127‑1 (MQ=255)

                                tCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCgt                                                                                      <  2:155825/139‑1 (MQ=255)

                                    aTTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCgtgtgt                                                                                  >  1:675897/1‑139 (MQ=255)

                                         ccAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGaa                                                                             <  1:1563388/139‑1 (MQ=255)

                                          cAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAAc                                                                            <  2:894316/139‑1 (MQ=255)

                                                 aTTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCa                                                                     >  1:1049634/1‑139 (MQ=255)

                                                       atGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTaa                                                                                                                           >  1:1043338/1‑79 (MQ=255)

                                                       atGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTaa                                                                                                                           <  2:1043338/79‑1 (MQ=255)

                                                       atGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCgtg                                                                                     >  2:1303493/1‑117 (MQ=255)

                                                       atGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCgtg                                                                                     <  1:1303493/117‑1 (MQ=255)

                                                       atGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCg                                                               >  1:247216/1‑139 (MQ=255)

                                                         gtgCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGtgtgtg                                                                                 <  1:1563086/119‑1 (MQ=255)

                                                         gtgCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGtgtgtg                                                                                 >  2:1563086/1‑119 (MQ=255)

                                                          tgCACTTCTTACCGCCACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACata                                                                         <  2:1128047/126‑1 (MQ=255)

                                                          tgCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTc                                                                                                  <  2:875545/101‑1 (MQ=255)

                                                          tgCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTc                                                                                                  >  1:875545/1‑101 (MQ=255)

                                                          tccACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACata                                                                         >  1:1128047/3‑126 (MQ=255)

                                                            cACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGt                                                          >  2:1857179/1‑139 (MQ=255)

                                                            cACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGt                                                          >  1:986583/1‑139 (MQ=255)

                                                            cACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGt                                                          >  2:421909/1‑139 (MQ=255)

                                                                tcttACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTcgcg                                                      <  2:247216/139‑1 (MQ=255)

                                                                tcttACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTcgcg                                                      <  2:1041126/139‑1 (MQ=255)

                                                                tcttACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTcgcg                                                      <  1:1857179/139‑1 (MQ=255)

                                                                tcttACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTcgcg                                                      <  1:421909/139‑1 (MQ=255)

                                                                tcttACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTcgcg                                                      <  2:675897/139‑1 (MQ=255)

                                                                 cttACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGAt                                                                                                    >  2:1680820/1‑92 (MQ=255)

                                                                 cttACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGAt                                                                                                    <  1:1680820/92‑1 (MQ=255)

                                                                 cttACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAAATATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCgcgc                                                     >  2:1348907/1‑139 (MQ=255)

                                                                  ttACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCa                                                    <  2:986583/139‑1 (MQ=255)

                                                                  ttACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCa                                                    <  2:35649/139‑1 (MQ=255)

                                                                    aCCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTc                                                                                                  <  2:727808/91‑1 (MQ=255)

                                                                    aCCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTc                                                                                                  >  1:727808/1‑91 (MQ=255)

                                                                         aaCCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGAt                                                                                                    <  1:251075/84‑1 (MQ=255)

                                                                         aaCCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGAt                                                                                                    >  2:251075/1‑84 (MQ=255)

                                                                           ccATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGa                                                                              >  1:1211496/1‑104 (MQ=255)

                                                                           ccATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGa                                                                              <  2:1211496/104‑1 (MQ=255)

                                                                           ccATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAAT‑gggtata                                          >  2:687902/1‑134 (MQ=255)

                                                                              tCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAAT‑GGTATATCCt                                       >  2:1777779/1‑139 (MQ=255)

                                                                                gTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTa                                                                                                            >  2:930404/1‑69 (MQ=255)

                                                                                gTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTa                                                                                                            <  1:930404/69‑1 (MQ=255)

                                                                                      tcCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAAT‑GGTATATCCTGCTTTtaa                               >  1:205964/1‑139 (MQ=255)

                                                                                      tcCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAAT‑GGTATATCCTGCTTTtaa                               >  2:1324911/1‑139 (MQ=255)

                                                                                           tAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAAcgcg                                                                                                                       >  1:1196595/1‑47 (MQ=255)

                                                                                           tAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAAcgcg                                                                                                                       <  2:1196595/47‑1 (MQ=255)

                                                                                           tAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAAT‑GGTATATCCTGCTTTTAATAACg                          <  1:833038/139‑1 (MQ=255)

                                                                                           tAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAAT‑GGTATATCCTGCTTTTAATAACg                          <  2:1454697/139‑1 (MQ=255)

                                                                                                aCCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCgtgtg                                                                                   <  1:612881/78‑1 (MQ=255)

                                                                                                aCCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCgtgtg                                                                                   >  2:612881/1‑78 (MQ=255)

                                                                                                aCCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTggg                                                                               <  2:510058/82‑1 (MQ=255)

                                                                                                aCCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTggg                                                                               >  1:510058/1‑82 (MQ=255)

                                                                                                 ccAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGGTATATCCTGCTTTTAATAACGCTTCg                     <  1:687902/139‑1 (MQ=255)

                                                                                                  cAGACCTGCCGAAATCGGCAAATCGCAATTATTTaa                                                                                                                           >  1:664796/1‑36 (MQ=255)

                                                                                                  cAGACCTGCCGAAATCGGCAAATCGCAATTATTTaa                                                                                                                           <  2:664796/36‑1 (MQ=255)

                                                                                                  cAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGt                                                                                                             >  1:1256345/1‑50 (MQ=255)

                                                                                                  cAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGt                                                                                                             <  2:1256345/50‑1 (MQ=255)

                                                                                                  cAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTAccc                                                                                                         <  2:1155322/54‑2 (MQ=255)

                                                                                                  cAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCa                                                                                                         >  1:1155322/1‑54 (MQ=255)

                                                                                                  cAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCAt                                                                                                        >  2:1754111/1‑55 (MQ=255)

                                                                                                  cAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCAt                                                                                                        <  1:1754111/55‑1 (MQ=255)

                                                                                                  cAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGa                                                                                                     <  1:583438/58‑1 (MQ=255)

                                                                                                  cAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGa                                                                                                     >  2:583438/1‑58 (MQ=255)

                                                                                                  cAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGtgtgtg                                                                                 >  1:365059/1‑78 (MQ=255)

                                                                                                  cAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGtgtgtg                                                                                 <  2:365059/78‑1 (MQ=255)

                                                                                                  cAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTcg                                                        <  2:1184549/103‑1 (MQ=255)

                                                                                                  cAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTcg                                                        >  1:1184549/1‑103 (MQ=255)

                                                                                                     aCCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCgt                                                                                      <  2:1212030/70‑1 (MQ=255)

                                                                                                     aCCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGACCATATCCAGCATCGCCAGTCGCGCa                                                    >  2:1763149/1‑104 (MQ=255)

                                                                                                     aCCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCa                                                    <  1:1763149/104‑1 (MQ=255)

                                                                                                     aCCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAAT‑GGTATATCCTGCTTTTAATAACg                          <  2:1791699/129‑1 (MQ=255)

                                                                                                     aCCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAAT‑GGTATATCCTGCTTTTAATAACg                          >  1:1791699/1‑129 (MQ=255)

                                                                                                     aCCCGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCgt                                                                                      >  1:1212030/1‑70 (MQ=255)

                                                                                                      ccTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAAT‑GGTATATCCTGCTTTTAATAACGCTTCGCTATcc               <  2:205964/139‑1 (MQ=255)

                                                                                                          ccGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTcgcg                                                      >  1:1611561/1‑97 (MQ=255)

                                                                                                          ccGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTcgcg                                                      <  2:1611561/97‑1 (MQ=255)

                                                                                                          ccGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAAT‑GGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAg           >  2:508345/1‑139 (MQ=255)

                                                                                                           cGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAAT‑GGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGc          <  1:1324911/139‑1 (MQ=255)

                                                                                                                   gCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAAT‑GGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTg  <  2:1046540/139‑1 (MQ=255)

                                                                                                                   gCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAAT‑GGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTg  <  1:860296/139‑1 (MQ=255)

                                                                                                                    cAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGAt                                                                                                    <  1:97953/41‑1 (MQ=255)

                                                                                                                    cAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGAt                                                                                                    >  2:97953/1‑41 (MQ=255)

TAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAAT‑GGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTG  >  NC_000913/2913573‑2913826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A