Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I2 R1	777	59.2	2955938	92.5%	2734242	106.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,856,453	C→T	118 (TAG→TAA)	ygbA ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,856,453	0	C	T	100.0%	112.3 / NA	35	118 (TAG→TAA)	ygbA	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (15/20); total (15/20)

CTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATTCATGAGGTTTTTT‑CGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCT  >  NC_000913/2856345‑2856583

cTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTATT‑CGGACGATAtttt                                                                                                      >  2:984605/1‑139 (MQ=255)

      cTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTT‑CGGACGATATTTTTCCGGc                                                                                                <  2:812030/139‑1 (MQ=255)

      cTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTT‑CGGACGATATTTTTCCGGc                                                                                                <  2:654192/139‑1 (MQ=255)

                   cccTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTT‑CGGACGATATTTTTCCGGCAGTTCTGGCACCg                                                                                   >  2:1127298/1‑139 (MQ=255)

                     ctgctgTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGTCAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTT‑CGGACGATATTTTTCCGGGACTTCTGGCACCGGa                                                                                 <  1:984605/139‑1 (MQ=255)

                            cACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTT‑CGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGt                                                                          <  2:336774/139‑1 (MQ=255)

                             aCGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTT‑CGGACGATATTTTTCCGGCAGTTCTGGCACCgta                                                                                 >  1:869121/1‑129 (MQ=255)

                             aCGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTT‑CGGACGATATTTTTCCGGCAGTTCTGGCACCGGa                                                                                 <  2:869121/131‑1 (MQ=255)

                             aCGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTT‑CGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTc                                                                         <  1:605368/139‑1 (MQ=255)

                                  tGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTT‑CGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCga                                                                    >  1:1461263/1‑139 (MQ=255)

                                            gggAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTT‑CGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACg                                                          <  2:495351/139‑1 (MQ=255)

                                            gggAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTT‑CGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACg                                                          <  2:678478/139‑1 (MQ=255)

                                             ggAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTT‑CGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGc                                                         <  2:1461263/139‑1 (MQ=255)

                                                  tGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTT‑CGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGt                                                    <  1:1328890/139‑1 (MQ=255)

                                                       aaTCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTT‑CGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCga                                                                    <  2:676042/118‑1 (MQ=255)

                                                       aaTCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTT‑CGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCga                                                                    >  1:676042/1‑118 (MQ=255)

                                                         tCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTT‑CGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATc                                             >  2:542724/1‑139 (MQ=255)

                                                            gCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTT‑CGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGa                                          <  1:542724/139‑1 (MQ=255)

                                                                 gATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTT‑CGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACg                                     >  2:930478/1‑139 (MQ=255)

                                                                       aCTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTT‑CGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCga                                                                    <  2:1275935/102‑1 (MQ=255)

                                                                       aCTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTT‑CGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCga                                                                    >  1:1275935/1‑102 (MQ=255)

                                                                          gCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTT‑CGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAg                               <  2:1325720/136‑1 (MQ=255)

                                                                          gCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTT‑CGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAg                               >  1:1325720/1‑136 (MQ=255)

                                                                                        aGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTT‑CGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCggcccggccc              <  1:1127298/139‑1 (MQ=255)

                                                                                            cTGCCTGTCCGGCATATTATTCATGAGGTTTTTT‑CGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACg          <  2:466612/139‑1 (MQ=255)

                                                                                               ccTGTCCGGCATATTATTCATGAGGTTTTTT‑CGGACGATATTTTTCCGGc                                                                                                >  2:1288772/1‑50 (MQ=255)

                                                                                               ccTGTCCGGCATATTATTCATGAGGTTTTTT‑CGGACGATATTTTTCCGGc                                                                                                <  1:1288772/50‑1 (MQ=255)

                                                                                               ccTGTCCGGCATATTATTCATGAGGTTTTTT‑CGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCAt       >  2:1408171/1‑139 (MQ=255)

                                                                                                cTGTCCGGCATATTATTCATGAGGTTTTTT‑CGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGAt                                         <  2:143866/104‑1 (MQ=255)

                                                                                                cTGTCCGGCATATTATTCATGAGGTTTTTT‑CGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGAt                                         >  1:143866/1‑104 (MQ=255)

                                                                                                  gTCCGGCATATTATTCATGAGGTTTTTTCCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGc                                                         <  1:1123663/87‑1 (MQ=255)

                                                                                                  gTCCGGCATATTATTCATGAGGTTTTTTCCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGc                                                         >  2:1123663/1‑87 (MQ=255)

                                                                                                    ccGGCATATTATTCATGAGGTTTTTT‑CGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGc                                                         >  1:916254/1‑84 (MQ=255)

                                                                                                    ccGGCATATTATTCATGAGGTTTTTT‑CGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGc                                                         <  2:916254/84‑1 (MQ=255)

                                                                                                    ccGGCATATTATTCATGAGGTTTTTT‑CGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCt  >  2:1094175/1‑139 (MQ=255)

CTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATTCATGAGGTTTTTT‑CGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCT  >  NC_000913/2856345‑2856583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A