Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F1 I1 R1
|
778 |
68.7 |
3228504 |
93.4% |
3015422 |
109.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,856,453 |
C→T |
*118* (TAG→TAA) |
ygbA ← |
uncharacterized protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,856,453 | 0 | C | T | 100.0%
| 137.9
/ NA
| 44 | *118* (TAG→TAA) | ygbA | uncharacterized protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (26/18); total (26/18) |
AACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCC‑‑‑‑‑GGACAGATCTGCCTGTCCGGCATACTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCT > NC_000913/2856340‑2856583
|
taCGTCTGCGGCTGAAACGCACGACCCTCCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCC‑‑‑‑‑GGACAGATCTGCCTTTCCGGCATATTATTCATGAGGTTTTTTCGGACAat < 1:1221468/138‑1 (MQ=255)
aaaCGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCC‑‑‑‑‑GGACAGATCTGCCTGTCCGGCATATTATTCAt > 2:443045/1‑107 (MQ=255)
aaaCGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCC‑‑‑‑‑GGACAGATCTGCCTGTCCGGCATATTATTCAt < 1:443045/107‑1 (MQ=255)
aCGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGc > 2:1279894/1‑139 (MQ=255)
aCGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCC‑‑‑‑‑GGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTc > 1:1143866/1‑139 (MQ=255)
tGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCC‑‑‑‑‑GGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTc > 1:1329902/1‑107 (MQ=255)
tGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCC‑‑‑‑‑GGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTc < 2:1329902/107‑1 (MQ=255)
tGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCC‑‑‑‑‑GGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTc < 2:70134/134‑1 (MQ=255)
tGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCC‑‑‑‑‑GGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTc > 1:70134/1‑134 (MQ=255)
tGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCC‑‑‑‑‑GGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCga > 1:393710/1‑139 (MQ=255)
ggAATTGATAAATCGGCATTGATTTAACTGCAAATTGCC‑‑‑‑‑GGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGc < 2:1261015/139‑1 (MQ=255)
aaTTGATAAATCGGCATTGATTTAACTGCAAATTGCC‑‑‑‑‑GGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCgatg < 2:589314/128‑1 (MQ=255)
aaTTGATAAATCGGCATTGATTTAACTGCAAATTGCC‑‑‑‑‑GGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCgatg > 1:589314/1‑128 (MQ=255)
aTAAATCGGCATTGATTTAACTGCAAATTGCC‑‑‑‑‑GGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGc < 1:1005917/93‑1 (MQ=255)
aTAAATCGGCATTGATTTAACTGCAAATTGCC‑‑‑‑‑GGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGc > 2:1005917/1‑93 (MQ=255)
aTCGGCATTGATTTAACTGCAAATTGCC‑‑‑‑‑GGACAGATCTGCCTGTCCGGCATATTATTCAt < 1:498519/60‑1 (MQ=255)
aTCGGCATTGATTTAACTGCAAATTGCC‑‑‑‑‑GGACAGATCTGCCTGTCCGGCATATTATTCAt > 2:498519/1‑60 (MQ=255)
tCGGCATTGATTTAACTGCAAATTGCC‑‑‑‑‑GGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCgg > 2:785378/1‑87 (MQ=255)
tCGGCATTGATTTAACTGCAAATTGCC‑‑‑‑‑GGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCgg < 1:785378/87‑1 (MQ=255)
gCATTGATTTAACTGCAAATTGCC‑‑‑‑‑GGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGa > 1:1478783/1‑139 (MQ=255)
gCATTGATTTAACTGCAAATTGCC‑‑‑‑‑GGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGa > 2:1151761/1‑139 (MQ=255)
aTTTAACTGCAAATTGCC‑‑‑‑‑GGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGc < 1:1488703/79‑1 (MQ=255)
aTTTAACTGCAAATTGCC‑‑‑‑‑GGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTGTCGGACGATATTTTTCCGGc > 2:1488703/1‑79 (MQ=255)
aCTGCAAATTGCC‑‑‑‑‑GGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCg > 1:566007/1‑72 (MQ=255)
aCTGCAAATTGCC‑‑‑‑‑GGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCg < 2:566007/72‑1 (MQ=255)
aCTGCAAATTGCC‑‑‑‑‑GGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCg > 2:974295/1‑101 (MQ=255)
aCTGCAAATTGCC‑‑‑‑‑GGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCg < 1:974295/101‑1 (MQ=255)
cAAATTGCC‑‑‑‑‑GGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCAtt > 1:1218016/1‑139 (MQ=255)
aaaTTGCC‑‑‑‑‑GGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATtc > 2:1291666/1‑139 (MQ=255)
cc‑‑‑‑‑GGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACg > 1:1057203/1‑101 (MQ=255)
cc‑‑‑‑‑GGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACg < 2:1057203/101‑1 (MQ=255)
aGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCggcccggccc < 1:1279894/139‑1 (MQ=255)
cTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACg > 1:1537238/1‑139 (MQ=255)
tGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGc > 2:448982/1‑139 (MQ=255)
gTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTcggcccggcc < 2:992072/128‑1 (MQ=255)
gTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTcggcccggcc > 1:992072/1‑128 (MQ=255)
ccGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCggcccggccc < 1:906168/127‑1 (MQ=255)
ccGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCggcccggccc > 2:906168/1‑127 (MQ=255)
ccGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCt > 2:1098818/1‑139 (MQ=255)
ccGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCt > 1:1094271/1‑139 (MQ=255)
cGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACg > 2:465570/1‑82 (MQ=255)
cGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACg < 1:465570/82‑1 (MQ=255)
cGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCggcccggccc > 2:44220/1‑126 (MQ=255)
cGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCggcccggccc < 1:44220/126‑1 (MQ=255)
|
AACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCC‑‑‑‑‑GGACAGATCTGCCTGTCCGGCATACTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCT > NC_000913/2856340‑2856583
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
