Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F1 I1 R1
|
770 |
68.5 |
3470224 |
91.7% |
3182195 |
103.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,856,453 |
C→T |
*118* (TAG→TAA) |
ygbA ← |
uncharacterized protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,856,453 | 0 | C | T | 100.0%
| 153.3
/ NA
| 48 | *118* (TAG→TAA) | ygbA | uncharacterized protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (22/26); total (22/26) |
AAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCT > NC_000913/2856328‑2856583
|
aaGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGtt > 1:184055/1‑139 (MQ=255)
gcgcAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCa < 2:781347/124‑1 (MQ=255)
gcgcAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCa > 1:781347/1‑124 (MQ=255)
gcgcAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGa < 1:1400933/139‑1 (MQ=255)
aaaCGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGt > 1:1440386/1‑139 (MQ=255)
cACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGt < 2:1318232/139‑1 (MQ=255)
aTGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAgg > 1:1477541/1‑87 (MQ=255)
aTGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAgg < 2:1477541/87‑1 (MQ=255)
gCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATAtttt > 2:1469629/1‑101 (MQ=255)
gCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATAtttt < 1:1469629/101‑1 (MQ=255)
gCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCgatgag < 1:289729/139‑1 (MQ=255)
gggAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACg < 2:1440386/139‑1 (MQ=255)
gggAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACg < 2:1120205/139‑1 (MQ=255)
tGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCg < 1:80168/78‑1 (MQ=255)
tGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCg > 2:80168/1‑78 (MQ=255)
tGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGt < 2:1007155/139‑1 (MQ=255)
aaTCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTc < 2:1551922/113‑1 (MQ=255)
aaTCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTc > 1:1551922/1‑113 (MQ=255)
aaTCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACg > 1:1681195/1‑128 (MQ=255)
aaTCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACg < 2:1681195/128‑1 (MQ=255)
aTCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCAcc < 1:68765/101‑1 (MQ=255)
aTCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCAcc > 2:68765/1‑101 (MQ=255)
aTTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGc > 1:662680/1‑139 (MQ=255)
aaCTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCt < 2:1163953/94‑1 (MQ=255)
aaCTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCcaca > 2:1619658/1‑139 (MQ=255)
aaCTGCAAAATGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCt > 1:1163953/1‑94 (MQ=255)
aaaTTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATtc > 1:905740/1‑139 (MQ=255)
aaTTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATtct < 1:1619658/139‑1 (MQ=255)
ccGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTcggcc < 2:905740/139‑1 (MQ=255)
cGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTcggccc < 2:43104/139‑1 (MQ=255)
aTCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTc > 2:784458/1‑60 (MQ=255)
aTCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTc < 1:784458/60‑1 (MQ=255)
aTCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTcgg > 2:700085/1‑129 (MQ=255)
aTCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTcgg < 1:700085/129‑1 (MQ=255)
ccTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGc < 2:1668855/89‑1 (MQ=255)
ccTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGc > 1:1668855/1‑89 (MQ=255)
gTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAAt < 2:662680/139‑1 (MQ=255)
tCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCgat < 2:1395948/75‑1 (MQ=255)
tCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCgat > 1:1395948/1‑75 (MQ=255)
cgggCATATTATTCAAGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCt > 2:1160612/3‑139 (MQ=255)
ccGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGtt < 2:136709/49‑1 (MQ=255)
ccGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGtt > 1:136709/1‑49 (MQ=255)
ccGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCAt > 1:146188/1‑70 (MQ=255)
ccGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCAt < 2:146188/70‑1 (MQ=255)
gCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCgat > 2:184/1‑71 (MQ=255)
gCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCgat < 1:184/71‑1 (MQ=255)
cATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCa < 2:1350876/51‑1 (MQ=255)
cATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCa > 1:1350876/1‑51 (MQ=255)
|
AAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCT > NC_000913/2856328‑2856583
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
