Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I1 R1	759	71.0	3673240	91.5%	3361014	104.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,856,453	C→T	118 (TAG→TAA)	ygbA ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,856,453	0	C	T	100.0%	146.4 / NA	47	118 (TAG→TAA)	ygbA	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (26/21); total (26/21)

CTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATC  >  NC_000913/2856334‑2856582

cTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCg                                                                                                                >  1:862564/1‑139 (MQ=255)

 tGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATCCATGAGGTTTTTTCgg                                                                                                               >  2:852311/1‑139 (MQ=255)

  gcgcAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGa                                                                                                              <  2:441718/139‑1 (MQ=255)

    gcAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACg                                                                                                            >  1:1056308/1‑139 (MQ=255)

                    aaaCGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGt                                                                                            >  2:1043867/1‑139 (MQ=255)

                     aaCGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGtt                                                                                           <  2:862564/139‑1 (MQ=255)

                                  gctgTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACg                                                                              <  2:1056308/139‑1 (MQ=255)

                                  gctgTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACg                                                                              >  2:1782145/1‑139 (MQ=255)

                                   ctgTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCa                                                                                     <  1:1011926/131‑1 (MQ=255)

                                   ctgTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCa                                                                                     >  2:1011926/1‑131 (MQ=255)

                                        aCGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTc                                                                        >  2:1392191/1‑139 (MQ=255)

                                        aCGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTc                                                                        >  2:1211640/1‑139 (MQ=255)

                                          ggATGAAGCGGCTGGGAATTGATAAATCGTCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCAt                                                                      <  1:852311/139‑1 (MQ=255)

                                                aGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCgg                                                                                                <  2:580203/107‑1 (MQ=255)

                                                aGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCgg                                                                                                >  1:580203/1‑107 (MQ=255)

                                                    gCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCga                                                                   >  1:1757498/1‑132 (MQ=255)

                                                    gCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCga                                                                   <  2:1757498/132‑1 (MQ=255)

                                                      tGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGAc                                                          >  1:1128950/1‑139 (MQ=255)

                                                      tGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGAc                                                          <  1:1392191/139‑1 (MQ=255)

                                                         gAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCa                                                       <  1:1211640/139‑1 (MQ=255)

                                                         gAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCa                                                       <  2:1128950/139‑1 (MQ=255)

                                                                  aaTCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGa                                              <  2:1145540/139‑1 (MQ=255)

                                                                    tCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACgg                                                    >  1:213501/1‑131 (MQ=255)

                                                                    tCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACgg                                                    <  2:213501/131‑1 (MQ=255)

                                                                    tCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATc                                            >  2:753032/1‑139 (MQ=255)

                                                                        cATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGAt                                        >  2:980856/1‑139 (MQ=255)

                                                                         aTTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCa                                                       <  2:1140868/123‑1 (MQ=255)

                                                                         aTTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCa                                                       >  1:1140868/1‑123 (MQ=255)

                                                                         aTTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATg                                       >  2:1808040/1‑139 (MQ=255)

                                                                          ttGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGa                                      <  1:980856/139‑1 (MQ=255)

                                                                                      cAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGcc                                  >  1:238475/1‑131 (MQ=255)

                                                                                      cAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGcc                                  <  2:238475/131‑1 (MQ=255)

                                                                                       aaaTTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATtc                         >  2:1812248/1‑139 (MQ=255)

                                                                                       aaaTTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATtc                         >  1:37561/1‑139 (MQ=255)

                                                                                        aaTTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATtct                        >  1:1135749/1‑139 (MQ=255)

                                                                                                gACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGa                                      <  1:369934/117‑1 (MQ=255)

                                                                                                gACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGa                                      >  2:369934/1‑117 (MQ=255)

                                                                                                     aTCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGt                                                   >  2:1605345/1‑99 (MQ=255)

                                                                                                     aTCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGt                                                   <  1:1605345/99‑1 (MQ=255)

                                                                                                     aTCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCaa           <  2:1135749/139‑1 (MQ=255)

                                                                                                          ccTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGa                                         <  1:412601/104‑1 (MQ=255)

                                                                                                          ccTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGa                                         >  2:412601/1‑104 (MQ=255)

                                                                                                             gTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGc                                                                             >  1:454486/1‑65 (MQ=255)

                                                                                                             gTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGc                                                                             <  2:454486/65‑1 (MQ=255)

                                                                                                              tCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATc  >  1:142606/1‑139 (MQ=255)

                                                                                                                cGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCg                                                                    <  2:842890/71‑1 (MQ=255)

                                                                                                                cGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCg                                                                    >  1:842890/1‑71 (MQ=255)

CTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATC  >  NC_000913/2856334‑2856582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A