Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I2 R1	766	64.2	3232166	90.9%	2938038	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,434,577	G→A	A122T (GCA→ACA)	fmt →	10‑formyltetrahydrofolate:L‑methionyl‑tRNA(fMet) N‑formyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,434,577	0	G	A	100.0%	206.1 / NA	63	A122T (GCA→ACA)	fmt	10‑formyltetrahydrofolate:L‑methionyl‑tRNA(fMet) N‑formyltransferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (28/35); total (28/35)

CGCCGAACTGCAGGCTGATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTGCACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAG  >  NC_000913/3434447‑3434697

cGCCGAACTGCAGGCTGATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATc                                                                                                                  >  2:1236505/1‑139 (MQ=255)

  ccGAACTGCAGGCTGATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCa                                                                                                                <  2:1444480/139‑1 (MQ=255)

      aCTGCAGGCTGATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGc                                                                                                            >  2:1144569/1‑139 (MQ=255)

      aCTGCAGGCTGATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGc                                                                                                            <  2:64294/139‑1 (MQ=255)

          cAGGCTGATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATcc                                                                                                                 <  2:557856/130‑1 (MQ=255)

          cAGGCTGATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATcc                                                                                                                 >  1:557856/1‑130 (MQ=255)

                 aTGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGc                                                                                                 <  1:1144569/139‑1 (MQ=255)

                         gtcgtcgtcGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGc                                                                                                 <  2:813421/131‑1 (MQ=255)

                         gtcgtcgtcGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGc                                                                                                 >  1:813421/1‑131 (MQ=255)

                          tcgtcgtcgCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGc                                                                                        <  2:300615/139‑1 (MQ=255)

                             tcgtcgCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGa                                                                                     >  1:1074892/1‑139 (MQ=255)

                             tcgtcgCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGa                                                                                     <  2:1262684/139‑1 (MQ=255)

                              cgtcgCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACg                                                                                                             >  2:1298929/1‑114 (MQ=255)

                              cgtcgCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACg                                                                                                             <  1:1298929/114‑1 (MQ=255)

                                   ccTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTgg                                                                               >  2:492263/1‑139 (MQ=255)

                                           ttAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCa                                                                       <  1:1236505/139‑1 (MQ=255)

                                               ttCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGt                                                                                             >  1:1015942/1‑113 (MQ=255)

                                               ttCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGt                                                                                             <  2:1015942/113‑1 (MQ=255)

                                                   gCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCaa                                                               >  2:429569/1‑139 (MQ=255)

                                                    ccGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCaaa                                                              <  1:1584987/139‑1 (MQ=255)

                                                        aaGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGa                                                          <  2:1023074/139‑1 (MQ=255)

                                                              tGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCa                                                                                                         >  1:1553222/1‑86 (MQ=255)

                                                              tGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCa                                                                                                         <  2:1553222/86‑1 (MQ=255)

                                                              tGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGgtgt                                                                            <  1:1336268/115‑1 (MQ=255)

                                                              tGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGgtgt                                                                            >  2:1336268/1‑115 (MQ=255)

                                                                    agaTGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTTCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGa                                              <  1:1173841/139‑1 (MQ=255)

                                                                       tGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGacac                                           <  2:524407/139‑1 (MQ=255)

                                                                       tGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGacac                                           <  1:492263/139‑1 (MQ=255)

                                                                        gCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCaa                                                                                                               <  1:765769/70‑1 (MQ=255)

                                                                        gCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCaa                                                                                                               >  2:765769/1‑70 (MQ=255)

                                                                        gCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGa                                                                                     <  2:531162/96‑1 (MQ=255)

                                                                        gCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGa                                                                                     >  1:531162/1‑96 (MQ=255)

                                                                         ccGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCg                                         <  2:1074892/139‑1 (MQ=255)

                                                                          cgcgTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTACTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCgg                                        >  2:351645/1‑139 (MQ=255)

                                                                                ttGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGatat                                  <  1:429569/139‑1 (MQ=255)

                                                                                     tGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTCAGACACCGGTGATATGctct                             <  1:567635/139‑1 (MQ=255)

                                                                                       tATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCaa                                                                                                               <  2:960979/55‑1 (MQ=255)

                                                                                       tATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCaa                                                                                                               >  1:960979/1‑55 (MQ=255)

                                                                                        aTCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCtata                          >  2:1121562/1‑139 (MQ=255)

                                                                                         tCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGt                                                       >  2:529815/1‑109 (MQ=255)

                                                                                         tCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGt                                                       <  1:529815/109‑1 (MQ=255)

                                                                                         tCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATaa                         >  2:508050/1‑139 (MQ=255)

                                                                                          cAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTACTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAg                        <  1:351645/139‑1 (MQ=255)

                                                                                             cGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTa                                                                                                      <  1:1028184/58‑1 (MQ=255)

                                                                                             cGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTa                                                                                                      >  2:1028184/1‑58 (MQ=255)

                                                                                             cGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCggg                                                                                              <  2:551668/66‑1 (MQ=255)

                                                                                             cGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCggg                                                                                              >  1:551668/1‑66 (MQ=255)

                                                                                             cGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGtgtg                                                                           >  2:206519/1‑85 (MQ=255)

                                                                                             cGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGtgtg                                                                           <  1:206519/85‑1 (MQ=255)

                                                                                                 cATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTAt                                                                   <  1:1257044/89‑1 (MQ=255)

                                                                                                 cATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTAt                                                                   >  2:1257044/1‑89 (MQ=255)

                                                                                                           tgctgcCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTa                                                                                                      >  1:1064018/1‑44 (MQ=255)

                                                                                                           tgctgcCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTa                                                                                                      <  2:1064018/44‑1 (MQ=255)

                                                                                                              tgcCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATg                                 <  1:1508748/110‑1 (MQ=255)

                                                                                                              tgcCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATg                                 >  2:1508748/1‑110 (MQ=255)

                                                                                                               gcCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCa   <  1:508050/139‑1 (MQ=255)

                                                                                                                ccACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCag  >  1:643593/1‑139 (MQ=255)

                                                                                                                     cTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGa           <  1:897978/125‑1 (MQ=255)

                                                                                                                     cTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGa           >  2:897978/1‑125 (MQ=255)

                                                                                                                       ggCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGgtgt                                                                            >  1:1105770/1‑58 (MQ=255)

                                                                                                                       ggCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGgtgt                                                                            <  2:1105770/58‑1 (MQ=255)

                                                                                                                          gcgGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATg                                                        >  2:923560/1‑75 (MQ=255)

                                                                                                                          gcgATGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGATCATTATGCAAATGGATg                                                        <  1:923560/75‑1 (MQ=255)

CGCCGAACTGCAGGCTGATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTGCACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAG  >  NC_000913/3434447‑3434697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A