Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F1 I1 R1 759 71.0 3673240 91.5% 3361014 104.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,434,577 G→A A122T (GCA→ACA)  fmt → 10‑formyltetrahydrofolate:L‑methionyl‑tRNA(fMet) N‑formyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,434,5770GA100.0% 183.1 / NA 57A122T (GCA→ACA) fmt10‑formyltetrahydrofolate:L‑methionyl‑tRNA(fMet) N‑formyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/32);  total (25/32)

GAACTGCAGGCTGATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTGCACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGG  >  NC_000913/3434451‑3434710
                                                                                                                              |                                                                                                                                      
gAACTGCAGGCTGATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAAc                                                                                                                            <  1:510826/139‑1 (MQ=255)
 aaCTGCAGGCCCATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAAca                                                                                                                           <  2:1199407/139‑2 (MQ=255)
     gCAGGCTGATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCT‑Ca                                                                                                                      >  2:1323849/1‑139 (MQ=255)
       aGGCTGATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACt                                                                                                                    <  1:894522/139‑1 (MQ=255)
                 tATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGt                                                                                                          <  2:477147/139‑1 (MQ=255)
                   tGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATcc                                                                                                                               <  2:1623188/117‑1 (MQ=255)
                   tGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATcc                                                                                                                               >  1:1623188/1‑117 (MQ=255)
                   tGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGa                                                                                                        >  2:1717354/1‑139 (MQ=255)
                    ggTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGAt                                                                                                       <  1:1251901/139‑1 (MQ=255)
                      tcgtcgtcgCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGc                                                                                                     <  2:1777324/139‑1 (MQ=255)
                        gtcgtcgCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAg                                                                                                   >  2:108479/1‑139 (MQ=255)
                          cgtcgCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCgg                                                                                                            >  2:62798/1‑128 (MQ=255)
                          cgtcgCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCgg                                                                                                            <  1:62798/128‑1 (MQ=255)
                                       ttAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGGTGTGACCa                                                                                    <  1:108479/139‑1 (MQ=255)
                                       ttAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGGTGTGACCa                                                                                    <  2:601249/139‑1 (MQ=255)
                                        tAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCa                                                                                                                              <  2:787703/97‑1 (MQ=255)
                                        tAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCa                                                                                                                              >  1:787703/1‑97 (MQ=255)
                                               gCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCaa                                                                            >  2:642866/1‑139 (MQ=255)
                                                      gCAGTGTTGGAGAGGCCGGGTTTTGGTTGAATCAACG‑TCAATGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGt                                                                    <  2:580188/139‑1 (MQ=255)
                                                          tGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCAT‑GTTCCCTTGTTCCACCCTTGCGCCGTTCTAAAACAAATCAACGCTCCACTATGGCCGGGTGTTCAAGAACCGGGTGTG‑CCAATATTGAAATGGaatgcggg                                                                >  1:580188/1‑132 (MQ=255)
                                                                   tGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGacac                                                        <  1:1323849/139‑1 (MQ=255)
                                                                    gCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTgg                                                                                            <  2:1832202/102‑1 (MQ=255)
                                                                    gCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTgg                                                                                            >  1:1832202/1‑102 (MQ=255)
                                                                    gCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACAcc                                                       >  1:333156/1‑139 (MQ=255)
                                                                      cgcgTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTg                                                                                                         >  2:473699/1‑87 (MQ=255)
                                                                      cgcgTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTg                                                                                                         <  1:473699/87‑1 (MQ=255)
                                                                          tCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGat                                                 <  1:1117465/139‑1 (MQ=255)
                                                                              ggCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGc                                             <  2:333156/139‑1 (MQ=255)
                                                                                     tCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATaa                                      >  1:1628543/1‑139 (MQ=255)
                                                                                          gTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGctct                                 <  2:1628543/139‑1 (MQ=255)
                                                                                             cATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAGCGCT‑CACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATg                                              >  1:1297167/1‑123 (MQ=255)
                                                                                             cATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTg                                                   >  2:688221/1‑118 (MQ=255)
                                                                                             cATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTg                                                   <  1:688221/118‑1 (MQ=255)
                                                                                             cATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATg                                              <  2:1297167/123‑1 (MQ=255)
                                                                                               tGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGat                                                 >  2:793420/1‑118 (MQ=255)
                                                                                               tGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGat                                                 <  1:793420/118‑1 (MQ=255)
                                                                                                     aCTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGGTGTGACCAt                                                                                   <  2:244844/78‑1 (MQ=255)
                                                                                                     aCTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGGTGTGACCAt                                                                                   >  1:244844/1‑78 (MQ=255)
                                                                                                     aCTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGAtt                      >  1:86381/1‑139 (MQ=255)
                                                                                                       tgctgcCACGCTGGCGCGGTGCTACACCAATCCAACGCt                                                                                                                         <  1:1067899/39‑1 (MQ=38)
                                                                                                       tgctgcCACGCTGGCGCGGTGCTACACCAATCCAACGCt                                                                                                                         >  2:1067899/1‑39 (MQ=38)
                                                                                                       tgctgcCACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGgtgt                                                                                         <  1:823279/70‑1 (MQ=255)
                                                                                                       tgctgcCACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGgtgt                                                                                         >  2:823279/1‑70 (MQ=255)
                                                                                                          tgcCACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGc                 >  2:146052/1‑139 (MQ=255)
                                                                                                            ccACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCa                                                                             >  1:1230716/1‑77 (MQ=255)
                                                                                                            ccACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCa                                                                             <  2:1230716/77‑1 (MQ=255)
                                                                                                            ccACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTg                  <  2:531837/136‑1 (MQ=255)
                                                                                                            ccACGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTg                  >  1:531837/1‑136 (MQ=255)
                                                                                                              aCGCTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCagaa             <  1:146052/139‑1 (MQ=255)
                                                                                                                 cTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGaca                                                         >  2:199849/1‑92 (MQ=255)
                                                                                                                 cTGGCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGaca                                                         <  1:199849/92‑1 (MQ=255)
                                                                                                                   ggCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCa                >  2:1559816/1‑131 (MQ=255)
                                                                                                                   ggCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCa                <  1:1559816/131‑1 (MQ=255)
                                                                                                                   ggCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATAc        >  2:1448874/1‑139 (MQ=255)
                                                                                                                   ggCGCGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATAc        <  1:1717354/139‑1 (MQ=255)
                                                                                                                    gcgcGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATAc        <  2:687155/138‑1 (MQ=255)
                                                                                                                    gcgcGGTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATAc        >  1:687155/1‑138 (MQ=255)
                                                                                                                         gTGCTACACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTgg  <  2:86381/139‑1 (MQ=255)
                                                                                                                              |                                                                                                                                      
GAACTGCAGGCTGATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTGCACCAATCCAACGCT‑CACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGG  >  NC_000913/3434451‑3434710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A