Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I1 R1	757	76.1	3598070	93.4%	3360597	108.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,434,577	G→A	A122T (GCA→ACA)	fmt →	10‑formyltetrahydrofolate:L‑methionyl‑tRNA(fMet) N‑formyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,434,577	0	G	A	100.0%	208.5 / NA	64	A122T (GCA→ACA)	fmt	10‑formyltetrahydrofolate:L‑methionyl‑tRNA(fMet) N‑formyltransferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (29/35); total (29/35)

TCGCCGAACTGCAGGCTGATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTGCACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATAC  >  NC_000913/3434446‑3434704

tCGCCGAACTGCAGGCTGATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAAt                                                                                                                          >  2:274164/1‑139 (MQ=255)

 cGCCGAACTGCAGGCTGATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGTTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATc                                                                                                                         >  1:556451/1‑139 (MQ=255)

                   tGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACACTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCg                                                                                                       >  1:1438326/1‑139 (MQ=255)

                      tATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGt                                                                                                    <  2:1581197/139‑1 (MQ=255)

                        tGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGa                                                                                                  >  2:1073036/1‑139 (MQ=255)

                          gtcgtcgtcGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATg                                                                                                >  1:386663/1‑139 (MQ=255)

                            cgtcgtcgCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCa                                                                                                                       <  1:26809/114‑1 (MQ=255)

                            cgtcgtcgCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCa                                                                                                                       >  2:26809/1‑114 (MQ=255)

                            cgtcgtcgCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCa                                                                                              >  2:1495860/1‑139 (MQ=255)

                             gtcgtcgCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAg                                                                                             >  1:618923/1‑139 (MQ=255)

                              tcgtcgCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGa                                                                                            <  1:1073036/139‑1 (MQ=255)

                                 tcgCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAAc                                                                                         <  2:618923/139‑1 (MQ=255)

                                    ccTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTgg                                                                                      <  1:1495860/139‑1 (MQ=255)

                                     cTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGgt                                                                                     <  2:386663/139‑1 (MQ=255)

                                       aTGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGgtgt                                                                                   <  2:1515949/139‑1 (MQ=255)

                                            ttAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCa                                                                              <  2:387477/139‑1 (MQ=255)

                                             tAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCAt                                                                             >  1:569814/1‑139 (MQ=255)

                                              aaTTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCGCGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCAtt                                                                            >  1:1019365/1‑139 (MQ=255)

                                              aaTTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCAtt                                                                            >  2:621231/1‑139 (MQ=255)

                                                ttCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTAt                                                                          <  2:1574448/139‑1 (MQ=255)

                                                ttCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTAt                                                                          <  1:995587/139‑1 (MQ=255)

                                                    gCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGc                                                                                               >  2:1767968/1‑114 (MQ=255)

                                                    gCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGc                                                                                               <  1:1767968/114‑1 (MQ=255)

                                                    gCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCaa                                                                      <  2:937412/139‑1 (MQ=255)

                                                         aaGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGa                                                                 <  2:1798728/139‑1 (MQ=255)

                                                           gCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCCAATGGATg                                                               <  2:1313174/139‑1 (MQ=255)

                                                            cAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGt                                                              <  1:1655579/139‑1 (MQ=255)

                                                                     agaTGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGa                                                     <  1:274164/139‑1 (MQ=255)

                                                                       aTGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACg                                                                                                                    <  2:1383898/74‑1 (MQ=255)

                                                                       aTGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACg                                                                                                                    >  1:1383898/1‑74 (MQ=255)

                                                                       aTGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGG‑GGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGacac                                                  <  2:1226276/139‑1 (MQ=255)

                                                                         gCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGgtgt                                                                                   >  2:373354/1‑105 (MQ=255)

                                                                         gCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGgtgt                                                                                   <  1:373354/105‑1 (MQ=255)

                                                                         gCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACAcc                                                 >  2:778558/1‑139 (MQ=255)

                                                                         gCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACAcc                                                 >  2:1599757/1‑139 (MQ=255)

                                                                           cgcgTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGgtgt                                                                                   <  2:189875/103‑1 (MQ=255)

                                                                           cgcgTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGgtgt                                                                                   >  1:189875/1‑103 (MQ=255)

                                                                                 ttGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGatat                                         <  1:1182939/139‑1 (MQ=255)

                                                                                 ttGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGatat                                         <  2:1019365/139‑1 (MQ=255)

                                                                                  tGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGa                                                     >  2:523425/1‑126 (MQ=255)

                                                                                  tGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGa                                                     <  1:523425/126‑1 (MQ=255)

                                                                                          tCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACg                                                                                                                    <  2:1723228/55‑1 (MQ=255)

                                                                                          tCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACg                                                                                                                    >  1:1723228/1‑55 (MQ=255)

                                                                                          tCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATg                                                               >  1:1793499/1‑108 (MQ=255)

                                                                                          tCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATg                                                               <  2:1793499/108‑1 (MQ=255)

                                                                                           cAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAg                               <  2:1774457/139‑1 (MQ=255)

                                                                                              cGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATg                                        >  2:160353/1‑127 (MQ=255)

                                                                                              cGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATg                                        <  1:160353/127‑1 (MQ=255)

                                                                                                  cATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGc                                                                                               <  2:93729/68‑1 (MQ=255)

                                                                                                  cATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGc                                                                                               >  1:93729/1‑68 (MQ=255)

                                                                                                  cATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGat                                           >  2:1593654/1‑120 (MQ=255)

                                                                                                  cATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGat                                           <  1:1593654/120‑1 (MQ=255)

                                                                                                  cATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCt                        >  2:773296/1‑139 (MQ=255)

                                                                                                         cACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATg                                        >  2:1738801/1‑116 (MQ=255)

                                                                                                         cACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATg                                        <  1:1738801/116‑1 (MQ=255)

                                                                                                             gctgcCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGgtgt                                                                                   >  1:1403091/1‑69 (MQ=255)

                                                                                                             gctgcCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGgtgt                                                                                   <  2:1403091/69‑1 (MQ=255)

                                                                                                                gcCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGgtgt                                                                                   <  2:342265/66‑1 (MQ=255)

                                                                                                                gcCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGgtgt                                                                                   >  1:342265/1‑66 (MQ=255)

                                                                                                                 ccccGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAAAAGCTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCAGATTACTGCag         <  2:1639563/136‑1 (MQ=255)

                                                                                                                   aCGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCagaa       <  1:773296/139‑1 (MQ=255)

                                                                                                                        ggCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCAt                                                                             <  1:1234473/64‑1 (MQ=255)

                                                                                                                        ggCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCAt                                                                             >  2:1234473/1‑64 (MQ=255)

                                                                                                                        ggCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATAc  >  2:676895/1‑139 (MQ=255)

TCGCCGAACTGCAGGCTGATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTGCACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATAC  >  NC_000913/3434446‑3434704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A