Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	770	51.4	2439994	93.8%	2288714	110.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,434,577	G→A	A122T (GCA→ACA)	fmt →	10‑formyltetrahydrofolate:L‑methionyl‑tRNA(fMet) N‑formyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,434,577	0	G	A	100.0%	105.3 / NA	33	A122T (GCA→ACA)	fmt	10‑formyltetrahydrofolate:L‑methionyl‑tRNA(fMet) N‑formyltransferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/17); total (16/17)

AACTGCAGGCTGATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTGCACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAG  >  NC_000913/3434452‑3434707

aaCTGCAGGCTGATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACg                                                                                                                       <  2:688927/139‑1 (MQ=255)

aaCTGCAGGCTGATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACg                                                                                                                       <  1:724518/139‑1 (MQ=255)

                 aTGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTg                                                                                                      <  2:574141/139‑1 (MQ=255)

                        tcgtcgCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGa                                                                                               >  1:365163/1‑139 (MQ=255)

                          gtcgCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGaaa                                                                                             <  1:614008/139‑1 (MQ=255)

                                 aTGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGgtgt                                                                                      <  1:1032169/139‑1 (MQ=255)

                                       tAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGgtgt                                                                                      <  2:757505/133‑1 (MQ=255)

                                       tAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGgtgt                                                                                      >  1:757505/1‑133 (MQ=255)

                                               ccGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCAt                                                                                >  2:852898/1‑131 (MQ=255)

                                               ccGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCAt                                                                                <  1:852898/131‑1 (MQ=255)

                                                         tGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCgg                                                              >  1:819924/1‑139 (MQ=255)

                                                                 aTGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGa                                                                                    >  1:668211/1‑109 (MQ=255)

                                                                 aTGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGa                                                                                    <  2:668211/109‑1 (MQ=255)

                                                                     cgcgTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACg                                                                                                                       >  1:504509/1‑70 (MQ=255)

                                                                     cgcgTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACg                                                                                                                       <  2:504509/70‑1 (MQ=255)

                                                                         tCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGat                                              >  2:916882/1‑139 (MQ=255)

                                                                                  tATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCtat                                     >  1:425197/1‑139 (MQ=255)

                                                                                    tCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCa                                                                                                 >  1:92004/1‑77 (MQ=255)

                                                                                    tCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCa                                                                                                 <  2:92004/77‑1 (MQ=255)

                                                                                            cATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATg                                                                  <  2:103106/100‑1 (MQ=255)

                                                                                            cATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATg                                                                  >  1:103106/1‑100 (MQ=255)

                                                                                              tGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGc                         <  2:425197/139‑1 (MQ=255)

                                                                                                   cACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGAt                    <  2:1106046/139‑1 (MQ=255)

                                                                                                   cACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGAt                    <  1:378668/139‑1 (MQ=255)

                                                                                                    aCTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGAtt                   >  1:1154047/1‑139 (MQ=255)

                                                                                                     ctgctgCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTa                  <  2:1154047/139‑1 (MQ=255)

                                                                                                        ctgcCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTg               >  1:676535/1‑139 (MQ=255)

                                                                                                          gcCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCa             <  2:819924/139‑1 (MQ=255)

                                                                                                                cTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGt                                                                                                       <  1:786777/43‑1 (MQ=255)

                                                                                                                cTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGt                                                                                                       >  2:786777/1‑43 (MQ=255)

                                                                                                                cTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGAt       >  2:279011/1‑139 (MQ=255)

                                                                                                                    cgcgGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCa   >  2:925636/1‑139 (MQ=255)

                                                                                                                     gcgGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAg  >  1:1069772/1‑139 (MQ=255)

AACTGCAGGCTGATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTGCACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAG  >  NC_000913/3434452‑3434707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A