Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F1 I2 R1 780 68.3 3343050 93.5% 3125751 102.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,466,313 A→G L138P (CTT→CCT)  bfr ← bacterioferritin, iron storage and detoxification protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,466,3130AG100.0% 226.3 / NA 63L138P (CTT→CCT) bfrbacterioferritin, iron storage and detoxification protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/29);  total (34/29)

CCTGGGGTATCTGGCCTGGATCTCTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAAGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATAACCAATTGCCTCACGCAAATTCTTCGCGCCATCCAGCTCA  >  NC_000913/3466190‑3466447
                                                                                                                           |                                                                                                                                      
ccTGGGGTATCTGGCCTGGATCTCTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTccagcc                                                                                                                         <  1:137625/139‑1 (MQ=255)
        aTCTGGCCTGGATCTCTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGata                                                                                                                 <  1:523558/139‑1 (MQ=255)
                    tctcTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAAACAGATTAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTtca                                                                                                     <  2:1117555/139‑1 (MQ=255)
                       ctTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTtcatca                                                                                                  >  1:1647141/1‑139 (MQ=255)
                             aaCCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGt                                                                                                                      <  2:1252599/113‑1 (MQ=255)
                             aaCCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGt                                                                                                                      >  1:1252599/1‑113 (MQ=255)
                              aCCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTccag                                                                                                                           >  2:871853/1‑107 (MQ=255)
                              aCCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTccag                                                                                                                           <  1:871853/107‑1 (MQ=255)
                                  ggAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGcc                                                                                                            <  2:599822/118‑1 (MQ=255)
                                  ggAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGcc                                                                                                            >  1:599822/1‑118 (MQ=255)
                                  ggAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTc                                                                                       >  2:193194/1‑139 (MQ=255)
                                  ggAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTc                                                                                       <  1:176415/139‑1 (MQ=255)
                                  ggAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTc                                                                                       >  1:1229882/1‑139 (MQ=255)
                                  ggAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTc                                                                                       >  2:1188389/1‑139 (MQ=255)
                                    aGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATg                                                                                                               <  1:1496050/113‑1 (MQ=255)
                                    aGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATg                                                                                                               >  2:1496050/1‑113 (MQ=255)
                                          ctccctccCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATg                                                                                                               <  2:590630/107‑1 (MQ=255)
                                          ctccctccCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATg                                                                                                               >  1:590630/1‑107 (MQ=255)
                                                cccGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTccag                                                                                                                           >  2:12600/1‑89 (MQ=255)
                                                cccGACACGGCGCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTccag                                                                                                                           <  1:12600/89‑1 (MQ=255)
                                                 ccGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTc                                                                                       <  2:1377853/124‑1 (MQ=255)
                                                 ccGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTc                                                                                       >  1:1377853/1‑124 (MQ=255)
                                                          cTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGc                                                                                               >  2:807908/1‑107 (MQ=255)
                                                          cTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGc                                                                                               <  1:807908/107‑1 (MQ=255)
                                                          cTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATcg                                                                          >  2:1058784/1‑128 (MQ=255)
                                                          cTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCGTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATcg                                                                          <  1:1058784/128‑1 (MQ=255)
                                                               ccTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCatat                                                                            <  2:106910/121‑1 (MQ=255)
                                                               ccTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCatat                                                                            >  1:106910/1‑121 (MQ=255)
                                                                    ttcGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTccag                                                                                                                           <  1:791734/69‑1 (MQ=255)
                                                                    ttcGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTccag                                                                                                                           >  2:791734/1‑69 (MQ=255)
                                                                    ttcGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGaa                                                       <  1:745056/137‑1 (MQ=255)
                                                                    ttcGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGaa                                                       >  2:745056/1‑137 (MQ=255)
                                                                                tgCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCg                                                                                                                    >  2:1531241/1‑64 (MQ=255)
                                                                                tgCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCg                                                                                                                    <  1:1531241/64‑1 (MQ=255)
                                                                                     gCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTccagccag                                                                                                                       >  2:1567222/1‑56 (MQ=255)
                                                                                     gCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTccagccag                                                                                                                       <  1:1567222/56‑1 (MQ=255)
                                                                                     gCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATAACCAAt                                    >  1:595226/1‑139 (MQ=255)
                                                                                       aGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATAACCAATTg                                  >  1:1563808/1‑139 (MQ=255)
                                                                                          tAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCAt                                           >  1:674760/1‑127 (MQ=255)
                                                                                          tAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCAt                                           <  2:674760/127‑1 (MQ=255)
                                                                                          tAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATAACCAATTGCCt                               >  2:1614984/1‑139 (MQ=255)
                                                                                          tAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATAACCAATTGCCt                               <  1:1614984/139‑1 (MQ=255)
                                                                                           aaTTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTccagcc                                                                                                                         >  1:643417/1‑48 (MQ=255)
                                                                                           aaTTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTccagcc                                                                                                                         <  2:643417/48‑1 (MQ=255)
                                                                                                     gCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGcc                                                                                                            <  2:1639822/51‑1 (MQ=255)
                                                                                                     gCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGcc                                                                                                            >  1:1639822/1‑51 (MQ=255)
                                                                                                       ccATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTc                                                                                       >  1:395305/1‑70 (MQ=255)
                                                                                                       ccATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTc                                                                                       <  2:395305/70‑1 (MQ=255)
                                                                                                       ccATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATAACCAATTGCCTCACGCAAAttctt                  >  2:233570/1‑139 (MQ=255)
                                                                                                          tcttctGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATAACCAATTGCCTCACGCAAATTCTTcgc               >  1:408279/1‑139 (MQ=255)
                                                                                                               tGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATAACCAATTGCCTCACGCAAATTCTTCGCGCCAt          >  2:646599/1‑139 (MQ=255)
                                                                                                                   tCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATAACCAATTGCCTCACGCAAATTCTTcgcg              >  1:1491351/1‑131 (MQ=255)
                                                                                                                   tCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATAACCAATTGCCTCACGCAAATTCTTcgcg              <  2:1491351/131‑1 (MQ=255)
                                                                                                                      gATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATcg                                                                          <  2:308335/68‑1 (MQ=255)
                                                                                                                      gATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATcg                                                                          >  1:308335/1‑68 (MQ=255)
                                                                                                                      gATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATAACCAATTGCCTCACGCAAATTCTTCGCGCCATCCAGc     >  2:337218/1‑137 (MQ=255)
                                                                                                                      gATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATAACCAATTGCCTCACGCAAATTCTTCGCGCCATCCAGc     <  1:337218/137‑1 (MQ=255)
                                                                                                                      gATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATAACCAATTGCCTCACGCAAATTCTTCGCGCCATCCAGCTc   >  1:443305/1‑139 (MQ=255)
                                                                                                                       aTCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGTCATAACCAATTGCCTCACGCAAATTCTTCGCGCCATCCAGCTCa  <  1:193194/139‑1 (MQ=255)
                                                                                                                       aTCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATAACCAATTGCCTCACGCAAATTCTTcgcg              <  1:1593559/127‑1 (MQ=255)
                                                                                                                       aTCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATAACCAATTGCCTCACGCAAATTCTTcgcg              >  2:1593559/1‑127 (MQ=255)
                                                                                                                       aTCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATAACAAATTGCCTCACGCaaa                       <  2:569841/118‑1 (MQ=255)
                                                                                                                       aTCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATAACAAATTGCCTCACGCaaa                       >  1:569841/1‑118 (MQ=255)
                                                                                                                           |                                                                                                                                      
CCTGGGGTATCTGGCCTGGATCTCTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAAGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATAACCAATTGCCTCACGCAAATTCTTCGCGCCATCCAGCTCA  >  NC_000913/3466190‑3466447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A