Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F1 I1 R1	765	39.6	1867486	94.4%	1762906	108.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,466,313	A→G	L138P (CTT→CCT)	bfr ←	bacterioferritin, iron storage and detoxification protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,466,313	0	A	G	100.0%	156.0 / NA	44	L138P (CTT→CCT)	bfr	bacterioferritin, iron storage and detoxification protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/20); total (24/20)

CATGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAAGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATAACCAATTGCCTCACGCAAATTCTTCGCGCCATCCAGCTC  >  NC_000913/3466181‑3466446

caTGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCg                                                                                                                                 >  1:506176/1‑139 (MQ=255)

          cTGGGGTATCTGGCCTGGATCTCTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTccagcca                                                                                                                       <  1:374514/139‑1 (MQ=255)

          cTGGGGTATCTGGCCTGGATCTCTTATTAACCGGGAGGGATCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTccagcca                                                                                                                       >  2:410930/1‑139 (MQ=255)

             gggTATCTGGCCTGGATCTCTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTc                                                                                                                    >  1:730818/1‑139 (MQ=255)

                 aTCTGGCCTGGATCTCTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTc                                                                                                                                   <  2:429035/120‑1 (MQ=255)

                 aTCTGGCCTGGATCTCTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTc                                                                                                                                   >  1:429035/1‑120 (MQ=255)

                       ccTGGATCTCTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCct                                                                                                          >  1:896103/1‑139 (MQ=255)

                         tGGATCTCTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGCATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGt                                                                                                                     <  1:580991/126‑1 (MQ=255)

                         aggATCTCTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGt                                                                                                                     >  2:580991/2‑126 (MQ=255)

                             tctcTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTtca                                                                                                    <  2:834459/139‑1 (MQ=255)

                                ctTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATg                                                                                                              >  1:768021/1‑126 (MQ=255)

                                ctTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATg                                                                                                              <  2:768021/126‑1 (MQ=255)

                                ctTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTtcatca                                                                                                 >  2:697333/1‑139 (MQ=255)

                                         cGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAAtt                                                                                        >  1:355219/1‑139 (MQ=255)

                                           ggAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGTCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTc                                                                                      >  2:542885/1‑139 (MQ=255)

                                           ggAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTc                                                                                      >  2:580046/1‑139 (MQ=255)

                                           ggAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTc                                                                                      >  2:146499/1‑139 (MQ=255)

                                             aGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATTTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTa                                                                                    <  1:580046/139‑1 (MQ=255)

                                                           cGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCgg                                                                      >  2:926388/1‑139 (MQ=255)

                                                            gACACGGCTCAACCTTCTTCGCGGATCTTTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGc                                                                     <  2:730818/139‑1 (MQ=255)

                                                                cGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGAc                                                                 >  1:532574/1‑139 (MQ=255)

                                                                  gCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGt                                                               >  1:335234/1‑139 (MQ=255)

                                                                   cTCAACCTTCTTCGCGGATCTGTGCTTGCTGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTccag                                                                                                                          >  2:537357/1‑79 (MQ=255)

                                                                   cTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTccag                                                                                                                          <  1:537357/79‑1 (MQ=255)

                                                                    tCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGt                                                                                                                     <  2:212644/83‑1 (MQ=255)

                                                                    tCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGt                                                                                                                     >  1:212644/1‑83 (MQ=255)

                                                                         cttcttCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATg                                                        <  2:557796/139‑1 (MQ=255)

                                                                           tcttcGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGaa                                                      <  1:146499/139‑1 (MQ=255)

                                                                               cgcgGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCt                                                  <  2:355219/139‑1 (MQ=255)

                                                                                gcgGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTa                                                 >  2:337631/1‑139 (MQ=255)

                                                                                gcgGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTa                                                 <  2:335234/139‑1 (MQ=255)

                                                                                       tgtgCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCAt                                          <  1:337631/139‑1 (MQ=255)

                                                                                                   tAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATAACCAATTGCCt                              <  1:926388/139‑1 (MQ=255)

                                                                                                                   tcttctGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATcgcg                                                                       >  2:103319/1‑82 (MQ=255)

                                                                                                                   tcttctGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATcgcg                                                                       <  1:103319/82‑1 (MQ=255)

                                                                                                                               gATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATAACCAATTGCCTCACACAAATTCTTcgcg             <  2:400483/128‑1 (MQ=255)

                                                                                                                               gATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATAACCAATTGCCTCACACAAATTCTTcgcg             >  1:400483/1‑128 (MQ=255)

                                                                                                                               gATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGCAATCATGAACGCTATCGGCATAACCAATTGCCTCACGCAAATTCTTCGCGCCATCCAGCTc  >  1:817480/1‑139 (MQ=255)

                                                                                                                                aTCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTa                                                 <  2:502688/91‑1 (MQ=255)

                                                                                                                                aTCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTa                                                 >  1:502688/1‑91 (MQ=255)

                                                                                                                                aTCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTAt                                                >  1:761863/1‑92 (MQ=255)

                                                                                                                                aTCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTAt                                                <  2:761863/92‑1 (MQ=255)

                                                                                                                                aTCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATAACCAATTGCCTCACGCAAATTCTTcgcg             >  1:580072/1‑127 (MQ=255)

                                                                                                                                aTCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATAACCAATTGCCTCACGCAAATTCTTcgcc             <  2:580072/127‑2 (MQ=255)

CATGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAAGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATAACCAATTGCCTCACGCAAATTCTTCGCGCCATCCAGCTC  >  NC_000913/3466181‑3466446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A