Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I2 R1	772	77.0	3769570	91.6%	3452926	105.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,162,308	G→A	S121L (TCG→TTG)	ftsP ←	septal ring component that protects the divisome from stress, multicopy suppressor of ftsI(Ts)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,162,308	0	G	A	100.0%	179.6 / NA	56	S121L (TCG→TTG)	ftsP	septal ring component that protects the divisome from stress, multicopy suppressor of ftsI(Ts)
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (30/26); total (30/26)

CATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGATAGT  >  NC_000913/3162174‑3162438

cATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAAcat                                                                                                                                >  2:88100/1‑139 (MQ=255)

            gCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCgg                                                                                                                    >  2:1111421/1‑139 (MQ=255)

             tcacaTTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGa                                                                                                                   >  2:127087/2‑139 (MQ=255)

                         aaGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGc                                                                                                       <  2:209349/139‑1 (MQ=255)

                             ccGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGc                                                                                                   <  2:936918/139‑1 (MQ=255)

                                 tataGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTgg                                                                                               <  2:513217/139‑1 (MQ=255)

                                 tataGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTgg                                                                                               <  2:383170/139‑1 (MQ=255)

                                          gctgAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGt                                                                                              <  1:1185182/131‑1 (MQ=255)

                                          gctgAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGt                                                                                              >  2:1185182/1‑131 (MQ=255)

                                          gctgAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAg                                                                                      >  2:1211231/1‑139 (MQ=255)

                                                     cGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGccc                                                                                                             <  2:590665/105‑1 (MQ=255)

                                                     cGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGccc                                                                                                             >  1:590665/1‑105 (MQ=255)

                                                          gggAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATAGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTTAtt                                                                      <  1:1211231/139‑1 (MQ=255)

                                                            gAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTga                                                                    <  2:311929/139‑1 (MQ=255)

                                                               tATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGAc                                                                 >  1:1449878/1‑139 (MQ=255)

                                                                  tGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGc                                                                                     <  2:509509/116‑1 (MQ=255)

                                                                  tGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGc                                                                                     >  1:509509/1‑116 (MQ=255)

                                                                  tGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTgaga                                                                  >  1:1477437/1‑135 (MQ=255)

                                                                  tGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTgaga                                                                  <  2:1477437/135‑1 (MQ=255)

                                                                  tGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACAtt                                                              >  2:1626087/1‑139 (MQ=255)

                                                                       tGATACCACAGAGCAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTg                                                         >  1:823391/1‑139 (MQ=255)

                                                                         aTACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTc                                                       >  2:808532/1‑139 (MQ=255)

                                                                               caGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCgg                                                 <  2:782155/139‑1 (MQ=255)

                                                                               caGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCgg                                                 <  2:1411420/139‑1 (MQ=255)

                                                                                agtgTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCTTGGTACCTGTTGCCCGGCCACCGTCATTGAGACATTTTCTGTCAggtggt                                                <  1:469290/136‑1 (MQ=255)

                                                                                  agTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCCGCTGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGt                                                >  1:321224/1‑137 (MQ=255)

                                                                                  agTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGt                                                <  2:321224/137‑1 (MQ=255)

                                                                                  agTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTg                                              >  1:743779/1‑139 (MQ=255)

                                                                                    tAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAg                                                                                                        <  1:1410799/79‑1 (MQ=255)

                                                                                    tAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAg                                                                                                        >  2:1410799/1‑79 (MQ=255)

                                                                                    tAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCt                                            <  1:127087/139‑1 (MQ=255)

                                                                                    tAGCTGCGTTCTCGCGACTAGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCt                                            >  1:1690995/1‑139 (MQ=255)

                                                                                       cTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGa                                                                                                                   >  2:182850/1‑65 (MQ=255)

                                                                                       cTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGa                                                                                                                   <  1:182850/65‑1 (MQ=255)

                                                                                       cTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTa                                         >  2:815615/1‑139 (MQ=255)

                                                                                            ttCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCAtt                                                                      >  1:741181/1‑105 (MQ=255)

                                                                                            ttCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCAtt                                                                      <  2:741181/105‑1 (MQ=255)

                                                                                               tGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCt                                 >  1:375729/1‑139 (MQ=255)

                                                                                                      tGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACAtc                          <  1:815615/139‑1 (MQ=255)

                                                                                                         gccgTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAg                                                                                                        >  1:288174/4‑58 (MQ=255)

                                                                                                         gCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAg                                                                                                        <  2:288174/58‑1 (MQ=255)

                                                                                                           aGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGc                     <  1:1652415/139‑1 (MQ=255)

                                                                                                             tACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGccc                   >  2:374749/1‑139 (MQ=255)

                                                                                                                  gTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGAc                                                                                                                  >  2:1361537/1‑39 (MQ=255)

                                                                                                                  gTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGAc                                                                                                                  <  1:1361537/39‑1 (MQ=255)

                                                                                                                  gTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGc                                                                                                   >  1:727970/1‑54 (MQ=255)

                                                                                                                  gTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGc                                                                                                   <  2:727970/54‑1 (MQ=255)

                                                                                                                  gTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCa              >  2:1578289/1‑139 (MQ=255)

                                                                                                                    gCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGa            <  2:823391/139‑1 (MQ=255)

                                                                                                                     cccAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAACTTAACATCGTCGCCCTTCCAGAc           >  2:1048762/1‑139 (MQ=255)

                                                                                                                       cAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCAcc                                                                            >  2:1170213/1‑72 (MQ=255)

                                                                                                                       cAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCAcc                                                                            <  1:1170213/72‑1 (MQ=255)

                                                                                                                       cAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGAcgc         >  1:1140158/1‑139 (MQ=255)

                                                                                                                          tCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGa      >  1:790753/1‑139 (MQ=255)

                                                                                                                              cGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGATAGt  <  1:1111421/139‑1 (MQ=255)

                                                                                                                              cGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGATAGt  >  1:1741141/1‑139 (MQ=255)

CATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGATAGT  >  NC_000913/3162174‑3162438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A