Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I2 R1	766	55.6	2631458	94.7%	2491990	109.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,162,308	G→A	S121L (TCG→TTG)	ftsP ←	septal ring component that protects the divisome from stress, multicopy suppressor of ftsI(Ts)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,162,308	0	G	A	100.0%	170.8 / NA	53	S121L (TCG→TTG)	ftsP	septal ring component that protects the divisome from stress, multicopy suppressor of ftsI(Ts)
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/32); total (21/32)

TTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGATAGTCG  >  NC_000913/3162172‑3162440

ttCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAAc                                                                                                                                    <  2:1059212/139‑1 (MQ=255)

     cTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAAcatcat                                                                                                                               <  2:495872/139‑1 (MQ=255)

                              gCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCggg                                                                                                      <  2:56680/139‑1 (MQ=255)

                               ccGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGc                                                                                                     <  2:395911/139‑1 (MQ=255)

                                  ttATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTg                                                                                                  <  1:548311/139‑1 (MQ=255)

                                   tataGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCGTCAGCGGGCCTgg                                                                                                 <  2:59246/139‑1 (MQ=255)

                                   tataGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTgg                                                                                                 <  2:202407/139‑1 (MQ=255)

                                          ctgctgAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGt                                                                                          >  1:1166372/1‑139 (MQ=255)

                                               gAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAAcatcat                                                                                                                               <  2:96121/97‑1 (MQ=255)

                                               gAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAAcatcat                                                                                                                               >  1:96121/1‑97 (MQ=255)

                                                     tGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGAc                                                                                                                    <  1:645840/102‑1 (MQ=255)

                                                     tGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGAc                                                                                                                    >  2:645840/1‑102 (MQ=255)

                                                                    tGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAAcatcat                                                                                                                               <  2:763390/76‑1 (MQ=255)

                                                                    tGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAAcatcat                                                                                                                               >  1:763390/1‑76 (MQ=255)

                                                                    tGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACAtt                                                                >  2:1267877/1‑139 (MQ=255)

                                                                    tGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACAtt                                                                >  2:936545/1‑139 (MQ=255)

                                                                      gCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACAtttt                                                              <  2:922400/139‑1 (MQ=255)

                                                                            tACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCa                                                        <  2:423631/139‑1 (MQ=255)

                                                                               cacaGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGc                                                     <  1:139510/139‑1 (MQ=255)

                                                                                 caGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCgg                                                   <  2:736801/139‑1 (MQ=255)

                                                                                 caGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCgg                                                   <  2:1114878/139‑1 (MQ=255)

                                                                                  agagTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGt                                                  <  1:886257/139‑1 (MQ=255)

                                                                                    agTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCg                                                                                                                       <  2:188463/68‑1 (MQ=255)

                                                                                    agTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCg                                                                                                                       >  1:188463/1‑68 (MQ=255)

                                                                                    agTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTg                                                <  2:1159909/139‑1 (MQ=255)

                                                                                      tAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAAcat                                                                                                                                  <  2:394270/55‑1 (MQ=255)

                                                                                      tAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAAcat                                                                                                                                  >  1:394270/1‑55 (MQ=255)

                                                                                      tAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGt                                                          >  1:366010/1‑127 (MQ=255)

                                                                                      tAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGt                                                          <  2:366010/127‑1 (MQ=255)

                                                                                      tAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCt                                              >  2:194191/1‑139 (MQ=255)

                                                                                      tAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCt                                              >  2:826396/1‑139 (MQ=255)

                                                                                        gCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCg                                                                                                        >  1:354041/1‑79 (MQ=255)

                                                                                        gCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCg                                                                                                        <  2:354041/79‑1 (MQ=255)

                                                                                            cGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAAt                                        <  1:826396/139‑1 (MQ=255)

                                                                                             gTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATa                                       <  2:703553/139‑1 (MQ=255)

                                                                                              ttCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATaa                                      >  1:555357/1‑139 (MQ=255)

                                                                                                       aTGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACAt                             <  2:1166372/139‑1 (MQ=255)

                                                                                                        tGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGATCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACAtc                            >  1:1061850/1‑139 (MQ=255)

                                                                                                          ggCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACAtcgt                          <  2:1282058/139‑1 (MQ=255)

                                                                                                             aGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTg                                                                       <  2:518973/91‑1 (MQ=255)

                                                                                                             aGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTg                                                                       >  1:518973/1‑91 (MQ=255)

                                                                                                                   ggTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCAcc                                                                              >  2:1131894/1‑78 (MQ=255)

                                                                                                                   ggTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCAcc                                                                              <  1:1131894/78‑1 (MQ=255)

                                                                                                                    gTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCa                >  1:932704/1‑139 (MQ=255)

                                                                                                                     tGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAg               <  1:610886/139‑1 (MQ=255)

                                                                                                                      gCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGa              <  1:139595/139‑1 (MQ=255)

                                                                                                                         cAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTgg                                                                                                 >  2:105803/1‑53 (MQ=255)

                                                                                                                         cAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTgg                                                                                                 <  1:105803/53‑1 (MQ=255)

                                                                                                                         cAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGAcgc           >  1:238473/1‑139 (MQ=255)

                                                                                                                           gTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCgg         >  1:1149399/1‑139 (MQ=255)

                                                                                                                                  tttGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGATAGTCg  <  2:432220/139‑1 (MQ=255)

                                                                                                                                     ggCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTgg                                                                                                 >  2:1417/1‑41 (MQ=255)

                                                                                                                                     ggCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTgg                                                                                                 <  1:1417/41‑1 (MQ=255)

TTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGATAGTCG  >  NC_000913/3162172‑3162440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A