Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F1 I1 R1 781 46.9 2231650 93.5% 2086592 109.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,162,308 G→A S121L (TCG→TTG)  ftsP ← septal ring component that protects the divisome from stress, multicopy suppressor of ftsI(Ts)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,162,3080GA100.0% 178.5 / NA 56S121L (TCG→TTG) ftsPseptal ring component that protects the divisome from stress, multicopy suppressor of ftsI(Ts)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/26);  total (30/26)

TTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGATAGT  >  NC_000913/3162172‑3162438
                                                                                                                                        |                                                                                                                                  
ttCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAAc                                                                                                                                  <  2:451493/139‑1 (MQ=255)
  cATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAAcat                                                                                                                                >  2:135627/1‑139 (MQ=255)
     cTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAAcatcat                                                                                                                             <  1:135627/139‑1 (MQ=255)
          accaGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTg                                                                                                                        >  1:830068/1‑139 (MQ=255)
          accaGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTg                                                                                                                        >  1:755633/1‑139 (MQ=255)
             aGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCg                                                                                                                     <  2:567760/139‑1 (MQ=255)
               ccACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTTTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGa                                                                                                                   >  2:331519/1‑139 (MQ=255)
                cacaTTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCATCATCATCCGTGCCGGAc                                                                                                                  <  1:410311/139‑1 (MQ=255)
                              gCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTTTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCggg                                                                                                    <  1:331519/139‑1 (MQ=255)
                               ccGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAAc                                                                                                                                  <  2:652104/108‑1 (MQ=255)
                               ccGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGc                                                                                                   <  1:349241/139‑1 (MQ=255)
                                    ataGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGt                                                                                              >  1:168821/1‑139 (MQ=255)
                                        aCCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCt                                                                                          >  1:758966/1‑139 (MQ=255)
                                             ctgAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGt                                                                                              >  1:910328/1‑130 (MQ=255)
                                             ctgAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGt                                                                                              <  2:910328/130‑1 (MQ=255)
                                             ctgAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCATGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGc                                                                                     >  1:289051/1‑139 (MQ=255)
                                               gAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGccc                                                                                   >  1:968636/1‑139 (MQ=255)
                                                     tGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCAc                                                                             >  2:580465/1‑139 (MQ=255)
                                                      gCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCAcc                                                                            <  2:496653/139‑1 (MQ=255)
                                                       cGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCg                                                                           >  1:756258/1‑139 (MQ=255)
                                                            gggAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCAtt                                                                      >  1:339165/1‑139 (MQ=255)
                                                                                 caGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCgg                                                 <  2:968636/139‑1 (MQ=255)
                                                                                  agagTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGt                                                <  2:168821/139‑1 (MQ=255)
                                                                                  agagTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCATGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGt                                                <  2:289051/139‑1 (MQ=255)
                                                                                    agTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGAc                                                                                                                  >  2:1021303/1‑71 (MQ=255)
                                                                                    agTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGAc                                                                                                                  <  1:1021303/71‑1 (MQ=255)
                                                                                     gTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGc                                             >  2:1090336/1‑139 (MQ=255)
                                                                                     gTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGc                                             <  2:758966/139‑1 (MQ=255)
                                                                                         cTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGa                                                                                                                   <  2:1038450/65‑1 (MQ=255)
                                                                                         cTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGa                                                                                                                   >  1:1038450/1‑65 (MQ=255)
                                                                                         cTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTa                                         >  1:532799/1‑139 (MQ=255)
                                                                                          tGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTaa                                        <  2:756258/139‑1 (MQ=255)
                                                                                              ttCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTgg                                                                                               <  2:1016006/80‑1 (MQ=255)
                                                                                              ttCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTgg                                                                                               >  1:1016006/1‑80 (MQ=255)
                                                                                              ttCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATaa                                    >  1:879146/1‑139 (MQ=255)
                                                                                                   gCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTa                               >  2:146033/1‑139 (MQ=255)
                                                                                                             aGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGc                     <  2:830068/139‑1 (MQ=255)
                                                                                                              gTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGcc                    >  2:74016/1‑139 (MQ=255)
                                                                                                               tACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGc                                                                                     <  1:963492/73‑1 (MQ=255)
                                                                                                               tACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGc                                                                                     >  2:963492/1‑73 (MQ=255)
                                                                                                                aCGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCt                  <  2:339165/139‑1 (MQ=255)
                                                                                                                  gggTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTc                <  2:755633/139‑1 (MQ=255)
                                                                                                                    gTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAg                                                                                                        >  2:295574/1‑49 (MQ=255)
                                                                                                                    gTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAg                                                                                                        <  1:295574/49‑1 (MQ=255)
                                                                                                                    gTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGc                                             <  2:909096/108‑1 (MQ=255)
                                                                                                                    gTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGc                                             >  1:909096/1‑108 (MQ=255)
                                                                                                                    gTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTAGCCCTTCCa              >  1:712134/1‑139 (MQ=255)
                                                                                                                      gCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCt                                            <  2:869974/107‑1 (MQ=255)
                                                                                                                      gCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCt                                            >  1:869974/1‑107 (MQ=255)
                                                                                                                         cAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTaa                                        >  1:46927/1‑108 (MQ=255)
                                                                                                                         cAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTaa                                        <  2:46927/108‑1 (MQ=255)
                                                                                                                            tCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGa      >  1:362906/1‑139 (MQ=255)
                                                                                                                             cAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGAt     >  2:294602/1‑139 (MQ=255)
                                                                                                                              aGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGc                                             <  1:40736/98‑1 (MQ=255)
                                                                                                                              aGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGc                                             >  2:40736/1‑98 (MQ=255)
                                                                                                                                cGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGATAGt  >  1:480121/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                  
TTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGATAGT  >  NC_000913/3162172‑3162438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A