Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F1 I2 R1
|
772 |
77.0 |
3769570 |
91.6% |
3452926 |
105.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,296,984 |
G→A |
*192* (TAG→TAA) |
yraP → |
outer membrane lipoprotein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,296,984 | 0 | G | A | 100.0%
| 130.3
/ NA
| 41 | *192* (TAG→TAA) | yraP | outer membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/22); total (19/22) |
AGTGACCACCGAAAACGGTGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAGCAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAA > NC_000913/3296852‑3297106
|
aGTGACCACCGAAAACGGTGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGccc < 1:1169854/139‑1 (MQ=255)
aGTGACCACCGAAAACGGTGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGccc < 2:889781/139‑1 (MQ=255)
accaccGAAAACGGTGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCTAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTg > 1:1165560/1‑139 (MQ=255)
accaccGAAAACGGTGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTg > 1:1023813/1‑139 (MQ=255)
gAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGa > 2:1127559/1‑139 (MQ=255)
aaGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATaaca < 1:166937/115‑1 (MQ=255)
ttCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATAGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTg > 1:786755/1‑139 (MQ=255)
gATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGc < 2:1425781/135‑1 (MQ=255)
gATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGc > 1:1425781/1‑135 (MQ=255)
aTGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGt < 1:58559/139‑1 (MQ=255)
ggggCTGGTAACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCt > 2:26154/1‑139 (MQ=255)
cTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATc < 2:627767/139‑1 (MQ=255)
tGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATaaca < 2:459016/94‑1 (MQ=255)
tGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc > 1:1109838/1‑139 (MQ=255)
gAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACa > 1:1417777/1‑139 (MQ=255)
aaGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGc < 1:1654740/112‑1 (MQ=255)
aaGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGc > 2:1654740/1‑112 (MQ=255)
gAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGt < 2:997561/87‑1 (MQ=255)
gAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGt > 1:997561/1‑87 (MQ=255)
cggcAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCg > 1:1796644/1‑133 (MQ=255)
cggcAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCg < 2:1796644/133‑1 (MQ=255)
ggcAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGccc > 1:205063/1‑73 (MQ=255)
ggcAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGccc < 2:205063/73‑1 (MQ=255)
atatTGCTAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATaa < 2:1165560/139‑1 (MQ=255)
gCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc > 2:555491/1‑101 (MQ=255)
gCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc < 1:555491/101‑1 (MQ=255)
ccGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCAACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTAcg > 1:275309/1‑139 (MQ=255)
ccGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGACTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTAcg > 1:280076/1‑139 (MQ=255)
ggTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTAcgccg < 2:1023813/139‑1 (MQ=255)
ggTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTAcgccg < 1:26154/139‑1 (MQ=255)
aGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATc < 1:1025818/139‑1 (MQ=255)
gcggcgTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATaaca > 1:912140/1‑46 (MQ=255)
gcggcgTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATaaca < 2:912140/46‑1 (MQ=255)
ggcgTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGa < 2:1109838/139‑1 (MQ=255)
cgTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACa < 1:1197223/139‑1 (MQ=255)
gTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCt < 2:280076/139‑1 (MQ=255)
gTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCt > 1:1327423/1‑139 (MQ=255)
aCTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCaa < 2:786755/139‑1 (MQ=255)
cGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAg < 1:7685/68‑1 (MQ=255)
cGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAg > 2:7685/1‑68 (MQ=255)
gtttacgtttaTTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc < 1:94547/67‑1 (MQ=255)
gtttacgtttaTTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc > 2:94547/1‑67 (MQ=255)
ttacgtttaTTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGaacaa < 1:1127559/139‑1 (MQ=255)
ttaAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTAt < 2:1351708/50‑1 (MQ=255)
ttaAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTAt > 1:1351708/1‑50 (MQ=255)
|
AGTGACCACCGAAAACGGTGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAGCAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAA > NC_000913/3296852‑3297106
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
