Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
748 |
32.0 |
1685426 |
92.6% |
1560704 |
103.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,296,984 |
G→A |
*192* (TAG→TAA) |
yraP → |
outer membrane lipoprotein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,296,984 | 0 | G | A | 100.0%
| 50.5
/ NA
| 17 | *192* (TAG→TAA) | yraP | outer membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (8/9); total (8/9) |
GTGACCACCGAAAACGGTGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAGCAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGAT > NC_000913/3296853‑3297059
|
gTGACCACCGAAAACGGTGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGcccc > 1:348884/1‑139 (MQ=255)
caccGAAAACGGTGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTa > 2:113252/1‑139 (MQ=255)
gAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGa > 2:739825/1‑139 (MQ=255)
aGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACg < 1:223821/139‑1 (MQ=255)
gCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTAt > 1:623770/1‑139 (MQ=255)
gCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTAt < 2:623770/139‑1 (MQ=255)
cTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATc < 2:340272/139‑1 (MQ=255)
aaGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCg < 2:348884/139‑1 (MQ=255)
aGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGAt < 1:739825/139‑1 (MQ=255)
gTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGccc < 1:765062/54‑1 (MQ=255)
gTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGccc > 2:765062/1‑54 (MQ=255)
cgtttacgtttaTTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGt > 1:801796/1‑90 (MQ=255)
cgtttacgtttaTTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGt < 2:801796/90‑1 (MQ=255)
tttacgtttaTTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGt > 1:377900/1‑55 (MQ=255)
tttacgtttaTTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGt < 2:377900/55‑1 (MQ=255)
tttacgtttaTTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAgg < 1:470179/64‑1 (MQ=255)
tttacgtttaTTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAgg > 2:470179/1‑64 (MQ=255)
|
GTGACCACCGAAAACGGTGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAGCAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGAT > NC_000913/3296853‑3297059
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
