Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I1 R1	760	58.2	3022526	90.8%	2744453	102.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,296,984	G→A	192 (TAG→TAA)	yraP →	outer membrane lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,296,984	0	G	A	100.0%	88.1 / NA	28	192 (TAG→TAA)	yraP	outer membrane lipoprotein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (15/13); total (15/13)

TGAAAGTGACCACCGAAAACGGTGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAGCAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACA  >  NC_000913/3296848‑3297108

tGAAAGTGACCACCGAAAACGGTGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATaaca                                                                                                                            <  1:28303/139‑1 (MQ=255)

        accaccGAAAACGGTGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTg                                                                                                                    >  2:223420/1‑139 (MQ=255)

                          gtgtTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGc                                                                                                  <  1:223420/139‑1 (MQ=255)

                                  gATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCcgcg                                                                                          <  2:648913/139‑1 (MQ=255)

                                       ggCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATaaca                                                                                                                            <  1:227095/100‑1 (MQ=255)

                                       ggCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTa                                                                                     >  1:234474/1‑139 (MQ=255)

                                       ggCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTa                                                                                     >  1:481164/1‑139 (MQ=255)

                                            gTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATaaca                                                                                                                            >  2:414991/1‑95 (MQ=255)

                                            gTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATaaca                                                                                                                            <  1:414991/95‑1 (MQ=255)

                                                cTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTa                                                                            <  2:234474/139‑1 (MQ=255)

                                                                aGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGt                                                            <  2:481164/139‑1 (MQ=255)

                                                                        cAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGa                                                    <  2:1305785/139‑1 (MQ=255)

                                                                           atatTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGc                                                                                              <  2:144657/94‑1 (MQ=255)

                                                                           atatTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGc                                                                                              >  1:144657/1‑94 (MQ=255)

                                                                                   aGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCg                                                             <  1:735462/119‑1 (MQ=255)

                                                                                   aGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCg                                                             >  2:735462/1‑119 (MQ=255)

                                                                                    gCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTAc                                        >  2:705219/1‑139 (MQ=255)

                                                                                    gCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTAc                                        >  2:969704/1‑139 (MQ=255)

                                                                                                   tGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGcccc                                                                                                                       <  1:939314/45‑1 (MQ=255)

                                                                                                   tGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGcccc                                                                                                                       >  2:939314/1‑45 (MQ=255)

                                                                                                   tGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCg                                                             >  2:1049037/1‑103 (MQ=255)

                                                                                                   tGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCg                                                             <  1:1049037/103‑1 (MQ=255)

                                                                                                         gggTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTcc                   <  1:969704/139‑1 (MQ=255)

                                                                                                                     cgtttacgtttaTTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGaa       >  1:700117/1‑139 (MQ=255)

                                                                                                                       tttacgtttaTTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTGCGCCGCATCCGACATGTTTTCCCTCAAATCAGaaca     >  2:163480/1‑139 (MQ=255)

                                                                                                                          acgtttaTTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTAcgc                                      <  1:1070321/103‑1 (MQ=255)

                                                                                                                          acgtttaTTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTAGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTAcgc                                      >  2:1070321/1‑103 (MQ=255)

                                                                                                                          acgtttaTTAAATAACAGCCCCTTGTAATGCCTGATACGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAacaaca  >  1:559871/1‑139 (MQ=255)

                                                                                                                               tattaAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCcgcg                                                                                          >  2:1473301/1‑46 (MQ=255)

                                                                                                                               tattaAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCcgcg                                                                                          <  1:1473301/46‑1 (MQ=255)

                                                                                                                               tattaAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCAt                                 >  2:1436178/1‑103 (MQ=255)

                                                                                                                               tattaAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCAt                                 <  1:1436178/103‑1 (MQ=255)

TGAAAGTGACCACCGAAAACGGTGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAGCAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACA  >  NC_000913/3296848‑3297108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A