Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F1 I1 R1
|
776 |
60.0 |
3074904 |
90.1% |
2770488 |
104.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,980,957 |
(G)6→7 |
coding (71/1386 nt) |
yifK → |
putative APC family amino acid transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,980,951 | 1 | . | G | 100.0%
| 209.6
/ NA
| 59 | L22R (CTG→CGG) | yifK | putative APC family amino acid transporter |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (28/31); total (28/31) |
TTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAAT > NC_000913/3980815‑3981083
|
tttGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑gg > 2:22171/1‑139 (MQ=255)
aCCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGt > 2:1168910/1‑139 (MQ=255)
cATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCgg < 2:253214/139‑1 (MQ=255)
tAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGGCACCATTGGCGTCGGCCt > 1:33301/1‑135 (MQ=255)
tAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGGCACCATTGGCGTCGGCCt < 2:33301/135‑1 (MQ=255)
tAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGc > 2:1466904/1‑132 (MQ=255)
tAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGc < 1:1466904/132‑1 (MQ=255)
ggTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATgggg < 1:301729/139‑1 (MQ=255)
ggTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATgggg > 2:301729/1‑139 (MQ=255)
gTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATggggg > 1:823518/1‑139 (MQ=255)
tAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgc < 1:22171/139‑1 (MQ=255)
cGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATggggg > 2:872072/1‑132 (MQ=255)
cGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATggggg < 1:872072/132‑1 (MQ=255)
ggAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATgggg > 2:524901/1‑130 (MQ=255)
ggAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATgggg < 1:524901/130‑1 (MQ=255)
gAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTa > 2:780288/1‑139 (MQ=255)
gAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTa > 1:432265/1‑139 (MQ=255)
aaGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTg < 1:396036/118‑1 (MQ=255)
aaGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTg > 2:396036/1‑118 (MQ=255)
tGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGaa < 2:269816/139‑1 (MQ=255)
cAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAAt < 2:432265/139‑1 (MQ=255)
aacacaacccGAGGAATTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGGAGGTCGACATATCGAACTCAACGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgg < 2:494785/139‑1 (MQ=255)
caacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATg > 2:411147/1‑111 (MQ=255)
caacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATg < 1:411147/111‑1 (MQ=255)
cacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggc > 1:494785/1‑139 (MQ=255)
acGAGGATCTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggcc < 2:1288934/139‑1 (MQ=255)
tATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAtt > 2:999880/1‑139 (MQ=255)
aTGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttg < 2:422434/139‑1 (MQ=255)
gATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa < 2:667210/101‑1 (MQ=255)
gATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa > 1:667210/1‑101 (MQ=255)
gATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGcc < 1:999880/139‑1 (MQ=255)
gATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGcc > 1:1345746/1‑139 (MQ=255)
tAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGcc > 2:603106/1‑137 (MQ=255)
tAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGcc < 1:603106/137‑1 (MQ=255)
cAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTatat < 2:229099/139‑1 (MQ=255)
agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCg > 2:1386834/1‑139 (MQ=255)
aGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGt < 1:1168910/139‑1 (MQ=255)
tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGt < 2:823518/139‑1 (MQ=255)
tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGt > 2:1498641/1‑139 (MQ=255)
tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑ggg > 2:125397/1‑39 (MQ=255)
tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑ggg < 1:125397/39‑1 (MQ=255)
ggCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCt < 1:1498641/139‑1 (MQ=255)
gCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGcc > 1:1061696/1‑57 (MQ=255)
gCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGcc < 2:1061696/57‑1 (MQ=255)
gAAGCTCGACATATCGATCTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgtt < 2:1466053/105‑1 (MQ=255)
gAAGCTCGACATATCGATCTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgtt > 1:1466053/1‑105 (MQ=255)
gAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATc > 1:498795/1‑96 (MQ=255)
gAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATc < 2:498795/96‑1 (MQ=255)
aaGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTtca > 1:1121291/1‑139 (MQ=255)
gCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTtcatc < 2:1345746/139‑1 (MQ=255)
cTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTtcatca < 1:1386834/139‑1 (MQ=255)
gAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgt < 1:136569/89‑1 (MQ=255)
gAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgt > 2:136569/1‑89 (MQ=255)
aCTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATgggg < 1:69761/47‑1 (MQ=255)
aCTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATgggg > 2:69761/1‑47 (MQ=255)
aCTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATggg < 2:389520/139‑1 (MQ=255)
aTCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa < 1:632636/50‑1 (MQ=255)
aTCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa > 2:632636/1‑50 (MQ=255)
tCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggc > 1:879655/1‑65 (MQ=255)
tCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggc < 2:879655/65‑1 (MQ=255)
cGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAAt > 2:1196359/1‑139 (MQ=255)
cGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAAt > 2:1198147/1‑139 (MQ=255)
|
TTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAAT > NC_000913/3980815‑3981083
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
