Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A14 F1 I1 R1 | 770 | 68.5 | 3470224 | 91.7% | 3182195 | 103.4 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,980,957 | (G)6→7 | coding (71/1386 nt) | yifK → | putative APC family amino acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,980,951 | 1 | . | G | 98.6% | 256.3 / ‑3.7 | 72 | coding (65/1386 nt) | yifK | putative APC family amino acid transporter |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (36/35); total (37/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.98e-01 | |||||||||||
ATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑GGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCG > NC_000913/3980814‑3981079 | aTTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑g > 1:961186/1‑139 (MQ=255) cTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATATATGGCAGATAACAAAACAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑gggg‑gggcacc > 2:561123/1‑134 (MQ=255) tCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCa > 2:461143/1‑139 (MQ=255) tACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCg < 1:461143/139‑1 (MQ=255) tAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACATCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGcc > 2:1243370/1‑139 (MQ=255) aaCGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCt > 2:1683665/1‑139 (MQ=255) aaCGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCt > 1:972429/1‑139 (MQ=255) gATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGtt > 2:42003/1‑139 (MQ=255) tAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTa > 1:1114135/1‑139 (MQ=255) aaCCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTAt > 1:409965/1‑139 (MQ=255) aCCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATg < 2:1148330/139‑1 (MQ=255) ccGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGc < 2:165777/129‑1 (MQ=255) ccGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGc > 1:165777/1‑129 (MQ=255) ccGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa < 2:755541/139‑1 (MQ=255) ggAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGt > 2:159308/1‑139 (MQ=255) aaGCATGTAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAcc > 2:391767/1‑139 (MQ=255) aaGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAcc < 2:1114135/139‑1 (MQ=255) tGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAcc < 1:1703742/134‑1 (MQ=255) tGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAcc > 2:1703742/1‑134 (MQ=255) tGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGCGGCCGCCAGTACCCTGaa < 2:961186/139‑1 (MQ=255) gCAAACACAAAACAAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGaaa > 2:1162089/1‑139 (MQ=255) aaaCACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCg < 2:1013118/102‑1 (MQ=255) aaaCACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCg > 1:1013118/1‑102 (MQ=255) cacaacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggc < 1:159308/139‑1 (MQ=255) acaacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggcc < 1:463301/139‑1 (MQ=255) caacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa > 2:1215748/1‑121 (MQ=255) caacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa < 1:1215748/121‑1 (MQ=255) cacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggc > 1:133095/1‑139 (MQ=255) gATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCg < 1:793410/139‑1 (MQ=255) tttATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa > 2:547058/1‑110 (MQ=255) tttATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa < 1:547058/110‑1 (MQ=255) aTGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttg < 1:1243370/139‑1 (MQ=255) aTGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttg < 2:409965/139‑1 (MQ=255) ggCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGc > 2:1706997/1‑86 (MQ=255) ggCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGc < 1:1706997/86‑1 (MQ=255) gATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa < 1:307654/101‑1 (MQ=255) gATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa > 2:307654/1‑101 (MQ=255) gATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGcc > 1:1449382/1‑139 (MQ=255) aTAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGgccgc < 2:11312/98‑1 (MQ=255) aTAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGgccgc > 1:11312/1‑98 (MQ=255) aTAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATggg > 2:271229/1‑115 (MQ=255) aTAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATggg < 1:271229/115‑1 (MQ=255) agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa < 2:1584928/88‑1 (MQ=255) agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa > 1:1584928/1‑88 (MQ=255) agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCg > 2:489111/1‑139 (MQ=255) cAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttg < 2:1116713/115‑1 (MQ=255) cAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttg > 1:1116713/1‑115 (MQ=255) cAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCg < 1:1476476/134‑1 (MQ=255) cAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCg > 2:1476476/1‑134 (MQ=255) gCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCt < 2:718141/120‑1 (MQ=255) gCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCt > 1:718141/1‑120 (MQ=255) gCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGtt > 2:1231378/1‑139 (MQ=255) tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGc < 2:1672751/59‑1 (MQ=255) tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGc > 1:1672751/1‑59 (MQ=255) tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa < 2:934819/78‑1 (MQ=255) tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa > 1:934819/1‑78 (MQ=255) gCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGcc > 2:515361/1‑57 (MQ=255) gCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGcc < 1:515361/57‑1 (MQ=255) ggAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGc > 1:41595/1‑53 (MQ=255) ggAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGc < 2:41595/53‑1 (MQ=255) ggAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCttttt < 2:701666/139‑1 (MQ=255) cTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTtcatca < 1:489111/139‑1 (MQ=255) cGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCatgatg > 2:667024/1‑139 (MQ=255) tatCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATgggg < 1:246639/53‑1 (MQ=255) tatCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATgggg > 2:246639/1‑53 (MQ=255) atCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGc > 1:1527921/1‑40 (MQ=255) atCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGc < 2:1527921/40‑1 (MQ=255) tCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCg < 2:133095/139‑1 (MQ=255) aTCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgg > 1:263253/1‑69 (MQ=255) aTCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgg < 2:263253/69‑1 (MQ=255) tcacccT‑GGGGAGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAGCCTGAAATgggccgggc > 2:1005390/4‑70 (MQ=255) tCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggc < 1:1005390/70‑1 (MQ=255) cGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATggg > 1:1571316/1‑133 (MQ=255) cGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATggg < 2:1571316/133‑1 (MQ=255) | ATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑GGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCG > NC_000913/3980814‑3981079 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |