Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I1 R1	748	32.0	1685426	92.6%	1560704	103.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,835,045	G→A	378 (TAG→TAA)	norW →	NADH:flavorubredoxin oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,835,045	0	G	A	100.0%	77.5 / NA	25	378 (TAG→TAA)	norW	NADH:flavorubredoxin oxidoreductase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (12/13); total (12/13)

ACCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGT  >  NC_000913/2834926‑2835156

aCCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTaaa                                                                                              >  2:715387/1‑139 (MQ=255)

                 ggtggCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCt                                                                             >  1:123716/1‑139 (MQ=255)

                      cgcgcgGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGgcgc                                                                        >  2:755712/1‑139 (MQ=255)

                       gcgcgGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGcgcg                                                                       >  2:106472/1‑139 (MQ=255)

                                tGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCg                                                              <  1:106472/139‑1 (MQ=255)

                                     aTGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTa                                                         <  1:755712/139‑1 (MQ=255)

                                         tGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACggg                                                     <  2:320334/139‑1 (MQ=255)

                                            ccAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTggg                                                                                                            <  1:209206/81‑1 (MQ=255)

                                            ccAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTggg                                                                                                            >  2:209206/1‑81 (MQ=255)

                                                   cgcgCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCAtgtg                                           <  2:422512/139‑1 (MQ=255)

                                                                  aGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAGATGTCGGATg                                                                                    <  1:242888/83‑1 (MQ=255)

                                                                  aGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATg                                                                                    >  2:242888/1‑83 (MQ=255)

                                                                              aTGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTgg                                                                           <  1:394975/80‑1 (MQ=255)

                                                                              aTGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTgg                                                                           >  2:394975/1‑80 (MQ=255)

                                                                              aTGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTa                                                         >  1:712573/1‑98 (MQ=255)

                                                                              aTGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTa                                                         <  2:712573/98‑1 (MQ=255)

                                                                                        cATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACggg                                                     >  1:741232/1‑92 (MQ=255)

                                                                                        cATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACggg                                                     <  2:741232/92‑1 (MQ=255)

                                                                                            tGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGgtgt  <  1:91390/139‑1 (MQ=255)

                                                                                                    tgAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCa                >  1:580862/1‑117 (MQ=255)

                                                                                                    tgAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCa                <  2:580862/117‑1 (MQ=255)

                                                                                                     gAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGc                                      >  2:110765/1‑94 (MQ=255)

                                                                                                     gAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGc                                      <  1:110765/94‑1 (MQ=255)

                                                                                                             tGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGAt                                 >  2:567233/1‑91 (MQ=255)

                                                                                                             tGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGAt                                 <  1:567233/91‑1 (MQ=255)

ACCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGT  >  NC_000913/2834926‑2835156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A