Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	762	35.5	1545288	95.5%	1475750	117.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,835,045	G→A	378 (TAG→TAA)	norW →	NADH:flavorubredoxin oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,835,045	0	G	A	100.0%	78.2 / NA	25	378 (TAG→TAA)	norW	NADH:flavorubredoxin oxidoreductase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (12/13); total (12/13)

GCTGGCAAATTAATACCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGTTTTAT  >  NC_000913/2834912‑2835176

gCTGGCAAATTAATACCGAACGCCAGGGAATGGTGGCGCGCGGCGTNGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTggg                                                                                                                                <  1:593259/139‑1 (MQ=255)

   ggCAAATTAATACCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTa                                                                                                                             <  1:593769/139‑1 (MQ=255)

   ggCAAATTAATACCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTCGATTGTTGAAAACATTGCCGATGTAAGTGGGCTa                                                                                                                             >  2:762136/1‑139 (MQ=255)

    gCAAATTAATACCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTAc                                                                                                                            <  2:221150/139‑1 (MQ=255)

     cAAATTAATACCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACt                                                                                                                           <  2:680667/139‑1 (MQ=255)

                            aaTGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGAc                                                                                                    >  1:316780/1‑139 (MQ=255)

                                    cgcgcgGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGgcgc                                                                                            <  2:459281/139‑1 (MQ=255)

                                      cgcgGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGt                                                                                          <  2:669822/139‑1 (MQ=255)

                                                       tGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTCGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACggg                                                                         <  1:762136/139‑1 (MQ=255)

                                                              cTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTg                                                                                                <  2:161203/109‑1 (MQ=255)

                                                              cTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTg                                                                                                >  1:161203/1‑109 (MQ=255)

                                                                                aGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGc                                                >  1:132766/1‑139 (MQ=255)

                                                                                  tGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGt                                              >  1:610771/1‑139 (MQ=255)

                                                                                  tGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGt                                              >  1:761053/1‑139 (MQ=255)

                                                                                  tGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGt                                              >  2:109181/1‑139 (MQ=255)

                                                                                        tCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTAcgc                                        >  1:508823/1‑139 (MQ=255)

                                                                                            aTGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTAc                                                                                                                            <  2:442578/51‑1 (MQ=255)

                                                                                            aTGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTAc                                                                                                                            >  1:463135/1‑51 (MQ=255)

                                                                                            aTGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTAc                                                                                                                            <  2:463135/51‑1 (MQ=255)

                                                                                            aTGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTAc                                                                                                                            >  1:442578/1‑51 (MQ=255)

                                                                                            aTGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCa                                    >  1:771440/1‑139 (MQ=255)

                                                                                                 agagGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg                               >  1:448075/1‑139 (MQ=255)

                                                                                                    ggCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCaa                            <  2:132766/139‑1 (MQ=255)

                                                                                                      cATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATg                          <  1:647765/139‑1 (MQ=255)

                                                                                                                              cGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGTTTTAt  <  2:610771/139‑1 (MQ=255)

GCTGGCAAATTAATACCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGTTTTAT  >  NC_000913/2834912‑2835176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A