Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I1 R1	776	60.0	3074904	90.1%	2770488	104.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,835,045	G→A	378 (TAG→TAA)	norW →	NADH:flavorubredoxin oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,835,045	0	G	A	97.6%	126.2 / ‑4.8	42	378 (TAG→TAA)	norW	NADH:flavorubredoxin oxidoreductase
Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (24/17); total (25/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

ACCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AG‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGTTTTAT  >  NC_000913/2834926‑2835176

aCCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTaaa                                                                                                                  >  2:792767/1‑139 (MQ=255)

    aaCGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGt                                                                                                              >  1:713200/1‑139 (MQ=255)

     aCGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGTGGGCTACTGTGCCTAAAATg                                                                                                               >  1:1296115/1‑139 (MQ=255)

     aCGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTc                                                                                                             >  1:911258/1‑139 (MQ=255)

               aTGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACg                                                                                                   <  2:911258/139‑1 (MQ=255)

               aTGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACg                                                                                                   <  2:713200/139‑1 (MQ=255)

                     gcgcgcGGCGTTGACGATGCTGACCAGCTTCCCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGAATGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGgcg                                                                                             >  2:258440/1‑139 (MQ=255)

                        cgcgGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGt                                                                                          >  1:802669/1‑139 (MQ=255)

                        cgcgGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGt                                                                                          >  2:612895/1‑139 (MQ=255)

                           ggcgTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCtt                                                                                       <  1:792767/139‑1 (MQ=255)

                                      tGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTAc                                                                            >  2:1038248/1‑139 (MQ=255)

                                             cAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACg                                                                     <  1:612895/139‑1 (MQ=255)

                                               gCTTCGCGCCTTTGTGGTCAGTGAGGATCAGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCa                                                                   <  1:1038248/139‑1 (MQ=255)

                                                cTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCAt                                                                  >  1:939207/1‑139 (MQ=255)

                                                                  aGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCt                                                                              <  1:1336997/109‑1 (MQ=255)

                                                                  aGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCt                                                                              >  2:1336997/1‑109 (MQ=255)

                                                                    tGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGt                                              >  2:304779/1‑139 (MQ=255)

                                                                    tGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGt                                              >  2:1258728/1‑139 (MQ=255)

                                                                    tGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGACCGGATAAGGCGt                                              >  1:234587/1‑139 (MQ=255)

                                                                       ggATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTa                                           >  2:1137969/1‑139 (MQ=255)

                                                                         aTCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTAcg                                         <  1:1258728/139‑1 (MQ=255)

                                                                            ggATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTAcgccg                                      >  1:1512805/1‑139 (MQ=255)

                                                                             gATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTAcgccgc                                     >  1:125189/1‑139 (MQ=255)

                                                                              aTGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAgg                                                           <  2:1527826/116‑1 (MQ=255)

                                                                              aTGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAgg                                                           >  1:1527826/1‑116 (MQ=255)

                                                                              aTGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCa                                    <  1:1137969/139‑1 (MQ=255)

                                                                                   agagGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATcc                                                                                   >  1:492794/1‑87 (MQ=255)

                                                                                   agagGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATcc                                                                                   <  2:492794/87‑1 (MQ=255)

                                                                                     agGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGa                                                                       <  1:377422/97‑1 (MQ=255)

                                                                                     agGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGa                                                                       >  2:377422/1‑97 (MQ=255)

                                                                                      ggCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCaa                            <  1:304779/139‑1 (MQ=255)

                                                                                           ttGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGcgcg                                                                                           >  2:1428972/1‑71 (MQ=255)

                                                                                           ttGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGcgcg                                                                                           <  1:1428972/71‑1 (MQ=255)

                                                                                            tGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACg                                                                     >  2:183874/1‑92 (MQ=255)

                                                                                            tGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACg                                                                     <  1:183874/92‑1 (MQ=255)

                                                                                                           aTTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGAc                                                                                                    >  2:1317755/1‑46 (MQ=255)

                                                                                                           aTTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGAc                                                                                                    <  1:1317755/46‑1 (MQ=255)

                                                                                                           aTTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGt       >  2:484272/1‑139 (MQ=255)

                                                                                                             tGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGttt     >  1:545642/1‑139 (MQ=255)

                                                                                                             tGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGttt     >  1:175844/1‑139 (MQ=255)

                                                                                                                cGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGTTTTAt  <  1:258440/139‑1 (MQ=255)

                                                                                                                cGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGTTTTAt  <  2:939207/139‑1 (MQ=255)

ACCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AG‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGTTTTAT  >  NC_000913/2834926‑2835176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A