Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,835,045	G→A	378 (TAG→TAA)	norW →	NADH:flavorubredoxin oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,835,045	0	G	A	100.0%	95.2 / NA	30	378 (TAG→TAA)	norW	NADH:flavorubredoxin oxidoreductase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/14); total (16/14)

GGCAAATTAATACCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGTTTTAT  >  NC_000913/2834915‑2835176

ggCAAATTAATACCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTa                                                                                                                             >  1:797821/1‑139 (MQ=255)

 gCAAATTAATACCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTAc                                                                                                                            <  2:723321/139‑1 (MQ=255)

  cAAATTAATACCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACt                                                                                                                           <  1:441665/139‑1 (MQ=255)

               aaCGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGt                                                                                                              >  2:893431/1‑139 (MQ=255)

                aCGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTc                                                                                                             >  2:652721/1‑139 (MQ=255)

                       ggAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCg                                                                                                      >  2:447097/1‑139 (MQ=255)

                              tggCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTgg                                                                                               >  2:670119/1‑139 (MQ=255)

                                         gTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGGCGCTGGCGCGTCTTATc                                                                                    <  1:661553/139‑1 (MQ=255)

                                         gTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATc                                                                                    <  2:794354/139‑1 (MQ=255)

                                               gATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCt                                                                              <  2:797821/139‑1 (MQ=255)

                                                           cTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCAt                                                                  >  1:398962/1‑139 (MQ=255)

                                                                cgcCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTa                                                             >  1:235508/1‑139 (MQ=255)

                                                                     ttGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCg                                                        >  2:991637/1‑139 (MQ=255)

                                                                             aGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTAc                                                                                                                            >  2:1049971/1‑63 (MQ=255)

                                                                             aGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTAc                                                                                                                            <  1:1049971/63‑1 (MQ=255)

                                                                               tGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTTTc                                                                                    <  2:613071/101‑1 (MQ=255)

                                                                               tGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATc                                                                                    >  1:613071/1‑101 (MQ=255)

                                                                                    aTCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTAcg                                         >  1:205233/1‑139 (MQ=255)

                                                                                        gATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTAcgccgc                                     >  2:736494/1‑139 (MQ=255)

                                                                                         aTGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTAc                                                                                                                            >  1:601389/1‑51 (MQ=255)

                                                                                         aTGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTAc                                                                                                                            <  2:601389/51‑1 (MQ=255)

                                                                                             aaGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCg                                >  2:575982/1‑139 (MQ=255)

                                                                                              agagGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGgc                                                                                              <  1:187246/76‑1 (MQ=255)

                                                                                              agagGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGgc                                                                                              >  2:187246/1‑76 (MQ=255)

                                                                                                agGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCa                             <  1:991637/139‑1 (MQ=255)

                                                                                                   cATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATg                          <  1:893431/139‑1 (MQ=255)

                                                                                                   cATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATg                          <  1:588888/139‑1 (MQ=255)

                                                                                                         gATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTcc                    <  1:575982/139‑1 (MQ=255)

                                                                                                                        tGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGttt     >  1:303181/1‑139 (MQ=255)

                                                                                                                           cGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGTTTTAt  <  1:867237/139‑1 (MQ=255)

GGCAAATTAATACCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGTTTTAT  >  NC_000913/2834915‑2835176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A