Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I1 R1	748	32.0	1685426	92.6%	1560704	103.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,129,036	G→A	255 (TAG→TAA)	glcC →	glycolate‑inducible glc operon transcriptional repressor, autorepressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,129,036	0	G	A	100.0%	151.9 / NA	48	255 (TAG→TAA)	glcC	glycolate‑inducible glc operon transcriptional repressor, autorepressor
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (25/23); total (25/23)

TGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGACATCTCCAGCGCATCGATCTTCCGG  >  NC_000913/3128900‑3129166

tGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCGACCATTTGATTCGCTCGGCGGTGCCTCTGGAGATGAACCTGAGTTAACt                                                                                                                                  <  2:801873/139‑1 (MQ=255)

       gCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTa                                                                                                                           >  1:808549/1‑139 (MQ=255)

       gCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTa                                                                                                                           >  2:629590/1‑139 (MQ=255)

       gCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTa                                                                                                                           >  1:497051/1‑139 (MQ=255)

                     cccAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCAt                                                                                                             >  1:400161/1‑139 (MQ=255)

                              aagcaCGCGAGCATATGCGGACCGTGAAAAGGAATCTCCACGTGATCGAGCTGGAAGGCCACCATTTGGTTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTCCATACAATCCGt                                                                                                    <  2:213474/138‑1 (MQ=255)

                                                ggACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCg                                                                                  <  2:13514/139‑1 (MQ=255)

                                                  aCCGTGAAAAAGAATCTCGACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGcc                                                                                >  2:465744/1‑139 (MQ=255)

                                                  aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACt                                                                                                                                  >  2:164529/1‑89 (MQ=255)

                                                  aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCt                                                                                                                      <  1:772424/101‑1 (MQ=255)

                                                  aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCt                                                                                                                      >  2:772424/1‑101 (MQ=255)

                                                  aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACa                                                                                                          <  1:717726/113‑1 (MQ=255)

                                                  aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACa                                                                                                          >  2:717726/1‑113 (MQ=255)

                                                  aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAAt                                                                                                        <  2:513066/115‑1 (MQ=255)

                                                  aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAAt                                                                                                        >  1:513066/1‑115 (MQ=255)

                                                  aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGt                                                                                                    >  1:643954/1‑119 (MQ=255)

                                                  aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGt                                                                                                    <  2:643954/119‑1 (MQ=255)

                                                  aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGcc                                                                                >  2:335112/1‑139 (MQ=255)

                                                  aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGcc                                                                                >  1:729319/1‑139 (MQ=255)

                                                    cGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGt                                                                                                                               <  2:95315/90‑1 (MQ=255)

                                                    cGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGt                                                                                                                               >  1:95315/1‑90 (MQ=255)

                                                      tGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGCGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCaa                                                                            >  2:541953/1‑139 (MQ=255)

                                                      tGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGc                                                                                                                    <  1:647163/99‑1 (MQ=255)

                                                      tGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGc                                                                                                                    >  2:647163/1‑99 (MQ=255)

                                                      tGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCaa                                                                            <  1:238453/139‑1 (MQ=255)

                                                                tctcCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTCCCAg                                                                  >  2:711397/1‑139 (MQ=255)

                                                                  tcCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCa                                                                <  2:729319/139‑1 (MQ=255)

                                                                  tcCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCa                                                                <  1:335112/139‑1 (MQ=255)

                                                                              aGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCt                                                                                                                   <  2:32435/76‑1 (MQ=255)

                                                                              aGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCt                                                                                                                   >  1:32435/1‑76 (MQ=255)

                                                                              aGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGc                                                    >  2:714525/1‑139 (MQ=255)

                                                                                             aTTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGa                                     <  1:714525/139‑1 (MQ=255)

                                                                                             aTTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGa                                     <  2:497051/139‑1 (MQ=255)

                                                                                              tttGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTa                                                                                          >  1:77551/1‑85 (MQ=255)

                                                                                              tttGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTa                                                                                          <  2:77551/85‑1 (MQ=255)

                                                                                                      gCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGc                                                                                                                    >  1:474654/1‑51 (MQ=255)

                                                                                                      gCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGc                                                                                                                    <  2:474654/51‑1 (MQ=255)

                                                                                                       cTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAAt                                                                                                                        >  2:179402/1‑46 (MQ=255)

                                                                                                       cTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAAt                                                                                                                        <  1:179402/46‑1 (MQ=255)

                                                                                                        tCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACgg                                                                                       >  1:84174/1‑78 (MQ=255)

                                                                                                        tCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACgg                                                                                       <  2:84174/78‑1 (MQ=255)

                                                                                                                    cTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCa                                                                >  2:161238/1‑89 (MQ=255)

                                                                                                                    cTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCa                                                                <  1:161238/89‑1 (MQ=255)

                                                                                                                        agaTGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTa                                               >  1:357933/1‑102 (MQ=255)

                                                                                                                        agaTGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTa                                               <  2:357933/102‑1 (MQ=255)

                                                                                                                               ccTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCAt                             <  2:551689/113‑1 (MQ=255)

                                                                                                                               ccTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCAt                             >  1:551689/1‑113 (MQ=255)

                                                                                                                                cTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGACATCTCCAGCGCATCGATCTTCcgg  <  1:513164/139‑1 (MQ=255)

TGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGACATCTCCAGCGCATCGATCTTCCGG  >  NC_000913/3128900‑3129166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A