Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	765	46.1	2269152	93.8%	2128464	105.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,129,036	G→A	255 (TAG→TAA)	glcC →	glycolate‑inducible glc operon transcriptional repressor, autorepressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,129,036	0	G	A	100.0%	128.4 / NA	40	255 (TAG→TAA)	glcC	glycolate‑inducible glc operon transcriptional repressor, autorepressor
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/23); total (17/23)

GCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGACATCTCCAGCGCATCGATCTTCCGGCGGG  >  NC_000913/3128907‑3129170

gCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTa                                                                                                                               >  2:370389/1‑139 (MQ=255)

  tGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGACCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTaaa                                                                                                                             >  2:217434/1‑139 (MQ=255)

         cGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCtt                                                                                                                      <  1:791772/139‑1 (MQ=255)

              cccAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGACTCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCt                                                                                                                       >  2:1026012/1‑133 (MQ=255)

              cccAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCt                                                                                                                       <  1:1026012/133‑1 (MQ=255)

                      gcagcaCGCGATCATGTGTGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACt                                                                                                                                      >  1:653307/1‑110 (MQ=255)

                      gcagcaCGCGATCATGTGTGGACCGTGAAAAAGAATCTCCACGATATCGAGCGGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACt                                                                                                                                      <  2:653307/110‑1 (MQ=255)

                         gcaCGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTaa                                                                                                      <  1:882427/139‑1 (MQ=255)

                                      tgCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACt                                                                                                                                      >  2:986369/1‑94 (MQ=255)

                                      tgCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCt                                                                                         <  1:330144/139‑1 (MQ=255)

                                      tgCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCt                                                                                         <  1:595099/139‑1 (MQ=255)

                                       gCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTc                                                                                        <  1:370389/139‑1 (MQ=255)

                                           aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGcc                                                                                    >  2:257207/1‑139 (MQ=255)

                                           aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGcc                                                                                    >  1:838146/1‑139 (MQ=255)

                                           aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGCACGGCTCCGcc                                                                                    >  1:734874/1‑139 (MQ=255)

                                               tGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACt                                                                                                                                      <  1:657953/85‑1 (MQ=255)

                                               tGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCt                                                                                         >  2:185567/1‑130 (MQ=255)

                                               tGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCt                                                                                         <  1:185567/130‑1 (MQ=255)

                                                 aaaaaGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATg                                                                              <  2:838146/139‑1 (MQ=255)

                                                              aCGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTaa                                                                                                      >  2:231917/1‑102 (MQ=255)

                                                              aCGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTaa                                                                                                      <  1:231917/102‑1 (MQ=255)

                                                                  tatCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTAtt                                                             <  2:461030/139‑1 (MQ=255)

                                                                  tatCGAGCTGGAAGACCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTAtt                                                             <  1:217434/139‑1 (MQ=255)

                                                                   atCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTc                                                            <  1:145297/139‑1 (MQ=255)

                                                                     cGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTAt                                                                                                                                 >  2:1016570/1‑68 (MQ=255)

                                                                     cGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTAt                                                                                                                                 <  1:1016570/68‑1 (MQ=255)

                                                                            gAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTa                                                   >  1:307431/1‑139 (MQ=255)

                                                                                gCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACa                                                                                                              >  1:1097986/1‑76 (MQ=255)

                                                                                gCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACa                                                                                                              <  2:1097986/76‑1 (MQ=255)

                                                                                         tGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGa                                      <  2:307431/139‑1 (MQ=255)

                                                                                          gATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGAt                                     <  1:257207/139‑1 (MQ=255)

                                                                                               gCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCa                                                                       <  1:153676/100‑1 (MQ=255)

                                                                                               gCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCa                                                                       >  2:153676/1‑100 (MQ=255)

                                                                                                                  gaTGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGACATCTCCAGCGCATCGAt             >  2:517137/1‑139 (MQ=255)

                                                                                                                           gAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGTTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGACATCTCCAGCGCATCGATCTTCcggcg    <  2:285605/139‑1 (MQ=255)

                                                                                                                           gAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTa                                                   <  1:631549/92‑1 (MQ=255)

                                                                                                                           gAGTTAACTGGTATTAAAGCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTa                                                   >  2:631549/1‑92 (MQ=255)

                                                                                                                            aGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTc                                           >  2:24385/1‑99 (MQ=255)

                                                                                                                            aGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTc                                           <  1:24385/99‑1 (MQ=255)

                                                                                                                             gTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGACATCTCCAGCGCATCGATCTTCCGGCggg  <  1:176144/139‑1 (MQ=255)

GCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGACATCTCCAGCGCATCGATCTTCCGGCGGG  >  NC_000913/3128907‑3129170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A