Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,129,036	G→A	255 (TAG→TAA)	glcC →	glycolate‑inducible glc operon transcriptional repressor, autorepressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,129,036	0	G	A	100.0%	146.8 / NA	46	255 (TAG→TAA)	glcC	glycolate‑inducible glc operon transcriptional repressor, autorepressor
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (23/23); total (23/23)

GCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGACATCTCCAGCGCATCGATCTTCCGG  >  NC_000913/3128907‑3129166

gCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTa                                                                                                                             >  2:279238/1‑139 (MQ=255)

gCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTa                                                                                                                             >  1:251727/1‑139 (MQ=255)

     cGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCt                                                                                                                        <  2:738980/139‑1 (MQ=255)

              cccAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCAt                                                                                                               >  1:739526/1‑139 (MQ=255)

              cccAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCAt                                                                                                               >  1:581920/1‑139 (MQ=255)

                       cagcaCGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGt                                                                                                      <  2:24002/139‑1 (MQ=255)

                       cagcaCGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGt                                                                                                      <  2:948450/139‑1 (MQ=255)

                             gcgATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGc                                                                                                 >  1:77441/1‑138 (MQ=255)

                             gcgATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGc                                                                                                 <  2:77441/138‑1 (MQ=255)

                               gATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑Tg                                                                                            >  1:294126/1‑139 (MQ=255)

                                      tgCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCt                                                                                     <  2:440010/139‑1 (MQ=255)

                                       gCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGtgtacggc                                                                                    >  1:112820/1‑132 (MQ=255)

                                        cGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTcc                                                                                   <  2:581920/139‑1 (MQ=255)

                                           aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTa                                                                                                     <  1:913786/120‑1 (MQ=255)

                                           aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTa                                                                                                     >  2:913786/1‑120 (MQ=255)

                                           aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGcc                                                                                >  1:553285/1‑139 (MQ=255)

                                           aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGcc                                                                                >  1:511390/1‑139 (MQ=255)

                                           aCCGTGAAAAAGAATCTCCACGATATCGAGCCGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGcc                                                                                >  1:956470/1‑139 (MQ=255)

                                               tGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCaa                                                                            >  2:857340/1‑139 (MQ=255)

                                               tGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCaa                                                                            >  2:700146/1‑139 (MQ=255)

                                               tGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCaa                                                                            >  2:125789/1‑139 (MQ=255)

                                               tGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGA‑GAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAAt                                                                           >  2:1053900/1‑139 (MQ=255)

                                                gAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAAt                                                                           <  1:112260/139‑1 (MQ=255)

                                                        aTCTCCACGATATCGAGCCGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCa                                                                   <  2:956470/139‑1 (MQ=255)

                                                         tctcCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCAg                                                                  <  2:17334/139‑1 (MQ=255)

                                                             cACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCAGCATg                                                              <  2:17888/139‑1 (MQ=255)

                                                                     cGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCAt                                                      <  1:279238/139‑1 (MQ=255)

                                                                            gAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTa                                               >  1:224790/1‑139 (MQ=255)

                                                                             aaGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTAt                                              <  2:224790/139‑1 (MQ=255)

                                                                                  caccaTTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCt                                                                                                                        >  2:960407/1‑62 (MQ=255)

                                                                                  caccaTTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCt                                                                                                                        <  1:960407/62‑1 (MQ=255)

                                                                                    ccaTTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTc                                       >  1:264957/1‑139 (MQ=255)

                                                                                      aTTTGATTCGCTCGGCGGTGCCGCTGGATATGAACCTGAGTTAACTGGTATTACATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCACTGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGa                                     <  2:511390/139‑1 (MQ=255)

                                                                                      aTTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGa                                     <  1:92041/139‑1 (MQ=255)

                                                                                         tGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAg                                    <  2:112820/139‑1 (MQ=255)

                                                                                               gCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTAtt                                             >  1:94818/1‑122 (MQ=255)

                                                                                               gCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTAtt                                             <  2:94818/122‑1 (MQ=255)

                                                                                               gCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATg                            >  2:530821/1‑139 (MQ=255)

                                                                                                cTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCa                                                       >  1:857489/1‑111 (MQ=255)

                                                                                                cTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACGGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCa                                                       <  2:857489/111‑1 (MQ=255)

                                                                                                     cggTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCa                                                                   <  1:935319/94‑1 (MQ=255)

                                                                                                     cggTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCa                                                                   >  2:935319/1‑94 (MQ=255)

                                                                                                                   aTGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGACATCTCCAGCGCATCGATc        <  2:251727/139‑1 (MQ=255)

                                                                                                                         cTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGACATCTCCAGCGCATCGATCTTCcgg  <  2:403834/139‑1 (MQ=255)

                                                                                                                            aGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCAGCATg                                                              <  2:656926/76‑1 (MQ=255)

                                                                                                                            aGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCAGCATg                                                              >  1:656926/1‑76 (MQ=255)

GCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGACATCTCCAGCGCATCGATCTTCCGG  >  NC_000913/3128907‑3129166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A