Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I1 R1	778	68.7	3228504	93.4%	3015422	109.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,220,190	G→A	A233T (GCT→ACT)	patA →	putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,220,190	0	G	A	100.0%	148.0 / NA	46	A233T (GCT→ACT)	patA	putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/26); total (20/26)

GGCCACGGCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGGCTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATG  >  NC_000913/3220060‑3220324

ggCCACGGCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTg                                                                                                                                <  1:629072/139‑1 (MQ=255)

 gCCACGGCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGa                                                                                                                               >  1:1421894/1‑139 (MQ=255)

        cGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGa                                                                                                                        >  2:506274/1‑139 (MQ=255)

         gAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGaa                                                                                                                       <  2:88959/139‑1 (MQ=255)

         gAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGaa                                                                                                                       <  1:1316592/139‑1 (MQ=255)

               gACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGAtt                                                                                                                 <  1:209971/139‑1 (MQ=255)

                aCCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTc                                                                                                                <  1:506274/139‑1 (MQ=255)

                               tttATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCg                                                                                                 >  2:1461429/1‑139 (MQ=255)

                               tttATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCg                                                                                                 >  2:569178/1‑139 (MQ=255)

                                             gCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTgccgccg                                                                                   <  1:881402/139‑1 (MQ=255)

                                                      ccGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTAt                                                                          >  2:679509/1‑139 (MQ=255)

                                                             gtgCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCAccg                                                                   <  1:1461429/139‑1 (MQ=255)

                                                              tgCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCAccgc                                                                  <  2:806647/139‑1 (MQ=255)

                                                              tgCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCAccgc                                                                  <  1:569178/139‑1 (MQ=255)

                                                                ccGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCAccgccg                                                                >  2:1273480/1‑139 (MQ=255)

                                                                ccGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCAccgccg                                                                >  2:1142303/1‑139 (MQ=255)

                                                                   tttGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCcgtac                                                             >  1:1228805/1‑139 (MQ=255)

                                                                   tttGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCcgtac                                                             >  2:227636/1‑139 (MQ=255)

                                                                     tGGCAATATCGAAGCCATGCGCACGGCTGTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAgg                                                                                                      <  1:939706/96‑1 (MQ=255)

                                                                     tGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAgg                                                                                                      >  2:939706/1‑96 (MQ=255)

                                                                       gCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTaa                                                         <  1:1286364/139‑1 (MQ=255)

                                                                                     aTGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGt                                           >  1:299759/1‑139 (MQ=255)

                                                                                      tGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGTGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGtt                                          >  2:1418631/1‑139 (MQ=255)

                                                                                       gcgcACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGAtt                                                                                                                 <  2:195046/67‑1 (MQ=255)

                                                                                       gcgcACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGAtt                                                                                                                 >  1:195046/1‑67 (MQ=255)

                                                                                        cgcACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCg                                        <  1:1063727/139‑1 (MQ=255)

                                                                                         gcACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCgg                                       <  1:1142303/139‑1 (MQ=255)

                                                                                         gcACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCgg                                       <  1:679509/139‑1 (MQ=255)

                                                                                           aCGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGTGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGgcg                                     <  1:1418631/139‑1 (MQ=255)

                                                                                            cGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGgcgc                                    <  1:227636/139‑1 (MQ=255)

                                                                                                     aCGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTgatgat                           <  2:1421894/139‑1 (MQ=255)

                                                                                                        aGTGCAAAAAAACCGGTGATGATGTTACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCt                        <  1:624329/139‑1 (MQ=255)

                                                                                                         gTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTgccgccg                                                                                   >  1:781468/1‑79 (MQ=255)

                                                                                                         gTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTgccgccg                                                                                   <  2:781468/79‑1 (MQ=255)

                                                                                                         gTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGccgccgccg                                                                                <  2:1579317/82‑1 (MQ=255)

                                                                                                         gTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGccgccgccg                                                                                >  1:1579317/1‑82 (MQ=255)

                                                                                                         gTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCgg                                       <  1:625117/123‑1 (MQ=255)

                                                                                                         gTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCgg                                       >  2:625117/1‑123 (MQ=255)

                                                                                                         gTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTc                       >  1:405480/1‑139 (MQ=255)

                                                                                                          tGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGa                                                <  1:1323714/113‑1 (MQ=255)

                                                                                                          tGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGa                                                >  2:1323714/1‑113 (MQ=255)

                                                                                                                      ggTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACtgat                              <  1:127125/119‑1 (MQ=255)

                                                                                                                      ggTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACtgat                              >  2:127125/1‑119 (MQ=255)

                                                                                                                           tgatgTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGa                     <  2:848125/123‑1 (MQ=255)

                                                                                                                           tgatgTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGa                     >  1:848125/1‑123 (MQ=255)

                                                                                                                              tgtgACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATg  <  1:1273480/139‑1 (MQ=255)

GGCCACGGCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGGCTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATG  >  NC_000913/3220060‑3220324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A