Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F1 I2 R1 774 49.2 2112398 96.9% 2046913 118.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913 2,893,961 Δ3 bp coding (43‑45/261 nt) ygcO → putative 4Fe‑4S cluster‑containing protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 28939601 (0.020)44 (0.850) 40/236 0.2 97.8% coding (42/261 nt) ygcO putative 4Fe‑4S cluster‑containing protein
?NC_000913 2893964 = 1 (0.020)coding (46/261 nt) ygcO putative 4Fe‑4S cluster‑containing protein

TAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2893826‑2893960
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACCGGAAGGTAACTTACGTATTGACTATCGCAGTTGCCTGGAGTGTGGCACCTGCC  >  NC_000913/2893964‑2894099
                                                                                                                                                                                                                                                                               
TAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGATTG                                                                                                                                      >  2:720367/1‑139
  AACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGATTGTT                                                                                                                                    >  2:361582/1‑139
   ACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGATTGTTC                                                                                                                                   >  1:894869/1‑139
             CCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGATTGTTCCGGCTGACTC                                                                                                                         >  1:987079/1‑139
              CGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGATTGTTCCGGCTGACTCC                                                                                                                        >  1:1007040/1‑139
                 TGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGATTGTTCCGGCTGACTCCGTT                                                                                                                     <  2:782247/139‑1
                 TGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGATTGTTCCGGCTGACTCCGTT                                                                                                                     >  1:349816/1‑139
                             TCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGATTGTTCCGGCTGACTCCGTTAAGCGCCAGACG                                                                                                         >  1:684419/1‑139
                                    TCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAAC                                                                                                  >  2:637158/1‑139
                                                TCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGATTGTTCCGGCTGACTCCGTTGAGCGCCA                                                                                                             <  2:791183/116‑1
                                                TCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGATTGTTCCGGCTGACTCCGTTGAGCGCCA                                                                                                             >  1:791183/1‑116
                                                 CACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGATTGTTCCGGCTGACT                                                                                                                          <  1:476210/102‑1
                                                 CACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGATTGTTCCGGCTGACT                                                                                                                          >  2:476210/1‑102
                                                        TGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCG                                                                              >  1:250889/1‑139
                                                              ATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAA                                                                                                   >  1:938303/1‑112
                                                              ATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAA                                                                                                   <  2:938303/112‑1
                                                                       GCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCG                                                               <  1:54812/139‑1
                                                                           TGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCA                                                           <  1:373833/139‑1
                                                                               ATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACC                                                       <  2:699369/139‑1
                                                                                 CAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACCGG                                                     >  1:821210/1‑139
                                                                                     AGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGATTGTTCCGGCTGACTCCGTTGAGCGCCAGA                                                                                                           >  1:462895/1‑81
                                                                                     AGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGATTGTTCCGGCTGACTCCGTTGAGCGCCAGA                                                                                                           <  2:462895/81‑1
                                                                                       TCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACCGGAAGGTA                                               >  1:22543/1‑139
                                                                                         TGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACCGGAAGGTAAC                                             >  1:553759/1‑139
                                                                                         TGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACCGGAAGGTAAC                                             >  2:214294/1‑139
                                                                                          GCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACCGGAAGGTAACT                                            <  2:987079/139‑1
                                                                                          GCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACCGGAAGGTAACT                                            <  1:348347/139‑1
                                                                                           CGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACCGGAAGGTAACTT                                           >  2:417578/1‑139
                                                                                           CGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACCGGAAGGTAACTT                                           <  2:503221/139‑1
                                                                                                TCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACCGGAAGGTAACTTACGTA                                      >  2:626425/1‑139
                                                                                                     aaCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACCGGAAGGTAACTTACGTATTGAC                                 >  2:916835/3‑139
                                                                                                          GTAATCTCTGGCGCGTTGCTGATGCGCCGATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACCGGAAGGTAACTTACGTATTGACTATCG                            <  1:361582/139‑1
                                                                                                          GTAATCTCTGGCGCGTTGCTGATGCGCCGATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACCGGAAGGT                                                <  2:1012193/119‑1
                                                                                                          GTAATCTCTGGCGCGTTGCTGATGCGCCGATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACCGGAAGGT                                                >  1:1012193/1‑119
                                                                                                            AATCTCTGGCGCGTTGCTGATGCGCCGATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACCGGAAGGTAACTTACGTATTGACTATCGCA                          <  2:1007040/139‑1
                                                                                                            AATCTCTGGCGCGTTGCTGATGCGCCGATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTG                                                                                  <  2:263398/83‑1
                                                                                                            AATCTCTGGCGCGTTGCTGATGCGCCGATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTG                                                                                  >  1:263398/1‑83
                                                                                                              TCTCTGGCGCGTTGCTGATGCGCCGATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACCGGAAGGTAACTTACGTATTGACTATCGCAGT                        <  1:214294/139‑1
                                                                                                                  TGGCGCGTTGCTGATGCGCCGATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACCGGAAGGTAACTTACGTATTGACTATCGCAGTTGCC                    >  2:1009669/1‑139
                                                                                                                      GCGTTGCTGATGCGCCGATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACCGGAAGGTAACTTACGTATTGACTATCGCAGTTGCCTGGA                <  1:1009669/139‑1
                                                                                                                        GTTGCTGATGCGCCGATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACCGGAAGGTAACTTACGTATTGACTATCGCAGTTGCCTGGAGT              >  2:10138/1‑139
                                                                                                                                  CGCCGATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACCGGAAGGTAACTTACGTATTGACTATCGCAGTTGCCTGGAGTGTGGCACCTG    >  1:10705/1‑139
                                                                                                                                    CCGATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACCGGAAGGTAACTTACGTATTGACTATCGCAGTTGCCTGGAGTGTGGCACCTGCa  >  2:230742/1‑138
                                                                                                                                    CCGATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACCGGAAGGTAACTTACGTATTGACTATCGCAGTTGCCTGGAGTGTGGCACCTGCC  >  2:880347/1‑139
                                                                                                                                                                                                                                                                               
TAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2893826‑2893960
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACCGGAAGGTAACTTACGTATTGACTATCGCAGTTGCCTGGAGTGTGGCACCTGCC  >  NC_000913/2893964‑2894099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.

GATK/CNVnator alignment

N/A