Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	762	35.5	1545288	95.5%	1475750	117.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,984,193	G→A	412 (TAG→TAA)	aslB →	putative AslA‑specific sulfatase‑maturating enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,984,193	0	G	A	100.0%	134.8 / NA	42	412 (TAG→TAA)	aslB	putative AslA‑specific sulfatase‑maturating enzyme
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/20); total (22/20)

GTGCCGGGTATCAGCGTTATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGG  >  NC_000913/3984069‑3984322

gtgCCGGGTATCAGCGTTATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGgccgc                                                                                                                     >  2:377576/1‑139 (MQ=255)

gtgCCGGGTATCAGCGTTATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGgccgc                                                                                                                     >  1:413924/1‑139 (MQ=255)

          tCAGCGTTATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGa                                                                                                           <  1:662312/139‑1 (MQ=255)

                  aTTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGa                                                                                                   >  1:337985/1‑139 (MQ=255)

                     tCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATgc                                                                                                <  2:751695/139‑1 (MQ=255)

                      ccGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATgcg                                                                                               <  2:188554/139‑1 (MQ=255)

                            tCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTaa                                                                                         <  2:648409/139‑1 (MQ=255)

                                ccGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGc                                                                                     <  2:276123/139‑1 (MQ=255)

                                   ccATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCtt                                                                                  >  2:664677/1‑139 (MQ=255)

                                    cATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTa                                                                                 <  1:573246/139‑1 (MQ=255)

                                      tatCTTAATGTATTGGCGGATTTGCTGGCGCACGGTCGCCCGGCCAGCCACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATCCGGCGTAAACGCCTTATc                                                                               <  2:275776/139‑1 (MQ=255)

                                      tatCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATc                                                                               >  1:594360/1‑139 (MQ=255)

                                      tatCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATc                                                                               <  1:664677/139‑1 (MQ=255)

                                        tCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                             <  2:399378/139‑1 (MQ=255)

                                          ttAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                           <  2:378621/139‑1 (MQ=255)

                                             aaGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTa                                                                        >  1:492088/1‑139 (MQ=255)

                                             aaGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTa                                                                        >  2:191575/1‑139 (MQ=255)

                                              aGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                             >  2:428001/1‑133 (MQ=255)

                                              aGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                             <  1:428001/133‑1 (MQ=255)

                                                  aTGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGa                                                                   >  2:679951/1‑139 (MQ=255)

                                                   tGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTa                                                                                 >  2:78992/1‑124 (MQ=255)

                                                   tGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTa                                                                                 <  1:78992/124‑1 (MQ=255)

                                                   tGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGAt                                                                  >  1:298329/1‑139 (MQ=255)

                                                          tttGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                             <  1:319470/121‑1 (MQ=255)

                                                          tttGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                             >  2:319470/1‑121 (MQ=255)

                                                                       ggTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTg                                              <  2:1163/139‑1 (MQ=255)

                                                                            cccGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCg                                         <  1:191575/139‑1 (MQ=255)

                                                                              cGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGtt                                       >  1:272493/1‑139 (MQ=255)

                                                                                   aGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                             <  1:468644/96‑1 (MQ=255)

                                                                                   aGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                             >  2:468644/1‑96 (MQ=255)

                                                                                               catgcGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGgccgc                                                                                                                     <  1:680958/44‑1 (MQ=255)

                                                                                               catgcGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGgccgc                                                                                                                     >  2:680958/1‑44 (MQ=255)

                                                                                                atgcGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                             >  2:238632/1‑83 (MQ=255)

                                                                                                atgcGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                             <  1:238632/83‑1 (MQ=255)

                                                                                                  gcgcATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                             <  1:199185/81‑1 (MQ=255)

                                                                                                  gcgcATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                             >  2:199185/1‑81 (MQ=255)

                                                                                                   cgcATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCGGGATGCGCCGTAAACGCTTTATCGGCCCTACTTGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCg                  >  2:218050/1‑139 (MQ=38)

                                                                                                   cgcATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCg                  >  1:561525/1‑139 (MQ=255)

                                                                                                   cgcATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCg                  >  1:340236/1‑139 (MQ=255)

                                                                                                       tttGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAg              >  2:389125/1‑139 (MQ=255)

                                                                                                        ttGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAgc             >  1:269699/1‑139 (MQ=255)

                                                                                                                   gAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCgg  <  2:561525/139‑1 (MQ=255)

GTGCCGGGTATCAGCGTTATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGG  >  NC_000913/3984069‑3984322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A