Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A15 F58 I0 R3
|
247 |
66.2 |
2815674 |
94.9% |
2672074 |
126.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
CP006881 |
3,772,635 |
A→G |
22.0% |
Y334C (TAT→TGT) |
U712_19455 → |
Protein dltB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP006881 | 3,772,635 | 0 | A | G | 22.0%
| 103.8
/ 31.6
| 59 | Y334C (TAT→TGT) | U712_19455 | Protein dltB |
| Reads supporting (aligned to +/- strand): ref base A (21/25); new base G (6/7); total (27/32) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.30e-01 |
ATGTGTTTATGAGATTCGTATTTTGGATGACTAAGAAAAAGTGGATCAAAAACCGTATGGCCGTCTCAAACATCGGGTATTTCCTGCTGTTTATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATATATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGAATAAGAAATATAAATGGCTGCCGTCCAACCGCTGGACAACCATTCTCGCTATTGTGATCACATTCCATTTCG > CP006881/3772506‑3772769
|
aTGTGTTTATGAGATTCGTATTTTGGATGACTAAGAAAAAGTGGATCAAAAACCGTATGGCCGTCTCAAACATCGGGTATTTCCTGCTGTTTATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATATATCATTTa > 6:127285/1‑139 (MQ=255)
tgtgTTTATGAGATTCGTATTTTGGATGACTAAGAAAAAGTGGATCAAAAACCGTATGGCCGTCTCAAACATCGGGTATTTCCTGCTGTTTATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATATATCATTTa < 3:49123/138‑1 (MQ=255)
tttATGAGATTCGTATTTTGGATGACTAAGAAAAAGTGGATCAAAAACCGTATGGCCGTCTCAAACATCGGGTATTTCCTGCTGTTTATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATGTATCATTTACGGc < 2:223472/138‑1 (MQ=255)
tttATGAGATTCGTATTTTGGATGACTAAGAAAAAGTGGATCAAAAACCGTATGGCCGTCTCAAACATCGGGTATTTCCTGCTGTTTATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATATATCATTTACGGc < 6:122636/138‑1 (MQ=255)
tATGAGATTCGTATTTTGGATGACTAAGAAAAAGTGGATCAAAAACCGTATGGCCTTCTCCACCATCGGGTTTTTCCTGCATTTTATTCTGATGGGGGTTTGTCACGGGCTCGCGCCGCATTATATAATTTaatgc > 5:260910/1‑132 (MQ=255)
tATGAGATTCGTATTTTGGATGACTAAGAAAAAGTGGATCAAAAACCGTATGGCCGTCTCAAACATCGGGTATTTCCTGCTGTTTATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATGTATCATTTACGGCctc > 7:331102/1‑139 (MQ=255)
tGAGATTCGTATTTTGGATGACTAAGAAAAAGTGGATCAAAAACCGTATGGCCGTCTCAAACATCGGGTATTTCCTGCTGTTTATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATATATCATTTACGGCCTCTa < 2:150357/139‑1 (MQ=255)
aTTCGTATTTTGGATGACTAAGAAAAAGTGGATCAAAAACCGTATGGCCGTCTCAAACATCGGGTATTTCCTGCTGTTTATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATATATCATTTACGGCCTCTATCAc > 3:7585/1‑139 (MQ=255)
tCGTATTTTGGATGACTAAGAAAAAGTGGATCAAAAACCGTATGGCCGTCTCAAACATCGGGTATTTCCTGCTGTTTATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATGTATCATTTACGGCCTCTATCACGc > 3:190063/1‑139 (MQ=255)
tCGTATTTTGGATGACTAAGAAAAAGTGGATCAAAAACCGTATGGCCGTCTCAAACATCGGGTATTTCCTGCTGTTTATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATATATCATTTACGGc < 4:216295/128‑1 (MQ=255)
tCGTATTTTGGATGACTAAGAAAAAGTGGATCAAAAACCGTATGGCCGTCTCAAACATCGGGTATTTCCTGCTGTTTATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATATATCATTTACGGc > 3:216295/1‑128 (MQ=255)
ttttGGATGACTAAGAAAAAGTGGATCAAAAACCGTATGGCCGTCTCAAACATCGGGTATTTCCTGCTGTTTATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATGTATCATTTACGGCCTCTATCACGCCGtt < 4:289094/138‑1 (MQ=255)
ttttGGATGACTAAGAAAAAGTGGATCAAAAACCGTATGGCCGTCTCAAACATCGGGTATTTCCTGCTGTTTATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATATATCATTTACGGCCTCTATCACGCCGtt < 7:76457/138‑1 (MQ=255)
gATGACTAAGAAAAAGTGGATCAAAAACCGTATGGCCGTCTCAAACATCGGGTATTTCCTGCTGTTTTTGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATATATCATTTACGGCCTCTATCCCGCCGttgttgt > 2:287395/1‑133 (MQ=255)
aTGACTAAGAAAAAGTGGATCAAAAACCGTATGGCCGTCTCAAACATCGGGTATTTCCTGCTGTTTATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATATATCATTTACGGCCTCTATCACGCCGTTCtgatga < 3:146918/139‑1 (MQ=255)
tGACTAAGAAAAAGTGGATCAAAAACCGTATGGCCGTCTCAAACATCGGGTATTTCCTGCTGTTTATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATATATCATTTACGGCCTCTATCACGCCGTTCTGATGAc < 5:127285/139‑1 (MQ=255)
aaaaCCGTATGGCCGTCTCAAACATCGGGTATTTCCTGCTGTTTATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATATATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGaa < 5:349040/139‑1 (MQ=255)
aaaaCCGTATGGCCGTCTCAAACATCGGGTATTTCCTGCTGTTTATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATATATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGaa < 1:251822/139‑1 (MQ=255)
cagccGTATGGCCGTCTCAAACATCGGGTATTTCCTGCTGTTTATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATATATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAg < 1:155989/136‑1 (MQ=255)
tATGGCCGTCTCAAACATCGGGTATTTCCTGCTGTTTATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATATATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGaa > 3:231624/1‑138 (MQ=255)
tGGCCTTGTCATAAATCGGGTATTTCCTGCTGTTTATGCTGATGGGGGTTTGGCACGGGCTCGCGCCTCAATATATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAAATTTTTCGAGAAGTGGAATa < 6:260910/138‑1 (MQ=255)
tGGCCGTCTCAAACATCGGGTATTTCCTGCTGTTTATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATATATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGAATa < 4:231624/138‑1 (MQ=255)
tgtcAAACATAGGGTATTTACTGCAGTTTATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATATATAATTTACGGCCTCAATCACGCCGGTCATATGACGTGGTAAAACATTTTAGATCAGTGGAATAACaactt > 2:155989/3‑136 (MQ=255)
aaaCATCGGGTATTTCCTGCTGTTTATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATGTATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGAATAAGAAATATaaa < 8:331102/139‑1 (MQ=255)
aTCGGGTATTTCCTGCTGTTTATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATGTATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGAATAAGAAATATAAATGGc < 4:190063/139‑1 (MQ=255)
cGGGTATTTCCTGCTGTTTATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATGTATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGAATAAGAAATATAAATGGCTg > 4:128795/1‑139 (MQ=255)
cGGGTATTTCCTGCTGTTTATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATATATCATTTACGGCCTCTATCACGCCGTGCTGATGACGTGTTACATCTTTTTCGAGAAGTGGAATAAGAAATATAAATGGCTg > 5:29218/1‑139 (MQ=255)
ggTATTTCCTGCTGTTTATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATATATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGAATAAGaa > 2:63193/1‑124 (MQ=255)
ggTATTTCCTGCTGTTTATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATATATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGAATAAGaa < 1:63193/124‑1 (MQ=255)
ggTATTTCCTGCTGTTTATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATATATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGAATAAGAAATATAAATGGc < 7:112062/135‑1 (MQ=255)
ggTATTTCCTGCTGTTTATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATATATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGAATAAGAAATATAAATGGc > 8:112062/1‑135 (MQ=255)
ttCCTGCTGTTTATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATATATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGAATAAGAAATATAAATGGCTGCCGTcc < 4:7585/138‑1 (MQ=255)
ctgTTTATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATGTATCATTTACGGCCTCTATCACGCCGTTCtgatga < 8:31351/79‑1 (MQ=255)
ctgTTTATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATGTATCATTTACGGCCTCTATCACGCCGTTCtgatga > 7:31351/1‑79 (MQ=255)
ctgTTTATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATATATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGAATAAGAAATATAAATGGCTGCCGTCCAACCg < 7:26105/137‑1 (MQ=255)
ctgTTTATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATATATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGAATAAGAAATATAAATGGCTGCCGTCCAACCg > 8:26105/1‑137 (MQ=255)
tATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATGTATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGAATAAGAAATATAAATGGCTGCCGTCCAACCGCTGGAc > 8:210901/1‑138 (MQ=255)
tATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATGTATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGAATAAGAAATATAAATGGCTGCCGTCCAACCGCTGGAc < 7:210901/138‑1 (MQ=255)
tATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATGTATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGAATAAGAAATATAAATGGCTGCCGTCCAACCGCTGGAc > 5:257833/1‑138 (MQ=255)
tATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATATATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGAATAAGAAATATAAATGGCTGCCGTCCAACCGCTGGACa > 6:91330/1‑139 (MQ=255)
tGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATATATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGAATAAGAAATATAAATGGCTGCCGTCCAACCGCTGGACaa < 2:183026/138‑1 (MQ=255)
tGCTGATGGGGGGTTGGCACGGGCTCGCGCCGCAATATATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGAATAAGAAATATAAATGGCTGCCGTCCAACCGCTGGACaa < 8:304793/138‑1 (MQ=255)
ggCTGATGGGGGGTTGGCACGGGCTCGCGCCGCAATATATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGAATAAGAAATATAAATGGCTGCCGTCCAACCGCTGGACaa < 4:234247/137‑1 (MQ=255)
gATGGGGGTTTGGCACGGGCTCGCGCCGCAATATATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGAATAAGAAATATAAATGGCTGCCGTCCAACCg > 1:124/1‑126 (MQ=255)
gATGGGGGTTTGGCACGGGCTCGCGCCGCAATATATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGAATAAGAAATATAAATGGCTGCCGTCCAACCg < 2:124/126‑1 (MQ=255)
gATGGGGGTTTGGCACGGGCTCGCGCCGCAATATATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGAATAAGAAATATAAATGGCTGCCGTCCAACCGCTGGACAACCAtt > 5:273408/1‑139 (MQ=255)
aTGGGGGTTTGGCACGGGCTCGCGCCGCAATGTATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGAATAAGAAATATAAATGGCTGCCGTCCAACCGCTGGACAACCAtt < 6:257833/138‑1 (MQ=255)
tGGGGGTTTGGCACGGGCTCGCGCCGCAATATATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGAATAAGAAATATAAATGGCTGCCGTCCAACCGCTGGACAACCATtct > 1:964/1‑139 (MQ=255)
ggggTTTGGCACGGGCTCGCGCCGCAATATATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGAATAAGAAATATAAATGGCTGCCGTCCAACCGCTGGACAACCATTCTCg < 6:273408/139‑1 (MQ=255)
ggCACGGGCTCGCGCCGCAATATATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGAATAAGAAATATAAATGGCTGCCGTCCAACCGCTGGACAACCAt > 1:282165/1‑127 (MQ=255)
ggCACGGGCTCGCGCCGCAATATATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGAATAAGAAATATAAATGGCTGCCGTCCAACCGCTGGACAACCAt < 2:282165/127‑1 (MQ=255)
ggCACGGGCTCGCGCCGCAATATATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGAATAAGAAATATAAATGGCTGCCGTCCAACCGCTGGACAACCATTCTCGCTATtgt > 6:258829/1‑139 (MQ=255)
gggCTCGCGCCGCAATATATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGAATAAGAAATATAAATGGCTGCCGTCCAACCGCTGGACAACCATTCTCGCTATTGTGATca < 5:258829/139‑1 (MQ=255)
gCTCGCGCCGCAATATATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGAATAAGAAATATAAATGGCTGCCGTCCAACCGCTGGACAACCATTCTCGCTATTGTGATcaca < 7:102792/139‑1 (MQ=255)
gcgcCGCAATATATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGAATAAGAAATATAAATGGCTGCCGTCCACCCGCTGGACAACCATTCTCGCTATTGTGATCACATTc > 2:70722/1‑138 (MQ=255)
gcgcCGCAATATATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGAATAAGAAATATAAATGGCTGCCGTCCAACCGCTGGACAACCATTCTCGCTATTGTGATCACATTc > 5:27735/1‑138 (MQ=255)
cAATATATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGAATAAGAAATATAAATGGCTGCCGTCCACCCGCTGGACAACCATTCTCGCTATTGTGATCCCATTCCATTTCg > 6:184474/1‑139 (MQ=255)
cAATATATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGAATAAGAAATATAAATGGCTGCCGTCCAACCg < 1:86105/98‑1 (MQ=255)
cAATATATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGAATAAGAAATATAAATGGCTGCCGTCCAACCg > 2:86105/1‑98 (MQ=255)
|
ATGTGTTTATGAGATTCGTATTTTGGATGACTAAGAAAAAGTGGATCAAAAACCGTATGGCCGTCTCAAACATCGGGTATTTCCTGCTGTTTATGCTGATGGGGGTTTGGCACGGGCTCGCGCCGCAATATATCATTTACGGCCTCTATCACGCCGTTCTGATGACGTGTTACAACTTTTTCGAGAAGTGGAATAAGAAATATAAATGGCTGCCGTCCAACCGCTGGACAACCATTCTCGCTATTGTGATCACATTCCATTTCG > CP006881/3772506‑3772769
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
