Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 113 306.8 9564096 98.5% 9420634 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,723,067 1 bp→AT intergenic (‑181/‑120) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,723,0661.A84.1% 673.8 / 135.7 226intergenic (‑180/‑121)U712_19190/U712_19195PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base . (19/17);  new base A (94/96);  total (113/113)
Fisher's exact test for biased strand distribution p-value = 8.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.39e-01
*CP0068813,723,0670GT84.1% 585.5 / 93.9 226intergenic (‑181/‑120)U712_19190/U712_19195PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (94/96);  minor base A (19/17);  total (113/113)
Fisher's exact test for biased strand distribution p-value = 8.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.59e-01

Only 100 of 237 total aligned reads displayed.

CTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAACT‑GTTTTTTAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGAAACTCAAGCATTGCAAAAAGTTGAA  >  CP006881/3722921‑3723216
                                                                                                                                                   ||                                                                                                                                                    
cTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACT‑Attt                                                                                                                                                    >  2:1839280/1‑150 (MQ=255)
 tAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACT‑Atttt                                                                                                                                                   >  2:894723/1‑150 (MQ=255)
 tAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACT‑Atttt                                                                                                                                                   <  2:4369318/150‑1 (MQ=255)
  aaaaTTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAAAACGCTTTCATCATAAAACATGATAGCGTTTTCGTCAACT‑Attttt                                                                                                                                                  >  1:4359606/1‑150 (MQ=255)
  aaaaTTCGACAACGAATAAAGGACACAGCGTTCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACT‑Attttt                                                                                                                                                  <  1:1778064/150‑1 (MQ=255)
   aaaTTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACT‑Atttttt                                                                                                                                                 <  1:617019/150‑1 (MQ=255)
   aaaTTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACT‑Atttttt                                                                                                                                                 <  1:936038/150‑1 (MQ=255)
   aaaTTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACT‑Atttttt                                                                                                                                                 <  2:2308552/150‑1 (MQ=255)
    aaTTCGCCAACGAATAAAGGTCACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGAATAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACT‑Attttctt                                                                                                                                                >  1:4507813/1‑147 (MQ=255)
    aaTTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACT‑Attttttt                                                                                                                                                <  2:2051834/150‑2 (MQ=255)
     aTTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACT‑Attttttta                                                                                                                                               <  2:312667/150‑3 (MQ=255)
      ttCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACT‑Atttttttag                                                                                                                                              >  1:4104140/1‑147 (MQ=255)
      ttCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACT‑Atttttttag                                                                                                                                              >  1:2611518/1‑147 (MQ=255)
       tCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACT‑Atttttttagt                                                                                                                                             <  2:1827839/150‑5 (MQ=255)
         gCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTa                                                                                                                                            >  1:3612681/1‑150 (MQ=255)
          ccAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTaa                                                                                                                                           >  2:3685308/1‑150 (MQ=255)
           cAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTaaa                                                                                                                                          >  1:4567573/1‑150 (MQ=255)
            aaCGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAAt                                                                                                                                         <  2:1690366/150‑1 (MQ=255)
            aaCGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAAt                                                                                                                                         <  2:110799/150‑1 (MQ=255)
            aaCGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAACCCCGCTTATCCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAAt                                                                                                                                         <  1:4186858/150‑1 (MQ=255)
             aCGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATa                                                                                                                                        <  1:3969146/150‑1 (MQ=255)
              cGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAg                                                                                                                                       >  1:2585425/1‑150 (MQ=255)
              cGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAg                                                                                                                                       <  2:17561/150‑1 (MQ=255)
                aaTAAAGGTCACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGtt                                                                                                                                     <  1:3658473/150‑1 (MQ=255)
                aaTAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGtt                                                                                                                                     >  1:3466414/1‑150 (MQ=255)
                   aaaGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGa                                                                                                                                  >  1:2275955/1‑150 (MQ=255)
                   aaaGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGa                                                                                                                                  >  1:1018435/1‑150 (MQ=255)
                      ggACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATat                                                                                                                               <  2:3624123/150‑2 (MQ=255)
                        acacAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGGTataa                                                                                                                             >  2:947649/1‑150 (MQ=255)
                         cacaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGAtgtaac                                                                                                                            >  1:1897107/1‑145 (MQ=255)
                           caGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAAcat                                                                                                                          >  2:1939870/1‑148 (MQ=255)
                           caGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAAcat                                                                                                                          <  2:1654287/150‑3 (MQ=255)
                           caGCGATCCTTTTTCATTTACGCAAATTTTAGGCCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTACATCATAAATCATGAAAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAAcat                                                                                                                          <  1:2705200/150‑3 (MQ=255)
                             gCGATCCTTTTTCATTTACGCATATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTCCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAAcatgt                                                                                                                        <  1:4715137/150‑5 (MQ=255)
                             gCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAAcatgt                                                                                                                        >  2:1422903/1‑146 (MQ=255)
                             ccGATCCTTTTTCATTTCCGCAACTTTTAGGCCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGATTACGCTTCCATCATCAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAAcatgt                                                                                                                        <  1:2165374/149‑5 (MQ=255)
                                    tttttCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAAAACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACaaa                                                                                                                 >  2:1580802/1‑150 (MQ=255)
                                        tCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAAtt                                                                                                             <  2:807090/150‑1 (MQ=255)
                                        tCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAAtt                                                                                                             >  1:145805/1‑150 (MQ=255)
                                           tttAGGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCGCAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGt                                                                                                          <  2:1213902/150‑1 (MQ=255)
                                           tttACGCAAATCTTAGGTCTTGCCTGCTTTACCAGGCACAATCCCGCTTATTCAGATTAAGAATCCGCTTTCATCATGAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGt                                                                                                          <  1:4410682/150‑1 (MQ=255)
                                            gtACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTa                                                                                                         <  1:1714284/149‑1 (MQ=255)
                                             tACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTaa                                                                                                        <  1:4168152/150‑1 (MQ=255)
                                              aCGCAACTTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTaaa                                                                                                       <  1:3997859/150‑1 (MQ=255)
                                                 cAAACTTTAGGTCTTGCCTGCTTTACCAGCCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTaaaaat                                                                                                    <  2:3744746/150‑2 (MQ=255)
                                                  aaaTTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTaaaaatt                                                                                                   >  2:3787449/1‑148 (MQ=255)
                                                     ttttAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaat                                                                                                <  2:1533664/150‑3 (MQ=255)
                                                      tttAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTCCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAaattaatt                                                                                               <  1:1258087/150‑1 (MQ=255)
                                                          ggTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATCAATTGTGa                                                                                           >  2:1351571/1‑150 (MQ=255)
                                                            tCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaa                                                                                         <  2:716183/150‑1 (MQ=255)
                                                             cTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaat                                                                                        <  1:3581907/150‑2 (MQ=255)
                                                               tGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTCCATCCTAGATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATAc                                                                                      <  2:3889580/150‑1 (MQ=255)
                                                                   tGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                                                  >  2:3080878/1‑150 (MQ=255)
                                                                   tGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGAGAAATACTTca                                                                                  >  2:3302071/1‑150 (MQ=255)
                                                                    gCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTcac                                                                                 >  2:4287975/1‑150 (MQ=255)
                                                                     ccttACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGCCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTcaca                                                                                <  1:1106963/148‑1 (MQ=255)
                                                                       ttACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAat                                                                              >  1:3509902/1‑150 (MQ=255)
                                                                          ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                                           <  2:4404630/150‑1 (MQ=255)
                                                                          ccAGTCACAATCCCGCTTATCCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                                           <  2:3412223/150‑1 (MQ=255)
                                                                           cAGTCACAATCCCGCTTATTCAGAGTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTCCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCg                                                                          <  2:2979081/150‑1 (MQ=255)
                                                                            aGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGt                                                                         <  1:870605/150‑1 (MQ=255)
                                                                            aGTCACAATCCCGCTTATTCAGATCAAGAATACGCTTTCATCATAAATCATGATAGCCTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAACATCGt                                                                         >  1:973969/1‑150 (MQ=255)
                                                                              tCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGc                                                                       >  1:4053126/1‑150 (MQ=255)
                                                                               cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCt                                                                      >  1:3776724/1‑150 (MQ=255)
                                                                                    tCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACt                                                                 <  1:3867694/150‑1 (MQ=255)
                                                                                       cGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATg                                                              >  2:1966343/1‑150 (MQ=255)
                                                                                        gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGc                                                             >  2:1274569/1‑150 (MQ=255)
                                                                                                  aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAtga                                                   <  2:1897107/150‑3 (MQ=255)
                                                                                                   ttaagaATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaa                                                  >  1:2913323/4‑150 (MQ=255)
                                                                                                      agaATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaaga                                               >  2:213480/1‑150 (MQ=255)
                                                                                                        aaTACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATgaaagaaa                                             >  2:3994012/1‑150 (MQ=255)
                                                                                                         aTACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaagaaag                                            >  2:1035598/1‑150 (MQ=255)
                                                                                                          tACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGc                                           <  1:3033213/150‑1 (MQ=255)
                                                                                                           aCGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGGTCAATCATGaaagaaagga                                          >  2:4194424/1‑148 (MQ=255)
                                                                                                               tttCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGaa                                      >  2:1186488/1‑150 (MQ=255)
                                                                                                                ttCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAACTTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGaaa                                     <  2:1677160/150‑1 (MQ=255)
                                                                                                                ttCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGaaa                                     >  2:4469085/1‑150 (MQ=255)
                                                                                                                 tcatcaTAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGaaaa                                    <  1:3685308/150‑1 (MQ=255)
                                                                                                                     catAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACa                                <  1:2787190/150‑1 (MQ=255)
                                                                                                                        aaaTCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATg                             <  1:1767817/150‑1 (MQ=255)
                                                                                                                         aaTCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCGATCATGAAAGAAAGCAGGAAAAGACAATgg                            <  2:4337288/150‑1 (MQ=255)
                                                                                                                          atcaTGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGGAAGAAAGCAGGAAAAGACAATGGa                           >  2:2128573/4‑150 (MQ=255)
                                                                                                                           tcaTGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGaa                          <  1:1133084/148‑1 (MQ=255)
                                                                                                                           tcaTGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGaa                          >  2:3111411/3‑150 (MQ=255)
                                                                                                                              tGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACt                       >  1:2399731/1‑150 (MQ=255)
                                                                                                                               gATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTc                      <  2:2275955/150‑1 (MQ=255)
                                                                                                                                aTAGCGTTCCCGTCAACTATTTTTTTAGTAAAATAGTTTGATATATCTTGTAGACAGACATTCGTAAAAAGTCATTGTGAAATACTTCACAATAGCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCa                     <  1:1428860/150‑1 (MQ=255)
                                                                                                                                  aGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGGCAATGGAAACTCAAg                   >  1:4437369/1‑150 (MQ=255)
                                                                                                                                  aGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAg                   >  1:668976/1‑150 (MQ=255)
                                                                                                                                    cgatttCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCa                 <  2:4507813/147‑1 (MQ=255)
                                                                                                                                    cGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCa                 <  2:2611518/150‑1 (MQ=255)
                                                                                                                                    cGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCa                 >  2:3319151/1‑150 (MQ=255)
                                                                                                                                     gTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGACAGGACAAGGGAGACTCCAGCAt                >  2:1147249/1‑150 (MQ=255)
                                                                                                                                      ttttCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACATAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCAtt               <  2:927980/150‑1 (MQ=255)
                                                                                                                                       tttCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAGGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCAtta              >  1:2117635/1‑149 (MQ=255)
                                                                                                                                          cGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACaa           <  1:3385650/150‑1 (MQ=255)
                                                                                                                                               actatttttttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGt      >  1:2022190/6‑150 (MQ=255)
                                                                                                                                                ctatttttttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGtt     >  2:2956813/5‑150 (MQ=255)
                                                                                                                                                 tatttttttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTCTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGATAAGACAATGGAAACTCAAGCATTACAAAAGGTTg    <  1:4084155/147‑1 (MQ=255)
                                                                                                                                                   tttttttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGaa  <  1:2884377/149‑1 (MQ=255)
                                                                                                                                                   ||                                                                                                                                                    
CTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAACT‑GTTTTTTAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGAAACTCAAGCATTGCAAAAAGTTGAA  >  CP006881/3722921‑3723216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A